Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Polyphenol and Anti-oxidant Effects of Kalopanax septemlobus Koidz. Leaf Extracts

음나무(Kalopanax septemlobus Koidz.) 잎 추출물의 폴리페놀 및 항산화효과

  • Jun, Dong-Ha (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Jin-Tae (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Cheon, Soon-Ju (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Chang-Eon (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Kim, Tae-Hoon (Department of Herbal Medicinal Pharmacology, Daegu Haany University) ;
  • Lee, Do-Hyung (Department of Forest Resources, Yeungnam University) ;
  • Han, Jin-Gyu (Korea Forest Research Institute) ;
  • Kim, Sea-Hyun (Korea Forest Research Institute)
  • 전동하 (대구한의대학교 화장품약리학과) ;
  • 이진태 (대구한의대학교 화장품약리학과) ;
  • 천순주 (대구한의대학교 화장품약리학과) ;
  • 이창언 (대구한의대학교 화장품약리학과) ;
  • 김태훈 (대구한의대학교 한약재약리학과) ;
  • 이도형 (영남대학교 산림자원학과) ;
  • 한진규 (국립산림과학원) ;
  • 김세현 (국립산림과학원)
  • Published : 2009.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of the study was to assess the cosmeceutical activity of Kalopanax septemlobus leaf and it is possible that can be used as a cosmetic ingredient for applicaiton of cosmetic industries. The concentration of total phenolic compound of hot water and 70% EtOH extracts of K. septemlobus leaf showed 104 mg/L and 125 mg/L respectively. In the result of DPPH(1,1- diphenyl-2-picryl -hydrazyl) scavenging radical activity, 70% EtOH extracts of K. septemlobus leaf showed 93.1% and it was similar to BHA(butylated hydroxyanisole) effect at 1,000ppm concentration. Xanthine oxidase inhibition of hot water extracts and 70% EtOH extracts of K. septemlobus leaf were 46.6% and 60.4% at 1,000ppm, respectively. In these results, K. septemlobus leaf has a great potential as a cosmeceutical ingredient with a natural anti-oxidant source.

음나무 잎 추출물의 생리활성을 분석하기 위해 열수추출물과 70% EtOH 추출물의 폴리페놀의 함량을 측정하였으며, 항산화활성 분석을 위해 전자공여능, nitric oxide radical 저해활성, xanthine oxidase 저해활성, ABTS radical cation decolorization을 측정하였다. 폴리페놀의 총 함량은 열수추출물과 70% EtOH 추출물에서 각각 104 mg/L, 125 mg/L으로 정량되어 70% EtOH 추출물이 열수추출 물에 비해 높은 함량을 보였다. DPPH radical 소거능은 70% EtOH 추출물이 1,000 ppm에서 93.1%로 나타나 같은 농도의 BHA와 유사한 소거능을 보였다. nitric oxide radical 저해활성은 70% EtOH 추출물과 열수추출물이 1,000 ppm에서 49.6%, 49.5%로 나타나 같은 농도의 BHA보다 높은 nitrit oxide radical 저해활성을 보였다. Xanthine oxidase 저해활성은 1,000 ppm농도에서 열수 추출물과 70% EtOH 추출물에서 각각 46.6%, 60.4% 활성을 나타내었다. ABTS radical cation decolorization 활성은 1,000 ppm에서 열수추출물이 92.7%, 70% EtOH 추출물이 91.4%로 나타났다. 이상의 결과로 보아 음나무 잎의 70% EtOH 추출물과 열수추출물 모두 많은 폴리페놀이 함유되어 있으며, 70% EtOH 추출물과 열수추출물 모두 합성항산화제에 비해 항산화 활성이 다소 낮지만 천연 항 산화제로 우수한 항산화활성을 나타내는 것으로 분석되었다. 이를 바탕으로 음나무 잎의 강한 항산화능을 피부노화 예방관련 미용식품 및 기능성 화장품 소재로 활용 가능한 유용한 수종으로 개발 될 수 있을 것이다.

