Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Investigation of Microbial Contamination in Oenanthe javanica at Postharvest Environments

미나리(Oenanthe javanica) 수확 후 처리 환경에서의 위생지표세균 및 병원성 미생물 오염도 조사

  • Kim, Yeon Rok (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Kyoung Ah (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Academy of Agricultural Science, Rural Development Administration) ;
  • Choi, In-Wook (Department of Infection Biology, School of Medicine, Chungnam National University) ;
  • Lee, Young-Ha (Department of Infection Biology, School of Medicine, Chungnam National University) ;
  • Kim, Se-Ri (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Won-Il (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ryu, Song Hee (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Hyo Sub (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ryu, Jae-Gee (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Hwang-Yong (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Academy of Agricultural Science, Rural Development Administration)
  • 김연록 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 이경아 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 최인욱 (충남대학교 의과대학 감염생물학과) ;
  • 이영하 (충남대학교 의과대학 감염생물학과) ;
  • 김세리 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김원일 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 류송희 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 이효섭 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 류재기 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김황용 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀)
  • Received : 2014.09.19
  • Accepted : 2014.11.07
  • Published : 2014.12.31
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Abstract

This study assessed microbiological hazards at postharvest stage of dropwort farms (A, B, C, D, E, F, G, H, I) located in 4 different areas in Korea. The samples were assessed for sanitary indication bacteria (total aerobic bacteria, coliform, and Escherichia coli) and pathogenic bacteria (Escherichia coli O157:H7, Listeria monocytogenes, Staphylococcus aureus and Bacillus cereus). Total aerobic bacteria and coliform in 9 dropwort farms were detected at the levels of 0~7.00 and 0~4.25 log CFU/g, mL, of $100cm^2$. In particular, microbial contamination in worker's hand showed higher than cultivation environment factors. Escherichia coli was detected in several farms of soil, irrigation water, washing water and worker's hand and also, dropwort in these farms was contaminated with E. coli (positive reaction). In case of pathogenic bacteria, B. cereus was detected at the highest levels in soil. S. aureus was detected qualitatively from only one sample of dropwort washed by water. E. coli O157:H7 and L. monocytogenes were not detected. Although dropwort pass through 2 process (trimming and washing), the microbial contamination was not differ significantly before and after which indicates that current washing system was not effect on reduction of microorganism. From these results, the postharvest environment and workers have been considered as cross-contamination factors. Thus, processing equipments and personal hygiene should be managed to reduce the microbial contamination of dropwort. Accordingly management system such as good agricultural practices (GAP) criteria is needed for the safety of dropwort

본 연구는 미나리 수확 후 처리환경의 미생물학적 위해 요소를 조사하기 위해 4개 지역의 미나리 재배농가 9곳을 선정하였다. 미나리 재배농가로부터 다양한 시료를 채취하여 위생지표세균과 병원성 미생물(Escherichia coli O157:H7, Listeria monocytogenes, Staphylococcus aureus and Bacillus cereus)에 대한 오염도를 조사하였다. 먼저, 9개 농가의 재배환경의 미생물 오염도를 분석한 결과 총 호기성 세균과 대장균군이 각각 0~7.00, 0~4.25 log CFU/g, mL, or $100cm^2$ 수준으로 검출되었다. 대장균(E. coli)은 몇몇 농가의 토양, 관개용수, 세척수 및 작업자의 손에서 양성반응을 확인하였으며, 양성반응이 관찰된 농가의 경우 작물인 미나리에서도 양성반응을 보였다. 병원성 미생물 중 B. cereus는 토양에서 가장 많이 검출되었으며, 한 농가의 미나리를 제외한 모든 농가의 세척 후 미나리에서 평균 1.2 log CFU/g수준으로 검출되었다. 황색포도상구균은 세척 후 미나리에서 한 건 정성적으로 검출되었으며, 이외에 다른 병원성 미생물은 관찰되지 않았다. 미나리의 경우 손질 및 세척 후에도 오염도가 유사하거나 오히려 다소 증가하는 경향을 보였다. 이상의 결과 미나리 생산농가의 수확 후 처리 시설 및 작업자에 의해 미생물의 교차 오염 가능성이 있으므로 이에 대한 위생관리를 철저히 해야 한다. 또한, 안전성이 확보된 미나리 생산을 위해서는 GAP와 같은 관리제도의 적용이 필요하다.

Keywords

References

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