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Quality Properties of Fermented Squid Seasoning Manufactured with Fermentation Accelerator

발효촉진제를 첨가하여 제조한 오징어 조미료의 품질특성

  • Choi, Seung-Hwa (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Kim, Sang-Moo (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
  • 최승화 (강릉원주대학교 해양식품공학과) ;
  • 김상무 (강릉원주대학교 해양식품공학과)
  • Received : 2011.02.08
  • Accepted : 2011.04.14
  • Published : 2011.06.30

Abstract

Squid was fermented with fermentation accelerators to develop a natural complex seasoning. The quality properties of fermented squid were determined at different fermentation periods. Squid fermented with 10% Aspergillus oryzae koji for 10 days had the highest amino-N, acidity, and total viable cell content during fermentation periods, whereas volatile basic nitrogen content and pH were the lowest. Based on the amino-N content, squid with 10% koji fermented for 10 days was selected for further analyses. The inosine and glutamic acid contents of the fermented squid were highest innucleotide composition, their related compounds, and free amino acids, respectively. The $IC_{50}$ values of the fermented squid on DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and ${\alpha}$-glucosidase inhibitory activities were 6.20 and 4.41 mg/mL, respectively. Based on the results of a sensory evaluation, the fermented squid seasoning was similar to other natural complex seasonings such as anchovy, cowmeat, and fisheries seasonings.

본 연구는 오징어 육에 발효촉진제를 첨가하여 발효한 오징어 조미료 소재 개발을 목적하며 결과를 요약하면 다음과 같다. Asp. oryzae koji를 10%첨가하여 발효하였을 때 아미노질소량이 가장 높았고 휘발성 염기질소량이 가장 낮았으며, pH의 감소량이 크고 산도가 높아 발효에 가장 알맞은 조건이었으며, 10일간 발효하였을 때 아미노질소량이 가장 높아 발효생성물을 제조하기에 가장 적합하였다. Asp. oryzae koji 10%의 농도에 10일간 발효한 오징어 육 발효생성물의 유리 아미노산 조성은 감칠맛을 내는 아미노산인 glutamic acid의 함량이 가장 많았고 핵산관련물질 중 감칠맛을 내는 IMP가 쓴맛을 내는 hypoxanthine보다 많아 식품 정미소재로 이용하기에 적합하였다. 항산화 및 ${\alpha}$-glucosidase 저해활성은 농도 의존적으로 증가하였으며, 항산화 및 소장에서 단당류의 생성을 억제하여 혈당을 낮출 수 있는 가능성이 있는 기능성 소재로서의 활용가능성도 있다고 보여진다. 오징어 발효생 성물에 부재료를 첨가하여 조미료를 제조한 결과 시판 천연조미료와 비교하여 관능적으로 큰 차이가 없었다. 이상의 결과를 고려할 때 오징어 육 발효생성물은 기능성 소재로의 활용에 대하여는 추가연구가 필요하지만, 천연조미료 등 식품 정미 소재로써 활용이 가능하다고 판단된다.