Keywords

References

  1. 김세현, 박영기, 장용석, 한진규, 정현관. 2007. 음나무 추출물 의 세포 내 산화 스트레스와 항산화 활성. 한국목재공학회. 35(6): 126-134
  2. 문흥규, 박소영, 김용욱, 손석규, 김지아. 2007. 산림자원의 기내대량증식. 국립산림과학원. pp. 39
  3. 박희준, 남정환, 정현주, 김원배, 박광균, 정원윤, 최종원. 2005. 음나무 잎 수피의 진통소염효과 및 아주반트로 유발된 산화적 스트레스에 대한 효과. 생약학회지. 36(4): 18-323
  4. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature. 181: 1199-1200 https://doi.org/10.1038/1811199a0
  5. Brand-Wiliams, W., Cuvelier M. E. and C. Berset. 1995. Use of a free radical method to evaluate antioxidant activity. Technology. 28: 25-30
  6. Choi, S. W. 1997. Antioxidative Properties of Methanolic Extracts in Leaves of Kalopanax pictum Nakai. Research Bulletin of the Catholic University of Taegu-Hyosung. 54(2): 131-139
  7. Hatano, T., Yasuhara, T., Fukuda, T., Noro, T. and T. Okuda. 1989. Phenolic constituents of licoriceⅡ. Structures of licopyranocoumarin, licoarylcoumarin and glisoflavone, and inhibitory effects of licorice phenolics on xanthine oxidase. Chemical & Phamaceutical Bulletin. 37: 3005-3009 https://doi.org/10.1248/cpb.37.3005
  8. Hwang, H. S., Lee, Y. K. and K. G. Lee. 2006. Fractionation of Banaba Leaves Extract(Lagerstroemia speciosa L. Pers) and its Antioxidant Activity. Food Engineering Progress. 10: 120-124
  9. Jung, M. S., Lee G. S. and Chae H. J. 2004. In vitro biological activity assay of ethanol extract of Radish. Journal of Korean Society for Applied Biological Chemistry. 47(1): 67-71
  10. Kelley, W. N. and J. B. Wyngaarden. 1974. Enzymology of gout. Advances in Enzymology and Related Areas of Molecular Biology. 41: 1-33
  11. Kong, W. S. 2004. Species Composition and Distribution of Native Korean Conifers. Korean J. Geographical Society. 39: 528-543
  12. Lee, S. Y., Hwang E. J., Kim G. H., Choi Y. B., Lim C. Y., S. M. Kim. 2005. Antifungal and antioxidant activities of extracts from leaves and flowers of Camellia japonica L. Korea Journal of Food Science and Technology. 13(3): 93-100
  13. Marcocci, L., Maguire J. J., Droylefaix M. T. and L. Packer. 1994. The Nitric Oxide Scavenging Properties of Ginkgo biloba Extract EGb 761. Biochemical and Biophysical Research Communications. 201, 15: 748-755 https://doi.org/10.1006/bbrc.1994.1764
  14. Maxwell, S. J. 1995. Prospects for the use of anti-oxidant therapies. Drugs. 49: 345-361 https://doi.org/10.2165/00003495-199549030-00003
  15. Pellegrin, N., Re, R., Yang, M and Rice-Evans, C. 1998. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activites applying 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Method Enzymol. 299: 379-389
  16. Stirpe, F. and E. D. Corte. 1969. The regulation of rat liver xanthine oxidase. Journal of Biochemistry. 244:3855-3861
  17. Storch, J. and E. Ferber. 1988. Detergent-amplified chemiluminescence of lucigenin for determination of superoxide anion production by NADPH oxidase and xanthine oxidase. Analytical Biochemistry. 169(2):262-267 https://doi.org/10.1016/0003-2697(88)90283-7
  18. Swain, T., Hillis, W.E. and Ortega, M. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. Science of Food Agriculture. 10:83-88
  19. Wyngaarden, J. B. and E. W. Jr. Holmes. 1977. Molecular nature of enzyme regulation in purine biosynthesis. Ciba Foundation Symposium. 48: 43-64