Korean journal of food and cookery science (한국식품조리과학회지)

Korean Society of Food and Cookery Science (한국식품조리과학회)
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Antimicrobial Activity against Food Hazardous Microorganisms and Antimutagenicity against Salmonella serotype Typhimurium TA100 of an Ethanol Extract from Sanguisorba officinalis L.

지유 에탄올 추출물의 식품 위해성 세균에 대한 항균 활성 및 Salmonella serotype Typhimurium TA100에 대한 항돌연변이 활성 효과

  • Kim, Se-Ryoung (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Won, Ji-Hye (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Mee-Ra (Dept. of Food Science and Nutrition, Kyungpook National University.Center for Beautiful Aging)
  • 김세령 (경북대학교 식품영양학과) ;
  • 원지혜 (경북대학교 식품영양학과) ;
  • 김미라 (경북대학교 식품영양학과.장수생활과학연구소)
  • Published : 2011.08.30
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Abstract

This study was performed to analyze the antibacterial activity against food hazardous microorganisms and antimutagenic effects of Sanguisorba officinalis L. ethanol extracts on Salmonella Typhimurium TA100. The antibacterial activity was evaluated by paper disc diffusion assay, minimum inhibition concentration (MIC), and optical density of the culture with the ethanol extract for 24 hr. Antibacterial activity was tested with seven microorganisms including Escherichia coli, Escherichia coli O157:H7, Pseudomonas aeruginosa, Salmonella Typhimurium, Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus. The paper disc diffusion assay showed distinct clear inhibition zones around the discs treated with the extract for five microorganisms, except Escherichia coli and Escherichia coli O157:H7. MIC values were 0.625-2.5 mg/mL for these five strains that showed clear zones. The time-kill assay was consistent with the results from the paper disc diffusion assay and MIC test. Additionally, antimutagenicity of the extract was determined using the Ames test. The ethanol extract at 5 mg/plate inhibited 72.42% and 89.85% of mutagenicity induced by 4-nitroquinoline 1-oxide and sodium azide, respectively. These results demonstrate that the ethanol extract from S. officinalis L. has remarkable antibacterial activity and antimutagenicity.

본 연구는 지유 에탄올 추출물의 항균 및 항돌연변이 활성을 측정하여 새로운 기능성 소재로서의 기초자료를 얻고자 실시하였다. E. coli, E. coli O157:H7, P. aeruginosa, S. Typhimurium, L. monocytogenes, B. cereus, S. aureus의 7가지 주요 식품위해성 세균에 대한 항균활성을 검증하기 위해 paper disc diffusion assay 및 최소저해농도를 측정하였으며 24시간 동안 균의 생육억제도를 측정하였다. Paper disc assay 결과 E. coli와 E. coli O157:H7을 제외한 5가지 균에 대한 지유 에탄올 추출물의 뚜렷한 clear zone이 확인되었다. Micro-well dilution법으로 확인한 최소저해농도의 범위는 E. coli와 E. coli O157:H7을 제외한 5가지 균에 대해서 0.625~2.5 mg/mL로 나타났으며 생육억제도 실험결과도 최소 저해농도 결과와 일치하였다. Salmonella Typhimurium TA100을 이용한 Ames test 결과, 5 mg/plate의 농도에서 직접 돌연변이원인 sodium azide와 4-NQO에 대한 지유 에탄올 추출물의 항돌연변이 활성이 각각 72.42와 89.85%로 나타나 지유 에탄올 추출물의 우수한 항돌연변이 활성을 확인하였다. 본 연구를 통해 지유 에탄올 추출물이 주요 식품위해성 세균들에 대한 우수한 항균성과 직접돌연변이원에 대한 높은 항돌연변이 활성을 나타내어 천연물 유래 생리활성 소재로 이용될 수 있는 가능성을 보여주었다.

