Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Influences of Silkworm Sericin on the Improvement Actions of Lipid Metabolism in Dyslipidemic Rats

실크 세리신이 이상지질혈증 흰쥐의 지질대사 개선작용에 미치는 영향

  • Kim, Han-Soo (Department of Food Science & Technology, Pusan National University) ;
  • Seong, Jong-Hwan (Department of Food Science & Technology, Pusan National University) ;
  • Lee, Young-Guen (Department of Food Science & Technology, Pusan National University) ;
  • Yoon, Ho-Dong (Food & Safety Research Division, National Fisheries Research & Development Institute) ;
  • Kang, Jin-Soon (School of Food Science, International University of Korea) ;
  • Xie, Cheng-Liang (Department of Food Science & Technology, Pusan National University) ;
  • Shin, Ji-Moon (Department of Food Science & Technology, Pusan National University)
  • 김한수 (부산대학교 식품공학과) ;
  • 성종환 (부산대학교 식품공학과) ;
  • 이영근 (부산대학교 식품공학과) ;
  • 윤호동 (국립수산과학원 식품안전과) ;
  • 강진순 (한국국제대학교 식품과학부) ;
  • ;
  • 신지문 (부산대학교 식품공학과)
  • Received : 2010.07.27
  • Accepted : 2010.10.12
  • Published : 2010.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

The principal objective of this study was to investigate the effects of silkworm sericin extract supplementation on the improvement of lipid compositions, blood glucose levels and enzyme activities in the serum of dyslipidemic rats. The rats were fed the experimental diet for 5 weeks. Concentrations of total cholesterol, atherosclerotic index, LDL-cholesterol, triglyceride (TG), phospholipid (PL), free cholesterol, cholesteryl ester ratio and blood glucose in serum were higher in the dyslipidemic group (group HCW) and cholesterol-plus-silkworm sericin extract intake group (group HCS) than those in the control group (group BW, basal diet-plus-water). However, the concentrations of total cholesterol, atherosclerotic index, LDL-cholesterol, TG, PL, free cholesterol, cholesteryl ester ratio and blood glucose level in serum were lower in group HCS than those in group HCW. On the other hand, the HDL-cholesterol level and the ratio of HDL-cholesterol to total cholesterol in group HCS were higher than in group HCW. The activities of alkaline phosphatase (ALP) and aminotransferase (AST & ALT) in serum were lower in group HCS than in the dyslipidemic group HCW. From the above results, it was suggested that silkworm sericin extract intake was effective in the prevention and improvement of lipid components, blood glucose level and enzyme activities in the sera of dyslipidemic rats.

이상지질혈증 흰쥐에 있어서, 누에로부터 효소 처리하여 추출한 세리신의 섭취가 혈청 지질대사 개선효과 및 지질대사 이상 등에 관여하는 지질성분 및 효소의 활성 변동을 검토하기 위하여 이상지질혈증 Sprague Dawley계 흰쥐 수컷을 사용하여 본 실험을 행하였다. 기본식이만을 섭취한 대조군인 BW군을 비롯한 이상지질혈증 유발군(HCW군), 이상지질혈증 유도에 세리신을 급여시킨 군(HCS군)을 5주간 실험 사육하였다. 혈청 총 콜레스테롤, 동맥경화지수, LDL-콜레스테롤, 중성지방, 인지질, 유리 콜레스테롤 농도, 콜레스테롤 에스테르 비 및 혈당 농도 등은 실크 세리신을 급여시킨 군(HCS군)에서 농도가 감소되는 것으로 나타났다. 한편, HDL-콜레스테롤 및 총 콜레스테롤에 대한 HDL-콜레스테롤 비 등은 세리신 섭취(HCS군)에 의해서 증가되는 것으로 나타났다. 혈청 중 alkaline phosphatase (ALP) 및 aminotransferase (AST & ALT)의 활성은 이상지질혈증을 유도 시킨 후 실크 세리신을 섭취시킴으로써 효소 활성이 억제되는 것으로 나타났다. 이상과 같은 결과에서, 실크 세리신이 흰쥐의 혈청 지질대사 이상 등에서 오는 이상지질혈증의 예방 및 개선에 효과가 있을 것으로 생각된다.

