Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of Genotype Mutation and Coat Color Phenotype on the Offspring from Mating System of MC1R Genotype Patterns in Korean Brindle Cattle

칡소의 MC1R의 유전자형에 따른 교배 조합이 자손의 모색과 유전자형 변이에 미치는 영향

  • Kim, Sang-Hwan (Institute of Genetic Engineering, Hankyong National University) ;
  • Jung, Kyoung-Sub (Insititute of Livestock and Veterinary Research) ;
  • Lee, Ho-Jun (Institute of Genetic Engineering, Hankyong National University) ;
  • Baek, Jun-Seok (Major in the Animal Life a Environment Science the Graduate School Hankyong National University) ;
  • Jung, Duk-Won (Major in Animal Fusion Biotechnology, Graduate School of Bio & Information Technology, Hankyong National University) ;
  • Kim, Dae-Eun (Department of Bio-Medical Science Laboratory, Kyungbok University) ;
  • Yoon, Jong-Taek (Institute of Genetic Engineering, Hankyong National University)
  • 김상환 (한경대학교 유전공학연구소) ;
  • 정경섭 (충청북도 축산위생연구소) ;
  • 이호준 (한경대학교 유전공학연구소) ;
  • 백준석 (한경대학교 대학원 동물생명환경과학과) ;
  • 정덕원 (한경대학교 미래융합기술대학원 동물바이오융합전공) ;
  • 김대은 (경복대학교 임상병리학과) ;
  • 윤종택 (한경대학교 유전공학연구소)
  • Received : 2013.08.23
  • Accepted : 2013.09.12
  • Published : 2013.09.30
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Abstract

Bovine coat color is decided by the melanocortin receptor 1 (MC1R) genotype mutation and melanogenesis. Specially, in the various cattle breeds, dominant black coat color is expressed by dominant genotype of $E^D$, red or brown is expressed in the frame shift mutation of recessive homozygous e by base pair deletion and wild type of $E^+$ is expressed in various coat colors. However, not very well known about the effected of MC1R genotype mutation on the coat color through family lines in KBC. Therefore, this study were to investigate effect of MC1R genotype mutation on the coat color, and to suggest mating breed system in accordance with of MC1R genotype for increased on brindle coat color appearance. Parents (sire 2 heads and dam 3 heads) and offspring (total : 54 heads) from crossbreeding in KBC family line with the MC1R genotype and phenotype records were selected as experimental animals. The relationship between melanocortin 1 receptor (MC1R) genotypes expression verified by PCR-RFLP, and brindle coat color appearance to the family line of the cross mating breed from MC1R genotype pattern was determined. As a result, 4MC1R genetic variations, $E^+/E^+$ (sire 1), $E^+/e$ (sire 2 and dam 3), $E^+/e$ with 4 bands of 174, 207 and 328 bp (dam 1) and $E^+/e$ with 3 bands of 174, 207, 328 and 535 bp (dam 2) from parents (sire and dam) of KBC. However, 3 genetic variations, e/e (24%), $E^+/E^+$ (22%) and $E^+/e$ (56%) were identified in offspring. Also, brindle coat color expressrated was the e/e with the 0%, $E^+/E^+$ with 67% and $E^+/e$ with 77% from MC1R genotype in offspring on the cross mating of KBC. Furthermore, when the sire had $E^+/e$ genotype and the dam had $E^+/E^+$ with the 3 bands or $E^+/e$ genotype, and both had whole body-brindle coat color, 62% of the offspring had whole body-brindle coat color. Therefore, the seresults, the mating system from MC1R genotype patterns of the sires ($E^+/e$) and dams ($E^+/E^+$ with the 3 bands or $E^+/e$) with brindle coat color may have the highest whole body-brindle coat color expression in their offspring.

