Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Antioxidative Effect and Tyrosinase Inhibitory Activity of Lindera obtusiloba Blume Extracts

생강나무 추출물의 항산화 효과 및 타이로시네이즈 저해 활성

  • Won, Doo Hyun (Department of Fine Chemistry, Cosmetic R&D Center, College of Energy and Biotechnology, Seoul National University of Science and Technology) ;
  • Han, Saet Byeol (Department of Fine Chemistry, Cosmetic R&D Center, College of Energy and Biotechnology, Seoul National University of Science and Technology) ;
  • Hwang, Jun Pil (Department of Fine Chemistry, Cosmetic R&D Center, College of Energy and Biotechnology, Seoul National University of Science and Technology) ;
  • Kim, Su Ji (Department of Fine Chemistry, Cosmetic R&D Center, College of Energy and Biotechnology, Seoul National University of Science and Technology) ;
  • Park, Jino (DAEBONG LS) ;
  • Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D Center, College of Energy and Biotechnology, Seoul National University of Science and Technology)
  • 원두현 (서울과학기술대학교 에너지바이오대학 정밀화학과, 화장품종합기술연구소) ;
  • 한샛별 (서울과학기술대학교 에너지바이오대학 정밀화학과, 화장품종합기술연구소) ;
  • 황준필 (서울과학기술대학교 에너지바이오대학 정밀화학과, 화장품종합기술연구소) ;
  • 김수지 (서울과학기술대학교 에너지바이오대학 정밀화학과, 화장품종합기술연구소) ;
  • 박진오 (대봉엘에스(주)) ;
  • 박수남 (서울과학기술대학교 에너지바이오대학 정밀화학과, 화장품종합기술연구소)
  • Received : 2012.06.11
  • Accepted : 2012.12.24
  • Published : 2012.12.30
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Abstract

In this study, the antioxidative effects and inhibitory activities on tyrosinase of Lindera obtusiloba Blume (L. obtusiloba Blume) extracts were investigated. 50 % ethanol extract, ethyl acetate and aglycone fractions of L. obtusiloba Blume were used in experiments. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities of ethyl acetate fraction of L. obtusiloba Blume was higher than (+)-${\alpha}$-tocopherol, known as a typical antioxidant. Reactive oxygen species (ROS) scavenging activities of extract and fractions of L. obtusiloba Blume on ROS generated in $Fe^{3+}$-EDTA/$H_2O_2$ system were similar to L-ascorbic acid, well known as a strong antioxidant. The cellular protective effects of 50 % ethanol extract and ethyl acetate of L. obtusiloba Blume on the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner ($1{\sim}25{\mu}g/mL$). Ethyl acetate fraction in $10{\mu}g/mL$ concentration showed the most protective effect among extracts (${\tau}_{50}$ = 361.0 min). The inhibitory effects on tyrosinase of ethyl acetate and aglycone fractions were higher than arbutin, known as a whitening agent. These results indicate that L. obtusiloba Blume extracts can be used as antioxidant, and could be applicable to functional cosmetic ingredient.

본 연구의 목적은 생강나무 추출물의 항산화 활성과 타이로시네이즈 저해 활성을 살펴봄으로써 화장품 원료로서의 응용 가능성을 확인하는 것이다. 모든 실험은 생강나무의 50 % 에탄올 추출물, 에틸아세테이트(ethyl acetate) 분획, 아글리콘(aglycone) 분획을 이용하여 진행하였다. 에틸아세테이트 분획의 DPPH (1,1-diphenyl-2-picrylhydrazyl) 소거활성은 기존에 잘 알려져 있는 항산화제인 (+)-${\alpha}$-tocopherol 보다 높은 것으로 나타났다. $Fe^{3+}$-EDTA/ $H_2O_2$ 계에서 생성된 활성산소종에 대한 세 분획의 소거활성(총항산화능)은 대표적인 항산화제인 L-ascorbic acid와 비슷한 것으로 나타났다. Rose-bengal로 증감된 $^1O_2$에 의한 적혈구 파괴에서, 50 % 에탄올 추출물과 에틸아세테이트 분획의 세포보호 효과는 농도 의존적($1{\sim}25{\mu}g/mL$)으로 증가하였다. $10{\mu}g/mL$ 농도를 기준으로 비교하였을 때 에틸아세테이트 분획의 ${\tau}_{50}$은 361.0 min으로 가장 높은 세포 보호 활성을 나타내었다. 타이로시네이즈 저해활성에서 에틸아세티이트 분획과 아글리콘 분획은 알부틴보다 높은 저해 효과를 나타내었다. 이상의 결과들로부터 생각나무 추출물은 활성산소종을 소거하는 항산화제로 여러 산업 분야에 응용 가능할 것이라 생각된다. 또한 알부틴을 대체하는 미백 기능성 소재로서 응용될 수 있는 가능성을 확인하였다.

Keywords

References

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