Keywords

References

  1. Choi, J. H., D. I. Kim, S. H. Park, J. M. Kim, J. S. Lee, K. G. Lee, J. H. Yeo, and Y. W. Lee. 2000. Effects of silk fibroin on oxidative stress and membrane fluidity in brain of SD rats. Korean J. Life Science 10, 511-518.
  2. Chung, S. H., J. H. Yu, E. J. Kim, and K. S. Ryu. 1996. Blood glucose lowering effect of silkworm. Bull. K. H. Pharma. Sci. 24, 95-100.
  3. Ha, J. S., J. J. Song, H. K. Cho, and S. G. Lee. 2006. Preparation of soluble silk peptides by food-grade proteinases. Korean J. Microbiol. Biotechnol. 31, 115-120.
  4. Hsu, H. C., Y. T. Lee, and M. F. Chen. 2000. Effect of n-3 fatty acids on the composition and binding properties of lipoproteins in hypertriglyceridemic patients. Am. J. Clin. Nutr. 71, 28-35.
  5. Hugli, T. E. and S. Moore. 1972. Determination of the tryptophan content of proteins by ion exchange chromatography of alkaline hydrolysates. J. Biol. Chem. 247, 2828-2832.
  6. Hwang, E. H., B. G. Kang, B. R. Kim, and H. J. Lee. 2001. Protein quality-evaluation and effect of plasma lipid contents of acid hydrolysates of cocoon in rats fed by high cholesterol, high triglyceride and high sucrose diet. J. Korean Soc. Food Sci. Nutr. 30, 1004-1009.
  7. Im, J. G., M. S. Kang, I. K. Park, and S. D. Kim. 2005. Dietary effect of Liriopis tuber water extracts on the level of blood glucose and serum cholesterol in streptozotocin-induced diabetic rat. J. East Asian Soc. Dietary Life 15, 20-28.
  8. Kang, Y. K., S. K. Choo, and S. H. Nam. 2001. The effect of silkworm-extract on blood components levels in mice. Korean J. Entomology 31, 243-247.
  9. Kato, N., S. Sato, A. Yamanaka, H. Yamada, N. Fuwa, and M. Nomura. 1998. Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Biosci. Biotechnol. Biochem. 62, 145-147. https://doi.org/10.1271/bbb.62.145
  10. Kawahara, Y., T. Yamamoto, K. Furukawa, S. Washika, K. Kamei, H. Minami, and S. Nishiuchi. 2007. Sericin hydrolyzate obtained by hydrothermal treatment and its antioxidant activity. SEN'I GAKKAISHI 63, 44-46. https://doi.org/10.2115/fiber.63.44
  11. Kim, J. H. and D. G. Bae. 2003. Hydrolysis characteristics and applications of silk sericin. 1. Control of molecular weight of sericin. Korean J. Seric. Sci. 45, 46-57.
  12. Kim, J. H., D. H. Hahm, D. C. Yang, J. H. Kim, H. J. Lee, and I. S. Shim. 2005. Effect of crude saponin of Korean red ginseng on high-fat diet-induced obesity in the rat. Jpn J. Pharmacol. Sci. 97, 124-131. https://doi.org/10.1254/jphs.FP0040184
  13. Kim, K. H. 1980. A translation: The clinical application of the results of the test. pp. 164-209. Komoonsa, Seoul, Korea.
  14. Kim, K. N., E. S. Joo, K. I. Kim, S. K. Kim, H. P. Yang, and Y. J. Jeon. 2005. Effect of chitosan oligosaccharides on cholesterol level and antioxidant enzyme activities in hypercholesterolemic rat. J. Korean Soc. Food Sci. Nutr. 34, 36-41. https://doi.org/10.3746/jkfn.2005.34.1.036
  15. Kim, S. Y., H. R. Sohn, D. G. Bae, and J. H. Kim. 2003. Physicochemical characteristic of the silkworm sericin cocoon. Korean J. Seric. Sci. 45, 10-17.
  16. Kim, Y. H. 2008. Effect of bread with added silkworm powder and cholesterol on lipid metabolism of rat. Korean J. Food & Nutr. 21, 306-311.
  17. Kinnunen, P. K., J. J. A. Virtanen, and P. Vainio. 1983. Lipoprotein lipase and hepatic endothelial lipase. Atheroscler. Rev. 11, 65-99.
  18. Lee, J. S., H. S. Son, Y. S. Maeng, Y. K. Chang, and J. S. Ju. 1994. Effects of buckwheat on organ weight, glucose and lipid metabolism in streptozotocin-induced diabetic rats. Korean J. Nutrition 28, 819-827.
  19. Lee, J. S., M. H. Lee, H. S. Son, and Y. S. Maeng. 1996. Effects of buckwheat on the activities of pancreatic digestive enzymes in streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 25, 831-838.