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References

  1. Adalsteinsson S, Bjarnadottir S, Vage DI and Jonmundsson JV. 1995. Brown coat color in Icelandic cattle produced by the loci Extension and Agouti. J Hered. 86: 395-398. https://doi.org/10.1093/oxfordjournals.jhered.a111609
  2. Chung ER, Kim WT, Kim YS and Han SK. 2000. Identification of Hanwoo meat using PCR-RFLP marker of MC1R gene associated with bovine coat color. J. Anim. Sci. & Technol. 42: 379-390.
  3. Cone RD, Lu D, Koppula S, Vage DI, Klungland K, Boston B, Chen W, Orth DN, Pouton C and Kesterson, RA. 1996. The Melanocortin receptors : agonists, antagonists, and the hormonal control of pigmentation. Recent. Prog. Horm. Res. 51: 287-317.
  4. Do KT, Shin HY, Lee JH, Kim NS, Park EW, Yoon DH and Kim KS. 2007. Investigation of coat color candidate genes in Korean cattle(Hanwoo). J. Anim. Sci. & Technol. 49: 711-718. https://doi.org/10.5187/JAST.2007.49.6.711
  5. Jackson IJ. 1993. Molecular genetics. Colour-coded switches. Nature. 362: 587-588 https://doi.org/10.1038/362587a0
  6. Jackson IJ. 1994. Molecular and developmental genetics of mouse coat color. Annu. Rev. Genet. 28: 189-217. https://doi.org/10.1146/annurev.ge.28.120194.001201
  7. Jin S, Shim JM, Seo DW, Jung WY, Ryoo SH, Kim JH and Lee JH. 2011. Analysis of MC1R genotypes in three different colored Korean cattle (Hanwoo). CNU J. Agri. Sci. 38: 453-458.
  8. Joerg H, Fries HR, Meijerink E and Stranzinger GF. 1996. Red coat color in Holstein cattle is associated with a deletion in the MSHR gene. Mamm Genome. 7: 317-318. https://doi.org/10.1007/s003359900090
  9. Kim TH, Yoon DH, Park EW, Lee HY, Oh SJ, Cheong IC, Thak TY, Kim KN and Han JY. 2000. A study on genotype frequencies of the bovine melanocortin receptor 1 (MC1R) in cattle breeds. J. Anim. Sci. & Technol. 42: 735-744.
  10. Klungland H, Vae DI, Gomez-Raya L, Adalsteinsson S and Lien S. 1995. The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat color determination. Mamm Genome 6: 636-639. https://doi.org/10.1007/BF00352371
  11. Lee HJ, Hwang SS and Yoon JT. 2007. Effects of bovine somatotropin( bst) administrstion combined with controlled internal drug release(CIDR) on embryo quality and pregnancy of Hanwoo(Korean native beef cattle) during commercial transfer program. Asian-Aust. J. Anim. Sci. 20: 194-199.
  12. Lee HJ, Kim SH, Lee KT and Yoon JT. 2011. Characteristics of coat color distribution of offsprings produced by embryo transfer in Korean native brindle cattle. Dev. Re- prod. 15: 325-329.
  13. Lee SS, Yang YH, Kang SY, Oh WY, Yang BS, Ko SB, Oh SJ and Kim KI. 2000. Comparison of the genotypes and frequencies of MSH receptor(MC1R) gene in Korean cattle, Cheju native black cattle, Japanese black and Japanese brown cattle. J. Anim. Sci. & Technol. 42: 253-260.
  14. Lee SS, Yang BS, Yang YH, Kang SY, Ko SB, Jung JK, Oh WY, Oh SJ and Kim KI. 2002. Analysis of melanocortin receptor 1(MC1R) genotype in Korean brindle cattle and Korean cattle with dark muzzle. J. Anim. Sci. & Technol. 44: 23-30. https://doi.org/10.5187/JAST.2002.44.1.023
  15. Park YS, Hwang HS, Yoo JW and Kim NW. 2007. Characteristics of semen and coat color distribution of offsprings produced by al in Korean native striped cattle(Bos namadicus Falconer, Chikso). Reprod. Dev. Biol. 31: 43-48
  16. Park JH, Lee HL, Kim YS and Kim JG. 2012. MC1R Genotypes, coat color, and muzzle phenotype variation in Korean native brindle cattle. J. Anim. Sci. & Technol. 54: 255-265. https://doi.org/10.5187/JAST.2012.54.4.255
  17. Robbins LS, Nadeau JH, Johnson KR, Kelly MA, Roselli-Rehfuss L, Baack E, Mountjoy KG and Cone RD. 1993. Pigmentation phenotypes of variant extension locus alleles results from point mutations that alter MSH receptor function. Cell 72: 827-834. https://doi.org/10.1016/0092-8674(93)90572-8
  18. Royo LJ, Alvarez I, Fernadez I, Arranz JJ, Goez E and Goyache F. 2005. The coding sequence of the ASIP gene is identical in nine wild-type coloured cattle breeds. J. Anim. Breed Genet. 122: 357-60. https://doi.org/10.1111/j.1439-0388.2005.00541.x
  19. Selz Y, Braasch I, Hoffmann C, Schmidt C, Schultheis C, Schartl M and Volff JN. 2007. Evolution of melanocortin receptors in teleost fish : the melanocortin type 1 receptor. Gene 401: 114-122. https://doi.org/10.1016/j.gene.2007.07.005
  20. Sohn SH, Lee CY, Kim DH, Park GB, Lee JG, Shin CK, Chung HS, Kwack SC, Park MK, Chun MS, Baik CS and Ko YD. 2000. Chromosomal pattern and karyotype of the Korean native stripped cattle Chickso. J. Anim. Sci. & Technol. 42: 1-8.
  21. Slominski A, Tobin DJ, Shibahara S and Wortsman J. 2004. Melanin pigmentation in mammalian skin and its hormonal. regulation. Physiol. Rev. 84: 1155-1228. https://doi.org/10.1152/physrev.00044.2003