Journal of Biosystems Engineering

Korean Society for Agricultural Machinery (한국농업기계학회)
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Detection of Pathogenic Salmonella Using a Surface Plasmon Resonance Biosensor

표면플라즈몬공명 바이오센서를 이용한 살모넬라 검출

  • Cho, Han-Keun (Dept. of Biosystems Engineering, Chungbuk National University) ;
  • Kim, Gi-Young (National Academy of Agricultural Science, RDA) ;
  • Kim, Woon-Ho (Dept. of Biosystems Engineering, Chungbuk National University) ;
  • Sung, Min-Sun (Dept. of Biosystems Engineering, Chungbuk National University)
  • Received : 2010.03.02
  • Accepted : 2010.04.14
  • Published : 2010.04.25
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Abstract

Rapid detection of foodborne pathogens has been a major challenge for the food industry. Salmonella contamination is well known in all foods including pasteurised milk. The possibility of specific detection of Salmonella Enteritidis by surface plasmon resonance (SPR) biosensor was explored using a commercially available portable SPR sensor. Self assembly technique was adopted to immobilize anti-Salmonella antibodies on the gold sensing surface of the SPR sensor. The concentration of polyclonal antibody for use in the SPR biosensor was chosen to 1.0 mg/mL. Experiments were conducted at near real-time with results obtained for one SPR biosensor assay within 1 hour. The limit of detection for Salmonella Enteritidis was determined to be $10^6$ CFU/mL in both PBS buffer and milk samples. The assay sensitivity was not significantly affected by milk matrix. Our results showed that it would be possible for employing the SPR biosensor to detect Salmonella Enteritidis in near real-time.

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References

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