Keywords

References

  1. 서부일. 2004. 알기 쉬운 본초학. 대구한의대학교출판부 pp 267
  2. 임종필. 2003. 본초생화학. 신일상사. 서울 pp 247
  3. 한국생약학교수협의회. 2002. 본초학. 아카데미서적. 서울 pp 488
  4. 한국한의과 본초학 편찬위원회. 2004. 본초학. 영림사. 서울 pp 437-438
  5. Ahn DJ, Kwak YS, Kim MJ, Lee JC, Shin CS Jeong KT. 2000. Screening of herbal plant extracts showing antimicrobial activity against some food spoilage and pathogenic microorganisms. Korean J Medicinal Crop Sci 8(2):109-116
  6. Ahn EY, Shin DH, Baek NI Oh JA. 1998. Isolation and identification of antimicrobial active substance from Sophora flavescens Ait. Korean J Food Sci Technol 30(3):672-679
  7. An BJ, Lee JT, Lee SA, Kwak JH, Park JM, Lee JY Son JH. 2004a. Antioxidant effects and application as natural ingredients of Korean Sanguisorbae officinalis L. J Korean Soc Appl Biol Chem 47(2):244-250
  8. An BJ, Lee JT, Park JM, Kwak JH, Lee JY, Park CI, Son JH, Im HY. 2003. Isolation of novel glucosyltransferase and tyrosinase inhibitor from the roots of korean Sanguisorbae officinalis L. Kor J Herbology 18(4):255-262
  9. An BJ, Lee SA, Son JH, Kwak JH, Park JM, Lee JY. 2004b. Cytotoxic and antibacterial activities of Sanguisorbae officinalis L. J Korean Soc Appl Biol Chem 47(1):141-145
  10. Cha JY, Ha SE, Sim SM, Park JK, Chung YO, Kim HJ, Park NB. 2008. Antimicrobial effects of ethanol extracts of Korean endemic herb plants. Korean J Life Sci 18(2):228-233 https://doi.org/10.5352/JLS.2008.18.2.228
  11. Chu SM. 2007. Antioxidative and antimicrobial activities of medicinal plants collected from Nepal. M.A. Thesis. Dankook University. Korea pp 22-26
  12. Chung DH, Beak SH. 2003. Antibacterial activities of honeys on the Staphylococcus aureus. Korean J Food & Nutr 15(2):158-164
  13. Chung SH, Yoon KH. 2008. Antimicrobial activity of extracts and fractions of green tea used for coarse tea. J Korean Soc Food Sci Nutr 37(11):1382-1388 https://doi.org/10.3746/jkfn.2008.37.11.1382
  14. Demirci F, Guven K, Demirci B, Dadandi MY, Baser KHC. 2008. Antibacterial activity of two Phlomis essential oils against food pathogens. Food Control 19(12):1159-1164 https://doi.org/10.1016/j.foodcont.2008.01.001
  15. Han JS, Shin DH. 1994. Antimicrobial effect of each solvent fraction of Morus alba linne, Sophora flavescens AITON on Listeria monocytogenes. Korean J Food Sci Technol 26(5):539-544
  16. Han WS. 2004. Isolation of antimicrobial compounds from Salvia miltiorrhiza bunge. Korean J Medicinal Crop Sci 12(3): 179-182
  17. Jeon YH, Choi SW, Kim MR. 2009. Antimutagenic and cytotoxic activity of ethanol and water extracts from Rubus coreanum. Korean J Food Cookery Sci 25(3): 379-386
  18. Jeon YH, Kil JH, Lim SM, Kim MH, Kim MR. 2008a. Analysis of antioxidative activity and antimutagenic effect of ethanol extract from Schizandra chinensis Baillon. J East Asian Soc Dietary Life 18(5): 746-752
  19. Jeon YH, Kim MH, Kim MR. 2008b. Antioxidative and antimutagenic activity of ethanol extracts from Cuscutae semen. Korean J Food Cookery Sci 24(1): 46-51
  20. Jeon YH, Kim MH, Kim MR. 2008c. Antioxidative, antimutagenic, and cytotoxic activities of ethanol extracts from Cornus officianalis. J Korean Soc Food Sci Nutr 37(1): 1-7 https://doi.org/10.3746/jkfn.2008.37.1.1
  21. Jun HS, Sung MK. 1999. Effect of soybean saponins in aflatoxin B1-induced mutagenicity. Korean J Nutr 32(1): 110-117
  22. Kang SY. 2005. The antimicrobial compound of Rhus verniciflua barks against fish pathogenic gram-negative bacteria, Edwardsiella tarda and Vibrio anguillarum. J Fish Pathol 18(3): 227-237
  23. Kim HK. Kim YA, Hwang SW, Ko BS. 2002. Quantitative analysis of ziyuglycoside I from Sanguisorbae radix. Kor J Pharmacogn 33(2): 100-104
  24. Kim JG, Kang YM, Eum GS, Ko YM, Kim TY. 2003. Antioxidative activity and antimicrobial activity of extracts from medicinal plants(Akebia quinate Decaisn, Scirusfluviatilis A. Gray, Gardenia jasminoides for. grandiflora Makino). J Agric Life Sci 37(4): 69-75
  25. Kim KJ, Do JR, Kim HK. 2005. Antimicrobial, antihypertensive and anticancer activities of garlic extracts. Korean J Food Sci Technol 37(2): 228-232
  26. Kim SA, Kim J, Woo MK, Kwak CS, Lee MS. 2005. Antimutagenic and cytotoxic effects of ethanol extracts from five kinds of seaweeds. J Korean Soc Food Sci Nutr 34(4): 451-459 https://doi.org/10.3746/jkfn.2005.34.4.451
