Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Estimation of Genetic Characteristic and Cumulative Power of Discrimination using the Microsatellite Markers in Korean Native Chicken

Microsatellite Marker를 사용한 재래 닭 품종 유전적 특성 및 개체 식별력 분석

  • Lee, Kun-Woo (Genomic Informatics Center, Hankyong National University) ;
  • Oh, Jae-Don (Genomic Informatics Center, Hankyong National University) ;
  • Lee, Jin-Ah (Genomic Informatics Center, Hankyong National University) ;
  • Cho, Kyu-Ho (National Institute of Animal Science) ;
  • Nam, In-Sik (Korea Livestock Products HACCP Accreditation Service) ;
  • Lee, Jun-Heon (Chungnam National University) ;
  • Seo, Ok-Suk (National Institute of Animal Science) ;
  • Jeon, Gwang-Joo (Genomic Informatics Center, Hankyong National University) ;
  • Lee, Hak-Kyo (Genomic Informatics Center, Hankyong National University) ;
  • Kong, Hong-Sik (Genomic Informatics Center, Hankyong National University)
  • Received : 2010.03.11
  • Accepted : 2010.03.18
  • Published : 2010.03.31
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Abstract

To estimate the genetic characteristics and cumulative power of discrimination (CPD) Korean Native Chicken. We used a total of 195 genomic DNAs from four breeds population (Korean Native Red chicken: R, Korean Native Yellow chicken: Y, Korean Native Black chicken: L, Ogal chicken: S). Frequencies of microsatellites markers were used to estimate heterozygosities and genetic distances. The lowest distance (0.05) was observed between the R and L strains and the highest distance (0.158) between the L and S strains. Korean native chicken strains (R, Y, K) have each other comparatively near genetic distance. Cumulative power of discriminate (CPD) was 99.999% by including the 10 microsatellites loci individual identification system. And then matching probability in that two different individuals incidentally have same genotype was estimated to $0.36{\times}10^{-7}$. The system employing the 10 markers therefore provided to be applicable to individual identification in Korea native chicken.

본 연구는 10종의 MS(microsatellite) Marker를 이용하여 한국 재래닭 품종 내 외모 특성에 의해 구분되는 3계통(적갈색: 60수, 황갈색: 46수, 흑색: 40수)과 오골계 집단(49수), 총 4계통(총 195수)에 대한 유전 특성을 분석하고, 이를 활용하여 동일성 검정을 실시할 경우 개체 식별에 대한 신뢰도를 검정하기 위해 실시하였다. 집단 간의 유전적 유연관계를 알아보기 위해 각 MS marker별 대립 유전자의 빈도를 산출하여 이를 근거로 집단간의 보정을 통한 방법을 이용하는 DISPAN program을 활용하여 유전적 거리에 대한 추정 결과 R(적갈색 계통)과 L(흑색 계통)간의 유전적 거리는 0.05로 가장 가까운 것으로 나타났으며, R과 Y(황갈색 계통, 0.073), Y과 L(0.083) 역시 가까운 유전적 거리를 나타내고 있음을 확인하였다. 반면, 재래닭 집단과 오골계 집단(S) 간의 유전적 거리는 0.149(R과 S), 0.144(Y과 S) 그리고 0.158(L과 S)으로 비교적 먼 것으로 나타나 재래닭 집단과 오골계 집단 간의 유전적 차별성을 확인할 수 있었다. 10종의 MS marker를 대상으로 누적 개체 식별력을 계산한 결과 99.999%로 확인되었고 $0.255{\times}10^{-7}$의 짝확률 값이 추정되었다.

Keywords

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