KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Study on Monascus Strains and Characteristic for Manufacturing Red Yeast Rice with High Production of Monacolin K

Monacolin K 강화 홍국쌀 생산을 위한 균주 및 특성 연구

  • 박지영 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 한상익 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 서우덕 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 나지은 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 심은영 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 남민희 (농촌진흥청 국립식량과학원 기능성작물부)
  • Received : 2014.03.05
  • Accepted : 2014.04.08
  • Published : 2014.06.30
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Abstract

Red yeast rice (RYR) is the product of fermented yeast by Monascus strains on rice, and has recently become a popular dietary supplement as a traditional food in Asia. RYR contains monacolin K substances known to inhibit cholesterol synthesis as efficiently as statin drugs. To determine the optimal rice cultivar for manufacturing RYR, 7 rice cultivars (Goami, Goami2, Sangjuchalbyeo, Seolgaeng, Saegyejinmi, Yeonghojinmi and Chilbo) were fermented using two Monascus strains (M. ruber KCTC6122 and KCCM60141 of M. ruber) in this study. The monacolin K content of Sangjuchalbyeo were 47.24 ppm on KCTC6122 cultures and 117.03 ppm on KCCM60141 cultures, respectively. Other cultivars, especially Goami and Goami2, which had less content of monacolin K could not seem to ferment normally because those didn't show red color. These results imply that Sangjuchalbyeo can be optimal rice cultivar as a commercial RYR which is well fermented rice and has high content of monacolin K.

혈중 콜레스테롤 저하작용이 있는 monacolin K 고함유 홍국쌀 생산을 위해 2종의 홍국 균주와 7종의 쌀 품종을 사용하여 홍국쌀을 생산하였다. 생산된 홍국쌀의 monacolin K 함량을 분석한 결과는 다음과 같다. 1. 발효된 홍국쌀의 색은 균주에 비해 품종에 따라 차이가 크게 나는 경향이었으며, 상주찰벼가 붉은 색소 함량이 높았지만, monacolin K 생산량과 붉은 색소의 정도가 상관관계가 크다고 판단할 수 없다. 2. 홍국추출물을 UPLC로 이용하여 활성형 monacolin K와 비활성형 monacolin K가 분리 되었고, LC-MS/MS로 분자량을 확인한 결과, 활성형과 비활성형 monacolin K의 선구이온(precursor ion)은 각각 m/z 421, 405였고, 생성이온(product ion) 중 활성형은 m/z 101,319, 비활성형은 m/z 199, 285의 분자량을 확인하였다. 3. M. ruber KCTC6122과 M. ruber KCCM60141 균주를 이용하여 쌀 품종별로 생산한 홍국쌀의 monacolin K 함량을 분석한 결과, 상주찰벼가 각각 47.24, 117.03ppm으로 가장 많은 함량을 나타내었다. 4. 7종의 품종을 비교했을 때, M. ruber KCCM60141이 KCTC6122과 비교하여 monacolin K 생산량이 더 높은 균주이고, 그 중 상주찰벼를 이용하는 것이 홍국쌀 생산에 적절한 품종 선택이라고 판단된다.

