Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

Ginsenoside Contents and Antioxidative Activities from Red Ginseng Treated with High Hydrostatic Pressure

초고압 처리한 홍삼의 ginsenoside 함량 및 항산화 활성

  • Shin, Chang-Sik (Nutrex Technology R&D center) ;
  • Lee, Do-Hyun (Nutrex Technology R&D center) ;
  • Kim, Sung-Han (Nutrex Technology R&D center) ;
  • Shin, Min-Ho (Dept. of Food Science and Biotechnology, Kunsan Nat'l Univ.) ;
  • Jeong, Chang-Ho (Dept. of Food Sci. and Techn., Gyeongsang Nat'l Univ. (Insti. of Agric. & Life Sci.)) ;
  • Shim, Ki-Hwan (Dept. of Food Sci. and Techn., Gyeongsang Nat'l Univ. (Insti. of Agric. & Life Sci.))
  • 신창식 (뉴트렉스테크놀로지 생명공학연구소) ;
  • 이도현 (뉴트렉스테크놀로지 생명공학연구소) ;
  • 김성한 (뉴트렉스테크놀로지 생명공학연구소) ;
  • 신민호 (군산대학교 식품생명공학과) ;
  • 정창호 (경상대학교 식품공학과(농업생명과학연구원)) ;
  • 심기환 (경상대학교 식품공학과(농업생명과학연구원))
  • Received : 2010.09.24
  • Accepted : 2010.12.17
  • Published : 2010.12.31
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Abstract

This study was carried out to investigate the changes of ginsenoside contents and antioxidative activities from red ginseng after treated with high hydrostatic pressure (RGHHP). Crude saponin content in traditional red ginseng (TRG) and RGHHP were 21.93 and 27.29 mg/g, respectively. The contents of total phenolics, crude saponin and ginsenoside increased after treated with high hydrostatic pressure. TRG and RGHHP showed an increase 25.60% the highest content of Rb1 (14.10 mg/g and 17.71 mg/g). Also, Rg3 content compared with TRG and RGHHP increased 10.46%. The radical scavenging activity of hot water extract from red ginseng against the DPPH and ABTS radicals increased with the increasing amount of extract and RGHHP higher than TRG. The reducing power and ferric reducing antioxidant power (FRAP) assays of the red ginseng were increased in a dose dependent manner. The FRAP of TRG and RGHHP were 0.30 and 0.36 absorbance, respectively at a concentration of 10 mg/mL. The present results suggest that RGHHP would have the protective potential from oxidative stress induced by free radicals.

초고압 처리가 홍삼의 진세노사이드 함량 및 항산화 활성에 미치는 영향에 대하여 조사하였다. 전통적인 방법으로 제조한 일반 홍삼과 초고압 처리 홍삼의 조사포닌 함량은 각각 21.93 및 27.29 mg/g이었고, 총 페놀성 화합물과 조사포닌 및 진세노사이드 함량은 초고압 처리에 의해 증가하였다. 일반 홍삼과 초고압 처리 홍삼에 가장 많이 함유되어 있는 진세노사이드는 Rb1으로 각각 14.10 mg/g과 17.71 mg/g 이었으며, 초고압 처리에 의하여 25.60% Rb1이 증가하였다. 또한 홍삼의 특징적인 유효 성분인 Rg3는 일반 홍삼에 비하여 초고압 처리한 홍삼에서 10.46% 증가하였다. DPPH 및 ABTS 라디칼 소거 활성을 측정한 결과, 열수 추출물의 농도가 증가함에 따라 DPPH 및 ABTS 라디칼 소거 활성이 증가하는 경향을 보였으며 초고압 처리 홍삼이 일반 홍삼 열수 추출물에 비해 높은 라디칼 소거 활성을 보였다. 환원력과 FRAP 활성도 분획물의 농도가 증가함에 따라 환원력과 FRAP 활성이 증가하는 경향을 보였으며, 일반 홍삼과 초고압 처리 홍삼 열수 추출물의 FRAP 활성은 농도 10 mg/mL일 때 각각 0.30 및 0.36의 흡광도를 보였다. 따라서 초고압 처리 홍삼 열수 추출물은 자유 라디칼에 의해 유발된 산화적 스트레스를 효과적으로 방어할 수 있을 것으로 판단된다.

Keywords

Acknowledgement

Grant : 초고압 기술을 이용한 Global 홍삼제품 연구 개발

Supported by : 지식경제부

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