Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Development of Rapid Detection Kit for Aflatoxin B1 in Cereals

곡류 중 아플라톡신 B1 신속 검출용 간이진단키트의 개발

  • Choi, Sunmi (Department of Food and Nutrition, Hanyang University) ;
  • Kim, Giyoung (Department of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Sangdae (Department of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration) ;
  • Moon, Jihea (Department of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration) ;
  • Om, Aeson (Department of Food and Nutrition, Hanyang University)
  • 최선미 (한양대학교 식품영양학과) ;
  • 김기영 (농촌진흥청 국립농업과학원 농업공학부) ;
  • 이상대 (농촌진흥청 국립농업과학원 농업공학부) ;
  • 문지혜 (농촌진흥청 국립농업과학원 농업공학부) ;
  • 엄애선 (한양대학교 식품영양학과)
  • Published : 2013.08.31
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Abstract

The aim of this study is to develop the one dot rapid detection kit that meets the limit of detection (LOD) of KFDA (10 ${\mu}g/kg$) for Aflatoxin $B_1$ (AFB1). The fabrication process of the one dot rapid detection kit, such as gold nanoparticle and AFB1-BSA conjugation, and AFB1-polyclonal antibody concentration was optimized. Fabricated rapid detection kits were tested with the standard AFB1 solutions and the solutions extracted from contaminated maize samples. The LOD in the standard AFB1 solution and extracted AFB1 solution was 5 ${\mu}g/kg$ for both. This result is lower than the LOD of KFDA in food (10 ${\mu}g/kg$) and satisfies the LOD of EU (2-12 ${\mu}g/kg$) and Japan (10 ${\mu}g/kg$) in food.

Keywords

References

  1. Ammida NHS, Micheli L, Palleschi G. 2004. Electrochemical immunosensor for determination of aflatoxin B1 in barley. Anal. Chim. Acta 520: 159-164. https://doi.org/10.1016/j.aca.2004.04.024
  2. Anfossi L, D'rco G, Calderara M, Baggiani C, Giovannoli C, Giraudi G. 2011. Development of a quantitative lateral flow immunoassay for the detection of aflatoxins in maize. Food Addit. Contam. 28: 226-234. https://doi.org/10.1080/19440049.2010.540763
  3. Babacan S, Pivarnik P, Letcher S, Rand A. 2002. Piezoelectric flow injection analysis biosensor for the detection of Salmonella Typhimurium. J. Food Sci. 67: 314-320. https://doi.org/10.1111/j.1365-2621.2002.tb11403.x
  4. Bazin I, Nabais E, Lopez-Ferber M. 2010. Rapid visual tests: Fast and reliable detection of ochratoxin A. Toxins 2: 2230-2241. https://doi.org/10.3390/toxins2092230
  5. Bennett JW. 1987. Mycotoxins, mycotoxicoses, mycotoxicology and mycopathology. Mycopathlogia 100: 3-5. https://doi.org/10.1007/BF00769561
  6. Bhatnagar D, Yu J, Ehrlich KC. 2002. Toxins of filamentous fungi. Chem. Immunol. 81: 167-206. https://doi.org/10.1159/000058867
  7. Blesa J, Sorianoa JM, Moltoa JC, Marinb R, Manesa J. 2003. Determination of aflatoxins in peanuts by matrix solid-phase dispersion and liquid chromatography. J. Chromatogr. A. 1011: 49-54. https://doi.org/10.1016/S0021-9673(03)01102-6
  8. Campbell TC, Hayes JR. 1976. The role of aflatoxin metabolism in its toxic lesion. Toxicol. Appl. Pharmacol. 35: 199-222. https://doi.org/10.1016/0041-008X(76)90282-9
  9. Choi SM, Kim KY, Om AS, Moon JH, Lee SD, Kim HJ. 2012. The selection of basic platform for improving the sensitivity of neutravidin rapid detection kit. CNU J. Agric. Sci. 39: 613-618. https://doi.org/10.7744/cnujas.2012.39.4.613
  10. Hong SY. 2011. Development of a semi-quantitative lateral flow immunoassay for the analysis of human cartilage oligomeric matrix protein. Ajou university.
  11. Hussein HS, Brasel JM. 2001. Toxicity, metabolism and impact of mycotoxins on humans and animals. Toxicology 167: 101-134. https://doi.org/10.1016/S0300-483X(01)00471-1
  12. Jang MR, Lee CH, Cho SH, Park JS, Kwon EY, Lee EJ, Kim SH, Kim DB. 2007. A survey of total aflatoxins in food using high performance liquid chromatography-fluorescence detector (HPLC-FLD) and liquid chromatography tandem mass spectrometry (LC-MS/MS). Korean J. Food Sci. Technol. 39: 488-493.
  13. Kim GY, Yang GM, Park SB, Kim YH, Lee KJ, Son JY, Kim HJ, Lee SR. 2011. Rapid detection kit for Salmonella Typhimurium. J. Biosystems Eng. 36: 140-146. https://doi.org/10.5307/JBE.2011.36.2.140
  14. Moon JH, Kim GY, Lee SD, Park SB, Kim HJ. 2012a. Optimization of lateral flow assay for the dual-detection of mycotoxins. Food Eng. Prog. 16: 214-218.
  15. Moon JH, Kim GY, Lee SD. 2012b. A gold nanoparticle and AFB1-BSA Conjugates based lateral flow assay method for the analysis of AFB1. Materials 5: 634-643. https://doi.org/10.3390/ma5040634
  16. Park JW. 2006. Analysis of peanut and peanut butter retailed in korea for aflatoxin $B_1$. J. Food Sci. Technol. 38: 309-312.
  17. Schuh M. 1997. Clinical and subclinical events related to the presence of mycotoxins in cattle feed. J. Vet. Med. A. 52: 93-97.
  18. Shim WB, Yang ZY, Kim JS, Kim JY, Kang SJ, Woo GJ, Chung YC, Eremin SA, Chung DH. 2007. Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin $B_1$ in grain and feed samples. J. Microbiol. Biotechnol. 17: 1629-1637.
  19. Xiluan S, Xiaolian Z, Jian T, Xiaohong, Jun Z, Chu FS. 2006. Development of an immunochromatographic assay for detection of aflatoxin $B_1$ in foods. Food Control 17: 256-262. https://doi.org/10.1016/j.foodcont.2004.10.007