Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Anti-inflammatory Activity of the Undaria pinnatifida Water Extract

미역(Undaria pinnatifida) 물 추출물의 염증 억제 활성

  • Jeong, Da-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences/Pukyong National University) ;
  • Kang, Bo-Kyeong (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Jung, Seul-A (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Hyun-Jee (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Jeong, Hee-Ye (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Bark, Si-Woo (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
  • Received : 2012.10.31
  • Accepted : 2012.11.06
  • Published : 2012.12.31
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Abstract

The anti-inflammatory effects of Undaria pinnatifida water extract (UPWE) were investigated using lipopolysaccharide-induced inflammatory response in this study. To examine the potential anti-inflammatory properties of UPWE, the cell proliferation, nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-${\alpha}$) and IL-$1{\beta}$ were measured. As a result, there was no cytotoxicity in the macrophage proliferation treated with UPWE compared to the control. NO levels decreased with increasing concentration of UPWE. Moreover, the secretion of IL-6, TNF-${\alpha}$ and IL-$1{\beta}$ were suppressed in a dose-dependent manner, and IL-6 inhibition activities were over 50% at 0.1%. These results suggested that UPWE may have significant effects on inflammatory factors and be a potential anti-inflammatory therapeutic materials.

미역 물 추출물의 염증 억제 활성을 알아보기 위하여 nitric oxide(NO) 분비량, tumor necrosis factor-alpha (TNF-${\alpha}$), interleukin-6 (IL-6) 및 IL-$1{\beta}$ cytokine 분비량, RAW 264.7 세포의 증식능을 측정하였다. Lipopolysaccharide (LPS)로 염증이 유도된 대식세포의 NO 분비능에 미치는 미역 물 추출물의 영향을 알아보기 위해 대식세포 배양액의 $NO_2{^-}$ 농도를 측정한 결과 NO 분비량이 미역 추출물 농도에 의존적으로 유의적인 감소 경향을 나타내는 것을 확인하였으며, 모든 첨가 농도에서 TNF-${\alpha}$, IL-6 및 IL-$1{\beta}$ cytokine 분비량이 감소하는 것을 확인하였다. 특히 IL-6 cytokine의 경우 첨가물 농도에 의존적으로 유의적인 감소를 보였다. 미역 물 추출물 첨가에 의한 TNF-${\alpha}$, IL-6 및 IL-$1{\beta}$ cytokine 및 NO 분비량의 감소가 세포 사멸에 의한 영향인지를 알아보기 위해 마우스 복강 대식세포에 대하여 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay를 실시하였다. 그 결과 모든 첨가 농도에서 대식세포 증식능이 control에 비해 유의적으로 증가하였다. 따라서 미역 물추출물은 세포사멸에 의해 cytokine 분비량이 감소한 것이 아님을 확인하였으며, 대식세포 증식능을 증가시켜 면역세포를 활성화 시키는 것으로 사료되어진다.

Keywords

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