Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Antioxidant and Anticancer Activities of Lotus (Nelumbo nucifera) Leaf and Root

연(蓮) 잎과 뿌리의 항산화 및 항암활성

  • Jeong, Chang-Ho (Division of Applied Life Sciences, Institute of Agriculture Life Sciences, Graduate School, Gyeongsang National University) ;
  • Son, Ki-Bong (Division of Applied Life Sciences, Institute of Agriculture Life Sciences, Graduate School, Gyeongsang National University) ;
  • Kim, Jin-Hee (Division of Applied Life Sciences, Institute of Agriculture Life Sciences, Graduate School, Gyeongsang National University) ;
  • Kang, Sun-Kyung (Division of Applied Life Sciences, Institute of Agriculture Life Sciences, Graduate School, Gyeongsang National University) ;
  • Park, Eun-Young (Division of Applied Life Sciences, Institute of Agriculture Life Sciences, Graduate School, Gyeongsang National University) ;
  • Seo, Kwon-Il (Department of Food and Nutrition, Sunchon National University) ;
  • Shim, Ki-Hwan (Division of Applied Life Sciences, Institute of Agriculture Life Sciences, Graduate School, Gyeongsang National University)
  • 정창호 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 손기봉 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 김진희 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 강선경 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 박은영 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 서권일 (순천대학교 식품영양학과) ;
  • 심기환 (경상대학교 응용생명과학부.농업생명과학연구원)
  • Published : 2010.02.27
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Abstract

To obtain basic data on the use of lotus as a raw material in functional food, antioxidant and anticancer activities of the leaf and root were investigated. Total flavonoid and total phenolic contents, at 12.84 mg/g and 24.33 mg/g respectively, were higher in white lotus leaf (WLL) than in any other part of the plant. The radical-scavenging activity of different tissues of lotus, measured in the DPPH radical-scavenging assay, increased with higher concentrations of solvent fractions. The butanol fraction of white lotus leaf showed the highest DPPH radical-scavenging activity. The reducing power of fractions increased in a dose-dependent manner. The butanol fraction of WLL had the greatest reducing power, and showed strong antioxidant activity in the linoleic acid system, and high-level inhibition of tyrosinase. Fractions from lotus were also capable of scavenging nitrite, depending on the concentration of the fractions. Butanol fractions of the leaf of white and red lotus scavenged 95.61% and 92.15% of available nitrite, respectively, when used at 1 mg/mL concentrations. Butanol fractions from leaf of white and red lotus exhibited the strongest inhibitory effects on human lung and colon cancer cells.

연을 새로운 기능성 식품의 재료로 활용하기 위하여 잎과 뿌리로 구분한 후 항산화 및 항암활성을 측정한 결과는 다음과 같다. 총 플라보노이드와 총 페놀릭 화합물은 백련 잎에서 각각 12.84 mg/g 및 24.33 mg/g으로 높게 나타났다. 연의 부위별 DPPH radical 소거활성을 측정한 결과 분획물의 농도가 증가함에 따라 DPPH radical 소거활성이 증가하는 경향을 보였으며, 특히 백련잎의 부탄올 분획물에서 가장 높은 DPPH radical 소거활성을 보였다. 환원력도 분획물의 농도가 증가함에 따라 환원력이 증가하는 경향을 보였으며, 환원력 또한 백련잎의 부탄올 분획물에서 가장 높게 나타났다. Linoleic acid를 이용한 자동산화 억제활성을 실험한 결과 다른 분획물에 비하여 잎의 부탄올 분획물에서 가장 높은 과산화 억제활성을 나타내었고, tyrosinase 저해 활성 역시 잎의 부탄올 분획물에서 가장 높은 활성을 보였다. 연의 부위별 아질산염 소거활성을 측정한 결과 분획물의 농도가 증가함에 따라 아질산염 소거활성 역시 증가하는 경향을 보였으며, 특히 백련과 홍련잎 부탄올 분획물을 1mg/mL의 농도로 첨가하였을 때 각각 95.61%와 92.15%의 높은 아질산염 소거활성을 보였다. 폐암세포와 결장암세포에 대한 생육억제활성을 측정한 결과 백련과 홍련잎의 부탄올 분획물에서 가장 높은 생육억제활성을 보였다.

Keywords

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