Keywords

References

  1. Kim JH, Lee YM, Joo NM, Choi KS, Sohn CM, Park SH, Chung CS, Do HJ, Ryou HJ. Development and application of a novel tomato sauce using natural seasoning. Korean J. Food Cookery Sci. 26: 138-145 (2010)
  2. Kim SK, Byun HG, Jeon YJ, Joo DS, Kim JB. Deveolopement of natural seasoning using desalinated tuna boiled extract. J. Korean Fish Soc. 32: 75-82 (1999)
  3. Kim EJ, Cha YJ. Development of functional seasoning agents from skipjack preparation by- product with commercial protease: Processing of hydrolysate from skipjack processing by product with protease treatment. J. Korean Soc. Food Nutr. 25: 608-616 (1996)
  4. Cho SY, Joo DS, Park SH, Kang HJ, Jeon JK. Change of taurine content in squid meat during squid processing and taurine content in the squid processing waste water. J. Korean Fish Soc. 33: 51- 54 (2000)
  5. Kwon MC, Qadir SA, Kim HS, Ahn JH, Cho NH, Lee HY. UV protection and whitening effects of collagen isolated from outer layer of the squid Todarodes pacificus. J. Korean Fish Soc. 41: 7- 12 (2008)
  6. Lee JS, Kim YS. Baking characteristics of taurine supplemented bread and cookies and its effect on blood alcohol concentrations. Korean J. Food. Nutr. 22: 479-484 (2009)
  7. Jeon YS, Kang SM. Influence of collagen intake upon facial-skin dermis and pigmentation. J. Korean Soc. Cosm. 15: 745-756 (2009)
  8. MFAFF. Food, Agriculture, Forestry and Fisheries Statistical Yearbook. Ministry for Food, Agriculture, Forestry and Fisheries, Seoul, Korea. pp. 280-317 (2009)
  9. Lee SM, Lim YS, Lee JK, Park SR. Effects of supplemental squid meal, attractant, herb of lecithin in the formulated diets on growth performance in juvenile abalone (Haliotis discushannai). J. Korean Fish Soc. 32: 290-294 (1999)
  10. Nam KA, You SG, Kim SM. Molecular and physical characteristics of squid (Todarodes pacificus) skin collagens and biological properties of their enzymatic hydro-lysates. J. Food Sci. 73: 249- 255 (2008) https://doi.org/10.1111/j.1750-3841.2008.00722.x
  11. Seo SH, Jeong YJ. Quality characteristics for doenjang using squid internal organs. Korean J. Food Sci. Technol. 33: 89-93 (2001)
  12. Kim HS, Kim JS, Heu MS. Fractionation of endoprotease from viscera of the argentina shortfin squid Illex argentinus. J. Korean Fish Soc. 41: 176-181 (2008)
  13. Choi GP, Kim SM, Shin IS. Antimicrobial activity of squid pen $\beta$-chitosan. J Chitin Chitosan 8: 149-155 (2003)
  14. Kim YM, Koo JG, Lee CY, Kim DS. Study on the use of sardine meal koji and autolysates from sardine meat in rapid processing of sardine sauce. Bull. Korean Fish Soc. 23: 169-177 (1990)
  15. Lee GG, Lee HD, Lee CH. Changes in sensory characteristics during salt aging of doenjang (fermented soybean paste) made by different starters. Food Eng. Prog. 7: 13-19 (2003)
  16. Oh HI, Eom SM. Changes in microflora and enzyme activities of cheonggukjang prepared with germinated soybeans during fermentation. Korean J. Food Sci. Technol. 40: 56-62 (2008)
  17. Pyeun JH, Heu MS, Cho DM, Kim HR. Proteolytic properties of cathepsin L, chymotrypsin and trypsin from the muscle and viscera of anchovy, Engraulis japonica. J. Korean Fish Soc. 28: 557-568 (1995)
  18. Pyeun JH, Lee DS, Kim DS, Heu MS. Activity screening of the proteolytic enzymes responsible for post-mortem degradation of fish tissues. J. Korean Fish Soc. 29: 296-308 (1996)
  19. Kim WJ, Kim SM. The chemical and microbial characteristics of northern sand lance, Ammodytes personatus, sauce manufactured with fermentation accelerating agents. Korean J. Food Sci. Technol. 35: 447-454 (2003)
  20. Nissen JA. Determination of the degree of hydrolysis of food protein hydrolysates by trinitrobenzenesulfonic acid. J. Agr. Food Chem. 27: 1256-1262 (1979) https://doi.org/10.1021/jf60226a042
  21. Chae SK. Standard Food Analysis. Jiju Publishing Co., Seoul, Korea. pp. 637-640 (1998)
  22. AOAC. Official Methods of Analysis. 11th ed. Method 986.25 Associ-ation of Official Analytical Chemists, Washington, DC, USA (2002)
