Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Functionality Analysis of Korean Medicine Fermented by Lactobacillus Strains

유산발효에 의한 발효한약의 기능분석

  • Kang, Dong-Hee (Department of Microbiology, College of Natural Science, Keimyung University) ;
  • Kim, Hyun-Soo (Department of Microbiology, College of Natural Science, Keimyung University)
  • 강동희 (계명대학교 자연과학대학 미생물학과) ;
  • 김현수 (계명대학교 자연과학대학 미생물학과)
  • Received : 2011.02.01
  • Accepted : 2011.06.16
  • Published : 2011.09.28
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Abstract

Through the process of fermentation with Lactobacillus strains this study has evaluated the functionality of the traditional Korean medicine Bangpungtongsungsan after the addition of four other medicinal ingredients. In order to facilitate the growth of the Lactobacillus strains brown sugar was added to the herbal substances used. For both DPPH radical scavenging activities and SOD-like activities the medicinal mixture, when fermented through heterogeneous co-cultures, scored higher (at 77% and 42%, respectively) than when not fermented (at 31.7% and 36.3%, respectively). The co-cultured Korean medicine inhibited the growth of Bacillus subtilis PCI 219, Pseudomonas aeruginosa KCTC 2004, Staphylococcus aureus subsp. aureus KCTC 1916 and Propionibacterium acnes KCTC 3314. The inhibiting effects on ${\beta}$-hexosaminidase released from RBL-2H3 cells caused by the mixture, with and without fermentation, was seen to be similar (57% and 60%, respectively).

본 연구에서는 중풍, 고혈압, 동맥경화, 염증성 피부질환에 사용되고 있으며, 다양한 질환들에서 연구되어 있는 방풍통성산을 선정하여 4가지 약재를 가미한 후 기존의 기능성 물질과 Lactobacillus 균주에 의해 생성되거나 증가된 기능성 물질을 확인하고자 하였다. 한약을 이용한 복합배양은 공시균주의 수 및 접종시기를 달리하여 여러 조합으로 수행하였다. 전자공여능 및 superoxide dismutase 유사 활성 측정을 통해 한약의 항산화 활성은 각각 31.7%와 36.3%로 나타났으며, 복합배양을 통해 발효된 한약의 항산화 활성은 각각 77% 이상, 42% 이상으로 나타나 한약보다 항산화 활성이 더 높게 나타났다. 항균 활성은 한약의 경우 모든 시험균주에 항균 활성이 나타나지 않았으나, 복합배양 된 한약의 경우에는 B. subtilis PCI 219, P. aeruginosa KCTC 2004, S. aureus subsp. aureus KCTC 1916에 항균 활성을 보였으며, 특히 여드름을 유발시키는 P. acnes KCTC 3314에 항균 활성을 보였다. RBL-2H3 세포주에서 알레르기 억제 효과를 확인한 결과 한약은 60% 알레르기 억제 효과를 보였으며, 발효한약은 57% 알레르기 억제 효과를 보였다. 발효한약은 알레르기 억제 효과와 함께 항균 활성과 항산화 활성의 증가로 인하여 알레르기 개선, 피부노화 및 병원성 미생물에 의한 피부질환의 개선에 효과가 있을 것으로 사료된다.

Keywords

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