Keywords

References

  1. Ana, M., S. Miranda, and N. Biljana. 2013. Development of a rapid LC/DAD/FLD/MSn method for the simultaneous determination of monacolins and citrinin in red fermented rice products, J. Agric. Food Chem. 61 : 1072-1080 https://doi.org/10.1021/jf304881g
  2. Akihisa, T., H. Tokuda, K. Yasukawa, M. Ukiya, A. Kiyota, and N. Sakamoto. 2005. Azaphilones, furanoisophthalides, and amino acids from the extracts of Monascus pilosus-fermented rice (red-mold rice) and their chemopreventive effects. J Agric Food Chem. 53 : 562-565. https://doi.org/10.1021/jf040199p
  3. Alfred, W. Alberts. 1988. Discovery, biochemistry and biology of lovastatin, The American Journal of Cardiology. 62 : 10-15. https://doi.org/10.1016/0002-9149(88)90494-8
  4. Avular, B., Y. H. Wang, T. S. Smillie, and I. A. Khan. 2009. Quantitative determination of lovastatin from dietary supplements containing red yeast rice extracts by using HPLC-UV-MS and UPLC-UV-MS methods, planta medica. 75 : 72.
  5. Cheng, C. and T. M. Pan. 2011. Protective effect of Monascusfermented red mold rice against alcoholic liver disease by attenuating oxidative stress and inflammatory response. J Agric Food Chem. 59 : 9950-9957. https://doi.org/10.1021/jf202577t
  6. Cho, J. H., N. B. Park, Y. C. Song, U. S. Yeo, U. G. Ha, and K. H. Jung. 2012. 'Saegyejinmi': Multiple disease resistance and mid-late maturing tongil type rice cultivar. Kor. J. Breed. Sci. 44(4) : 611-616. https://doi.org/10.9787/KJBS.2012.44.4.611
  7. Choe, J. S., H. H. Ahn, and H. J. Nam. 2002. Comparison of nutritional composition in Korean rices. K Korean Soc Food SciNutr. 31 : 885-892, https://doi.org/10.3746/jkfn.2002.31.5.885
  8. Heber, D., I. Yip, and J. M. Ashley. 1999. Cholesterol-lowering effects of a proprietary Chinese red-yeast-rice dietary supplement. Am J Clin Nutr 69 : 231-236.
  9. Hong, H. C., H. P. Moon, H. C. Choi, Y. G. Hwang, and Y. G. Kim. 2011. A lodging tolerant, opaque rice cultivar 'Seolgaeng'. Kor. J. Breed. Sci. 43(6) : 532-537
  10. Huh, C. K., J. W. Lee, and Y. D. Kim. 2012. Fermentation and quality characteristics of yakju according to different rice varieties. Korean J. Food Preserv. 19(6) : 925-932. https://doi.org/10.11002/kjfp.2012.19.6.925
  11. KFDA, 2010. Health functional food code, Korea food and drug adminstration, Seoul, Korea.
  12. Kim, J. I., J. K. Chang, N. B. Park, U. S. Yeo, B. G. Oh, and J. H. Kang. 2009. A new high qualilty rice variety with high head rice ratio and milling recovery, "Chilbo" Korean J. Breed. Sci. 41(4) : 547-551
  13. Kim, K. S. and Y. J. Park. 2012. Characteristics of growth, monacolin K and pigment production by Monascus strains on plate culture, Food Engineering Progress, 16(4) : 347-354.
  14. Kong, X. D., H. X. Zhang, and X. J. Liu. 1997. Development of economical characters of japonica variety and prospects of high yield breeding. Jiangsu Agric Sci. 3 : 2-16.
  15. Kwon, C. S. 2012. Antioxidant properties of red yeast rice (Monascus purpureus) Extracts, J. Korean Soc. Food Sci. Nutr. 41(4) : 437-442. https://doi.org/10.3746/jkfn.2012.41.4.437
  16. Kyoun, O. Y., S. H. Oh, H. J. Kim, Y. K. Yoon, H. M. Kim, and M. R. Kim. 2006. Analyses of nutrients and antinutrients of rice cultivars. Korean J. Food Cookery Sci. 22 : 949-956.
  17. Lee, C. L., J. J. Wang, S. K. L. Kuo, and T. M. Pan. 2006. Monascus fermentation of dioscorea for increasing the production of cholesterol-lowering agent-monacolin K and anti inflammation agent-monascin. Appl. Microbiol Biotechnol. 72 : 1254-1262. https://doi.org/10.1007/s00253-006-0404-8
  18. Lee, C. L., J. J. Wang, and T. M. Pan. 2008. Red mold rice extract represses amyloid beta peptide-induced neurotoxicity via potent synergism of anti-inflammatory and antioxidative effect. Appl. Microbiol. Cell Physiol. 79 : 829-841.
  19. Li, C. L., Y. Zhu, and Y. Wang. 1998. Monascus purpureusfermented rice (red yeast rice): a natural food product that66 lowers blood cholesterol in animal models of hypercholesterolemia. Nutr. Res. 18 : 71-81. https://doi.org/10.1016/S0271-5317(97)00201-7
  20. Martinkova, L., P. Patakova-Juzlova, V. Krent, Z. Kucerova, V. Havlicek, P. Olsovsky. 1999. Biological activities of oligoketide pigments of Monascus purpureus. Food Addit Contam. 16 : 15-24. https://doi.org/10.1080/026520399284280
  21. Ma, J., Y. Li, Q. Ye, J. Li, Y. Hua, D. Ju, D. Zhang, R. Cooper, and M. Chang. 2000. Constituents of red yeast rice, a traditional chinese food and medicine. J Agric Food Chem. 48 : 5220-5225. https://doi.org/10.1021/jf000338c
  22. Patakova, P. 2005. Red yeast rice. In: McGraw-Hill yearbook of science and technology, McGraw-Hill, New York. 286-288. ISBN 0-07-144504-8.
  23. Seo, J. W. and Y. J. Park. 2012. Characteristics of Growth, Pigment and Monacolin K Production by Monascus strains in Liquid Culture, Korean Society Biotechnology and Bioengineering Journal. 27 : 301-307. https://doi.org/10.7841/ksbbj.2012.27.5.301
  24. Song, Y. C., S. J. Lim, J. S. Lee, and H. Y. Kim. 2008. A New high amylose rice variety "Goamibyeo," Korean J. Breed. Sci. 40(4) : 447-451.
  25. Stork, C. R., L. P. Silva, and C. A. A. Fagundes. 2005. Catergorizing rice cultivars based on differences in chemical composition. J Food com Anal, 18 : 333-341. https://doi.org/10.1016/j.jfca.2004.09.005
  26. Ryu, K. R., Y. C. Song, S. J. Lim, and S. C. Kim. 1988. A waxy rice variety with semi-early maturity, high quality, high yielding and resistance to blast "Sangjuchalbyeo." Korean J. Breed. Sci. 30(4) : 386.
  27. Taira, J., C. Miyagi, and Y. Aniya. 2002. Dimerumic acid as an antioxidant from the mold, Monascus anka: the inhibition mechanisms against lipid peroxidation and hemeproteinmediated oxidation. Biochem. Pharmacol. 63 : 1019-1026. https://doi.org/10.1016/S0006-2952(01)00923-6
  28. Wang, J., Z. Lu, and J. Chi. 1997. A multi-center clinical trial of the serum lipid lowering effects of a Monascus purpureus (red yeast) rice preparation from traditional Chinese medicine. Curr Ther Res. 58 : 964-978. https://doi.org/10.1016/S0011-393X(97)80063-X
  29. Yeo, U. S., C. S. Kim, J. H. Lee, D. Y. Kwak, J. H. Cho, D. S. Park, and Y. C. Song. 2012. 'Yeonghojinmi': High grain quality, multiple disease resistance, and mid-late rice cultivar, Kor. J. Breed. Sci. 44(2) : 180-184.

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