  23. KFDA. Food Code. Korea Food and Drug Administration, Seoul, Korea. pp. 94-104 (2000)
  24. Ryu KY, Shim SL, Kim W, Jung MS, Hwang IM, Kim JH, Hong CH, Jung CH, Kim KS. Analysis of the seasonal change of the proximate composition and taste components in the conger eels (Conger myriaster). J. Korean Soc. Food Sci. Nutr. 38: 1069- 1075 (2009) https://doi.org/10.3746/jkfn.2009.38.8.1069
  25. Blois MS. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200 (1958) https://doi.org/10.1038/1811199a0
  26. Kim KY, Nam KA, Kurihara H, Kim SM. Potent $\alpha$-glucosidase inhibitors purified from the red alga Grateloupia elliptica. Phytochemistry 69: 2820-2825 (2008) https://doi.org/10.1016/j.phytochem.2008.09.007
  27. Lee WJ, Jung JK. Quality characteristics and preparation of noodles from brown rice flour and colored rice flour. Korean J. Culinary Res. 8: 267-278 (2002)
  28. Park JH, Kim SM. Property changes of the salt-sea-soned and fermented the broken roes of alaska pollock stuffed into cellulose casing. Korean J. Food Sci. Technol. 34: 220-224 (2002)
  29. Kim SM, Baek OD, Lee KT. The development of squid (Todarodes pacificus) sikhae in Kangnung district: 4. The effects of red pepper and grain contents on theproperties of squid sikhae. Bull. Korean Fish Soc. 27: 366-372 (1994)
  30. Cha YJ, Kim SJ, Keong EJ, Kim H, Cho WJ, Yoo MY. Studies on taste compounds in alaska pollack sikhae during fermentation. J. Korean Soc. Food Sci. Nutr. 33: 1515-1521 (2004) https://doi.org/10.3746/jkfn.2004.33.9.1515
  31. Oh SC, Cho JS, Nam HY. Changes of the volatile basic nitrogen and free amino acids according to the fermentation of law salt fermented squid. Korean J. Soc. Food Sci. 16: 173-181 (2000)
  32. Cha YJ, Park HS, Cho SY, Lee EH. Studies on the processing of low salt fermented sea foods: 4. Processing of low salt fermented anchovy. Bull. Korean Fish Soc. 16: 363-367 (1983)
  33. Lee WD, Chang DS, Koh BH, Lee MS, Jeong ET. Quality analysis of viscera of alaska pollack treated on vessel for raw materials of changranjeotgal. J. Korean Fish. Soc. 30: 271-276 (1997)
  34. Lee JE, Lee SY. Growth characteristics of Bifidobacteria and quality characteristics of soy yogurt prepared with different proteolytic enzymes and starter culture. Korean J. Food Sci. Technol. 33: 603-610 (2001)
  35. Kim YA, Kang ST, Kang JG, Kang JY, Yoo UH, Oh KS. Processing and quality characteristics of law-salt fermented ascidian Halocynthia roretzi. J. Korean Fish Soc. 39: 283-291 (2006)
  36. Koo JG, Yoo JH, Park KS, Kim SY. Biochemical and microbiological changes of hard clam shikhae during fermentation. Korean J. Fish Aquat. Sci. 42: 569-573 (2009) https://doi.org/10.5657/kfas.2009.42.6.569
  37. Kim SM, Cho YJ, Lee KT. The development of squid (Todarodes pacificus) sikhae in Kangnung district: 2. The effect of fermentation temperatures and periods on chemical and microbial changes and the partial purification of protease. Bull. Korean Fish Soc. 27: 223-231 (1994)
  38. Oh KH, Lee KE, Kim JM, Lee SC. Characteristics of whelk internal organ jeotgal with the addition of bromelain. Korean J. Food Sci. Technol. 33: 78-83 (2001)
  39. Son DH, Kwon OJ, Choi UK, Kwon OJ, Lee SI, Im MH, Kwon KI, Kim SH, Chung YG. Taste characteristics of ganjang made with barley bran. J. Korean Soc. Agric. Chem. Biotechnol. 45: 18-24 (2002)
  40. Kim SK, Baek HC, Hyun HG, Kang OJ, Kim JB. Biochemical composition and antioxidative activity of marine microalgae. J. Korean Fish Soc. 34: 260-267 (2001)
  41. Nakamura K, Shikma SI, Kimoto K, Mizuno Y. Change in freshness of Japanese common squid during cold storage. Bull. Tokai Reg. Fish Res. Lab. 118: 45-49 (1985)
  42. Sheih IC, Wub TK, Fang TJ. Antioxidant properties of a new antioxidative peptide from algae protein waste hydrolysate in different oxidation systems. Bioresource Technol. 100: 3419-3425 (2009) https://doi.org/10.1016/j.biortech.2009.02.014
  43. Toller M. $\alpha$-Glucosidase inhibitors in diabetes: Efficacy in NIDDM subjects. Eur. J. Clin. Invest. 24: 31-35 (1994)

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