Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antimicrobial Activity of Fruit of Crataegus pinnatifida Bunge against Multidrug Resistant Pathogenic Pseudomonas aeruginosa and Candida sp.

항생제 다제내성 Pseudomonas aeruginosa 및 Candida 균주에 대한 산사자의 항균 활성

  • Ryu, Hee-Young (Dept. of Food and Nutrition, Andong National University) ;
  • Ahn, Seon-Mi (Dept. of Food and Nutrition, Andong National University) ;
  • Kim, Jong-Sik (Dept. of Biological Science, School of Bioresource Science, Andong National University) ;
  • Jung, In-Chang (Dept. of Food and Nutrition, Andong National University) ;
  • Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
  • 류희영 (안동대학교 식품영양학과) ;
  • 안선미 (안동대학교 식품영양학과) ;
  • 김종식 (안동대학교 생명과학과) ;
  • 정인창 (안동대학교 식품영양학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Received : 2009.12.17
  • Accepted : 2010.03.08
  • Published : 2010.03.28
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Abstract

The fructus of Crataegus pinnatifida Bunge (CBF) has been used as medicinal and food source in worldwide. In this study, antimicrobial activity of the methanol extract and its sequential organic solvent fractions of CBF against different pathogenic bacteria and fungi, including multidrug resistant Pseudomonas aeruginosa and Candida sp., were investigated. The methanol extract of CBF was active against various gram-positive and gram-negative bacteria, and the ethylacetate and butanol fractions of CBF showed strong antibacterial activity against Listeria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Salmonella typhimurium, Proteus vulgaris, Escherichia coli and various multidrug resistant Pseudomonas aeruginosa with minimal inhibitory concentration of 1.0~7.5 mg/mL. Also the fractions showed anti-Candida activity against C. albicans, C. kruseis and C. geochares. The methanol extract of CBF and its solvent fractions, except n-hexane fraction, did not show any hemolytic activity against human red blood cell up to $500\;{\mu}g/mL$, respectively. The hemolysis in n-hexane fraction at $500\;{\mu}g/mL$ was less than 9.9%. Our results suggest that the CBF could be developed as a potent antibacterial agent, especially for multidrug resistant Pseudomonas aeruginosa.

산사자는 전 세계적으로 이용되는 있는 식용/약용 생물자원 중 하나이다. 본 연구에서는 산사자의 유용 생리활성 검토를 위한 연구의 일환으로, 산사자의 methanol 추출물 및 이의 n-hexane, ethylacetate, butanol 분획물 및 물 잔류물을 조제하여 항생제 다제내성 Pseudomonas aeruginosa 및 Candida sp.를 포함하는 다양한 병원성 및 식중독 미생물에 대한 항균활성을 평가하였다. 산사자의 methan이 추출물은 그람 양성 및 음성의 다양한 세균에 대해 항균활성을 나타내였고, 이의 분획물 중 ethylacetate 및 butanol 분획물은 Listeria monocytogen, Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Salmonella typhimurium, Proteus vulgaris, Escherichia coli는 물론 10종의 항생제 내성 병원성 Pseudomonas aeruginosa에 대해서도 우수한 항세균 활성을 나타내었다(최소생육억제농도 1.0~7.5 mg/mL). 또한 ethylacetate 및 butan이 분획물은 일부의 Candida sp.에 대해서도 항균활성을 나타내였다. 한편 n-hexane 분획물을 제외한 산사자 methan이 추출물 및 분획물들은 $500\;{\mu}g/mL$ 농도까지 인간적혈구에 대한 용혈현상을 보이지 않았으며, n-hexane 분획물은 $500\;{\mu}g/mL$ 농도에서 약 9.9%의 미미한 용혈활성을 나타내었다. 이러한 결과는 산사자가 다양한 세균의 제어는 물론 항생제 내성 Pseudomonas aeruginosa 제어를 위한 생물자원으로 개발 기능함을 제시하고 있다.

Keywords

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