KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Effect of Particle Size on Antioxidant Activity and Cytotoxicity in Purple Corn Seed Powder

검정찰옥수수 종실 분쇄 정도에 따른 항산화 및 Cytotoxicity 활성 효과

  • 김정태 (농촌진흥청 국립식량과학원) ;
  • 손범영 (농촌진흥청 국립식량과학원) ;
  • 이진석 (농촌진흥청 국립식량과학원) ;
  • 백성범 (농촌진흥청 국립식량과학원) ;
  • 우관식 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 정건호 (농촌진흥청 국립식량과학원) ;
  • 김미정 (농촌진흥청 국립식량과학원) ;
  • 정광호 (농촌진흥청 국립식량과학원) ;
  • 권영업 (농촌진흥청 국립식량과학원)
  • Received : 2012.07.20
  • Accepted : 2012.10.24
  • Published : 2012.12.31
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Abstract

This study was carried out to evaluate antioxidant and anticancer activity of crudes extracts from colored corn (Zea mays. L.) among particle size with different pulverizing methods (pin mill and ultra fine pulverizer). In cytotoxicity test using extracts from the flours grounded by pin mill and ultra fine pulverizer respectively, it showed that A-549 was the highest anticancer activity among in vitro using 4 cancer cell line types (Hep-G2, A-549, HCT-116 and MCF-7). Assessing the antioxidant activities of crude extracted from different pulverizing methods was measured by trolox equivalent antioxidant capacity (TEAC) and ferric reducing antioxidant power (FRAP). Higher scanvening activity against free radical was observed in the crude extracted from small particle size flours of colored corn than in those of the big particle size.

본 연구에서는 검정찰옥수수 종실을 일반분쇄와 저온미세분쇄를 하여 입자크기에 따른 항산화 활성과 cytotoxicity를 평가하여 이용성 증진을 하고자 연구를 수행하였다. 본 연구의 결과를 요약하면 다음과 같다. 1. 분쇄 정도에 따른 평균값은 일반분쇄가 $473.7{\mu}m$, 저온미세분쇄 $17.2{\mu}m$이었으며 중간값은 $336.9{\mu}m$와 $13.4{\mu}m$를 나타내었다. 2. TEAC 활성 측정 결과 일반분쇄는 $3.87{\mu}mol$ TE/g로서 저온미세분쇄 $5.15{\mu}mol$ TE/g보다 낮은 활성을 보였다. FRAP 활성 측정에서는 저온미세분쇄가 $10.08{\mu}mol$ Fe(II)/g로 일반분쇄 $8.86{\mu}mol$ Fe(II)/g보다 높은 활성을 보였다 3. 간암 세포주(Hep-G2) 성장억제에 미치는 영향은 1 mg/ml 농도에서 검정찰옥수수 종실을 일반분쇄(31.48%)한 것보다 저온미세분쇄(27.41%)를 한 경우가 암세포 생장 억제 효과를 큰 것으로 나타났다. 4. 대장암 세포주인 HCT-116에서는 1 mg/ml에서 분쇄정도에 따라 큰 차이를 보이지 않았으며, 유방암 세포주(MCF-7)에서는 일반분쇄가 미세분쇄보다 높은 생장억제를 보였다.

Keywords

References

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