  20. Lee, K. G., H. Y. Kweon, Y. W. Lee, J. H. Yeo, S. O. Woo, C. S. Cho, and K. H. Kim. 2002. Characteristics of silk sericin powder prepared by mechanical treatment. Korean J. Seric. Sci. 44, 82-86.
  21. Lee, K. G., J. H. Yeo, Y. M. Lee, H. Y. Kweon, and J. H. Kim. 2001. Bioactive and skin-commpatible properties of silk sericin. Korean J. Seric. Sci. 43, 109-115.
  22. Lee, M. S., D. M. Kim, B. N. Cho, S. J. Koo, S. S. Jew, D. K. Jin, and S. H. Lee. 2003. Study on consequent body fat and serum lipid metabolism after cocoon hydrolysate, green tea leaves and dietary fiber supplementation. J. Korean Soc. Agric. Chem. Biotechnol. 46, 123-129.
  23. Lee, S. H., B. N. Cho, C. K. Hyun, and S. S. Jew. 2002. Physiological, functional characteristics of silk peptide: Antioxidant effect and immune function. Food Science & Industry 35, 57-62.
  24. Lee, S. H., B. N. Cho, I. J. Hong, M. S. Lee, S. S. Jew, D. M. Kim, D. K. Jin, and S. N. Rho. 2003. Effects of green tea, cocoon hydrolysates and capsulated components mixture on body fat and serum lipid composition in college women in terms of supplemented periods. J. Korean Soc. Food Sci. Nutr. 32, 715-722. https://doi.org/10.3746/jkfn.2003.32.5.715
  25. Lee, Y. K., M. Y. Lee, and S. D. Kim. 2004. Quality characteristics and dietary effect of baguette bread added with water extracts of Liriopis tuber on the blood glucose and serum cholesterol in diabetes induced rats. J. East Asian Soc. Dietary Life 14, 275-282.
  26. Lind, L., B. Vessby, and J. Sundstrom. 2006. The apolipoprotcin B/AI ratio and the metabolic syndrome independently predict risk for myocardial infarction in middle-aged men. Arterioscler. Thromb. Vasc. Biol. 26, 406-410. https://doi.org/10.1161/01.ATV.0000197827.12431.d0
  27. Mochizuki, H., J. Takido, H. Oda, and H. Yokogoshi. 1999. Improving effect of dietary taurine on marked hypercholesterolemia induced by a high-cholesterol diet in streptozotocin-induced diabetic rats. Biosci. Biotechnol. Biochem. 63, 1984-1987. https://doi.org/10.1271/bbb.63.1984
  28. Nam, J. K. and Y. S. Oh. 1995. A study of pharmachological effect of silk fibroin RDA. Korean J. Agric. Sci. 37, 145-157.
  29. No, H. K., K. Y. Beik, and S. J. Kim. 2002. Effect of chitosan-soybean curd on serum lipid metabolism in rats fed high-fat diet. J. Korean Soc. Food Sci. Nutr. 31, 1078-1083. https://doi.org/10.3746/jkfn.2002.31.6.1078
  30. Park, I. K., H. S. Lee, K. Y. Lee, and Y. J. Ahn. 1988. Cytotoxic activity of Bombyx mori and Morus alba derived materials against human tumor cell lines. Agric. Chem. Biotech. 41, 187-190.
  31. Razdan, A. and D. Pettersson. 1994. Effect of chitosan on nutrient digestibility and plasma lipid concentration in broiler chickens. Br. J. Nutr. 72, 277-288. https://doi.org/10.1079/BJN19940029
  32. Reitman, S. and S. Frankel. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am. J. Clin. Pathol. 28, 56.
  33. Smith, E. B. 1974. The relationship between plasma and tissue lipid in human atherosclerosis. Adv. Lipid Res. 11, 1-7.
  34. Yeo, J. H., K. G. Lee, S. O. Woo, H. Y. Kweon, S. M. Han, K. H. Park, and S. S. Kim. 2007. Dietary effect of silk protein on colon cancer of animal. Korean J. Seric. Sci. 49, 67-70.
  35. Yi, K. N., and C. S. Rhee. 1996. Clinical Pathology File. pp. 101-283. Euihakmunwhasa, Soeul, Korea.
  36. Yoon, J. W., S. K. Rhee, and K. B. Lee. 2005. Effects of silkworm extract powder on plasma lipids and glucose in rats. Korean J. Food Nutr. 18, 140-145.

Cited by

  1. Improvements Caused by Chitosan, Sericin and Collagen Peptide Extract Complexes on Lipid Metabolism in Dyslipidemia vol.20, pp.8, 2011, https://doi.org/10.5322/JES.2011.20.8.1021
  2. Influences of Chitosan, Sericin and Collagen Extract Complexes on the Improvement Actions of Lipid Component in Diabetes vol.20, pp.8, 2011, https://doi.org/10.5322/JES.2011.20.8.1031