  27. Kim TG, Park MS, Han HM, Kang SY, Jung KK, Rheu HM, Kim SH. 1999. Inhibitory effects of Terminalia chebula, Sanguisorba officinalis, Rubus coreanus and Rheum palmatum on hepatitis B virus replication in HepG2 2.2.15 Cells. Yakhak Hoeji 43(4): 458-463
  28. Kim TY, Jeon TW, Yeo SH, Kim SB, Kim JS, Kwak JS. 2010. Antimicrobial, antioxidant and SOD-like activity effect of Jubak extracts. Korean J. Food & Nutr 23(3): 299-305
  29. Kim YS, Roh JE, Ann HS. 1993. Compositional analysis of polysaccharide from Sanguisorba officinalis and its anticoagulant activity. Kor J Pharmacogn 24(2): 124-130
  30. Kokoska L, Polesny Z, Rada V, Nepovim A, Vanek T. 2002. Screening of some Siberian medicinal plants for antimicrobial activity. J Ethnopharmacology. 82(1): 51-53 https://doi.org/10.1016/S0378-8741(02)00143-5
  31. Lee DJ, Bae RN, Kim HW, Chu SM, Park SY, Lee JS. 2007. Antioxidant and antimicrobial activities of herbs and spices. Korean J Intl Agric 19(1): 38-42
  32. Lee JJ, Choi HS, Lee JH, Jung CJ, Lee MY. 2005. The effects of ethylacetate fraction of Sanguisorba officinalis L. on experimentally-induced acute gastritis and peptic ulcers in rats. J Korean Soc Food Sci Nutr 34(10): 1545-1552 https://doi.org/10.3746/jkfn.2005.34.10.1545
  33. Lee KT, Lee YK, Lee JP, Lee JW, Son SK, Choi SH, Lee SB. 2007. Determination of the prevalence of pathogenic bacteria and the changes in microbiological growth pattern of cured and short-ripened raw ham during storage. Korean J. Food Sci. Ani. Resour. 27(1): 127-131 https://doi.org/10.5851/kosfa.2007.27.1.127
  34. Lee NJ, Lee KH, Lee DU, Park SS, Han YH, Ryu SY. 2001. Antimutagenic activity and cytotoxicity of the whole plant of Rumex acetosa. Kor J Pharmacogn 32(4): 338-343
  35. Lee SY, Chung SH. 1994. Anxiolytic activities of Sanguisorbae radix. Yakhak Hoeji 38(6): 733-741
  36. Madigan MT, Martinko JM 2006. Brock biology of microorganisms. 12th ed. seoul. Worldscience. pp 107-114
  37. Maron MI, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutat. Res 113: 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  38. Mikaki Y, Fukushima M, Yokosuka A, Sashida Y, Furuya S, Sakagami H. 2001. Triterpene glycoside from the roots Sanguisorba officinals. Phytochemistry 57(5): 773-779 https://doi.org/10.1016/S0031-9422(01)00083-8
  39. Mok JS, Park UY, Kim YM, Chang DS. 1994. Effects of solvents and extracting condition on the antimicrobial activity of Salviae miltiorrhizae radix(Salvia miltiorrhiza) extract. J Korean Sco Food Nutr 23(6): 1001-1007
  40. Oh SI, Lee MS. 2007. Antioxidative stress and antimutagenic effects of Lentinus edodes ethanol extracts. Korean J Food & Nutr 20(4): 341-348
  41. Park CS, Choi MA, Park GS, Choi KH. 2002. Antibacterial activities of Cordyceps spp., Mugwort and Pine needle extracts. Korean J Food Preserv 9(1): 102-108
  42. Park JS, Ahn BY, Koh HY, Choi DS. 2003. Inhibitory effect of methanol extract of Eeutherococcus senticosus maxim on the direct mutagen mutagenicity. Korean J Biotechnol Bioeng 18(3): 217-221
  43. Park SW, Kim SH, Chung SK. 1995. Antimutagenic effects and isolation of flavonoids from Humulus japonicus extract. Korean J Food Sci Technol 27(6): 897-901
  44. Park SW, Woo CJ, Chung SK, Chung KT. 1994. Antimicrobial and antioxidative activities of solvent fraction from Humulus japonicus. Korean J Food Sci Technol 26(4): 464-470
  45. Park YO, Kim HS. 2010. Antimicrobial activity of bamboo (Sasa borealis) leaves fraction extracts against food poisoning bacteria. J Korean Soc Food Sci Nutr 39(13): 1745-1752 https://doi.org/10.3746/jkfn.2010.39.12.1745
  46. Ryu BH, Lee BH, Ha MS, Kim DS, Sin DB, Nam KD. 1986. Desmutagenic effect of legumes and plants crude saponins in Salmonella typhimurium TA 98. Korean J. Food Sci. Technol 18(5): 345-350
  47. Seon HJ, Joo IS, Sung CK. 1999. A study on suppression components of spoiling Ginseng. J Ginseng Res 23(2): 67-73
  48. Son DH, Lee SI, Chung YG. 2001. Antioxidative of medicinal plants on pathogenic bacteria. J Korean Soc. Hygienic Sciences 7(2): 103-108
  49. Yi SD, Yang JS, Jeong JH, Sung CK, Oh MJ. 1999. Antimicrobial activities of Soybean paste extracts. J Korean Soc Food Sci Nutr 28(6): 1230-1238
  50. Yoo MY, Jung YJ, Yang JY. 2005. Antimicrobial activity of herb extracts. J Korean Soc Food Sci Nutr 34(8): 1130-1135 https://doi.org/10.3746/jkfn.2005.34.8.1130

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