The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Diversity and Pathogenicity of Fusarium Species Associated with Grain Mold of Sorghum

수수 이삭곰팡이 증상에서 분리한 Fusarium속 균의 다양성 및 병원성

  • 최효원 (농촌진흥청 국립농업과학원) ;
  • 홍성기 (농촌진흥청 국립농업과학원) ;
  • 이영기 (농촌진흥청 국립농업과학원) ;
  • 김완규 (농촌진흥청 국립농업과학원)
  • Received : 2013.08.30
  • Accepted : 2013.09.12
  • Published : 2013.09.30
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Abstract

Sorghum (Sorghum bicolor Moench) was traditionally grown on a small scale, however, at present its cultivation is getting momentum in terms of food and animal feed crop throughtout the Korea. Grain mold symptoms of the plant were frequently observed during disease surveys in Korea from 2007 to 2009. The symptoms were highly variable. Severely infected grain was fully covered with mold and partially infected grain may look normal or discolored. Ninety isolates of Fusarium species were obtained from the diseased plants collected from several locations in the country. Among the collected Fusarium isolates, 41 were identified as Fusarium thapsinum, 23 as F. proliferatum, 12 as F. graminearum, 5 as F. incarnatum, and 3 as F. equiseti based on their morphological and cultural characteristics. Elongation factor 1 alpha gene sequences of the isolates were used for phylogenetic analysis. Analyses of the sequences revealed that the isolates were confirmed to be identical with related species of NCBI GenBank. Pathogenicity tests showed that three dominant species, F. thapsinum, F. proliferatum and F. graminearum were strongly virulent to grains of sorghum. This study is the first report of sorghum grain mold caused by Fusarium species in Korea.

국내의 수수재배는 주로 소면적으로 이루어지며, 대부분 식용이나 사료용으로 사용되고 있다. 2007년부터 2009년까지 전국의 수수 재배 포장을 조사한 결과, 이삭에 곰팡이가 발생하는 증상을 다수 관찰하였다. 병징은 매우 다양한데, 심하게 감염된 이삭의 경우 곰팡이가 발생하였고, 부분적으로 감염된 경우에는 수수 종실이 변색되거나 불임립이 형성되었다. 총 90개의 Fusarium균을 분리하였으며, 형태적 배양적 특성 조사에 의한 동정 결과, F. thapsinum이 41개, F. proliferatum이 23개, F. graminearum이 12개 분리되었으며, F. incarnatum과 F. equiseti가 각각 5개, 3개 분리되었다. Elongation factor 1 alpha 유전자의 염기분석을 이용한 계통분석 결과, 대부분이 NCBI GenBank에 등록된 각 유전자와 일치하는 결과를 나타냈다. 분리된 병원균의 병원성 검정 결과, 우점종인 F. thapsinum, F. proliferatum, F. graminearum종이 병원성이 강한 것으로 조사되었다. 이 연구는 Fusarium균에 의해 발생한 수수 이삭곰팡이증상에 대한 첫 번째 보고이다.

Keywords

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