Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Illustration of Disease Suppression of Anthracnose on Cucumber Leaves by Treatment with Chlorella fusca

오이 잎에서 Chlorella fusca 처리에 의한 오이탄저병 발생 억제 기작

  • Lee, Yun Ju (Major of Plant Resources and Environment, Faculty of Bioscience and Industry, College of Applied Life Sciences, Jeju National University) ;
  • Ko, Yun Jung (Major of Plant Resources and Environment, Faculty of Bioscience and Industry, College of Applied Life Sciences, Jeju National University) ;
  • Jeun, Yong Chull (Major of Plant Resources and Environment, Faculty of Bioscience and Industry, College of Applied Life Sciences, Jeju National University)
  • 이윤주 (제주대학교 생명자원과학대학 생물산업학부 식물자원환경전공) ;
  • 고윤정 (제주대학교 생명자원과학대학 생물산업학부 식물자원환경전공) ;
  • 전용철 (제주대학교 생명자원과학대학 생물산업학부 식물자원환경전공)
  • Received : 2016.08.17
  • Accepted : 2016.11.08
  • Published : 2016.12.31
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Abstract

Chlorella is known as chlorophyceae which can live autotrophically by photosynthesis, promote the growth of plants and suppress some plant diseases. However, a few researches in inhibition mechanism of plant diseases by chlorella have been carried out. In this study cucumber leaves pre-treated with Chlorella fusca suspension were investigated whether anthracnose by Colletotrichum orbiculare is suppressed or not. Furthermore, in order to illustrate how the algae can restrain the antracnose, the infection structures of C. orbiculare were observed on the cucumber leaves pre-treated with the algae. Consequently, appressorium formation rate was apparently reduced in the cucumber leaves pre-treated with C. fusca compared to untreated control one. Also, the numbers of conidia found at the inoculation sites were significantly reduced compared to untreated one. On the other hand, on the leaves pre-treated with $Benomyl^{(R)}$ appressorium formation were decreased remarkably and numbers of conidia were also reduced similar with those pre-treated with C. fusca. Based on these results, it was revealed that occurrence of anthracnose can be suppressed by C. fusca pre-treatment and suggested that biochmical or structural hinderance by C. fusca resulting in the decline of appressorium formation on the leave surfaces may play an important role in the disease suppression.

클로렐라는 광합성을 통해 독립적으로 생활할 수 있는 녹조류로서 작물의 생육촉진효과와 더불어 식물병 발생을 억제한다고 알려져 있다. 그러나 클로렐라에 의한 식물병 억제 기작에 대해서는 연구가 미흡한 실정이다. 본 연구에서 클로렐라 일종인 C. fusca의 현탁액을 오이 잎에 전처리하였을 때 오이탄저병 진전이 억제되는지 여부를 조사하였으며, C. fusca를 전처리한 오이 잎에서 오이탄저병 억제 기작을 알아보았다. 그 결과, 무처리한 잎에 비해 C. fusca를 전처리한 잎에서 C. orbiculare의 부착기 형성률이 뚜렷하게 감소하였다. 또한 접종 부위에서 발견된 포자수도 무처리한 잎에 비해 유의성 있게 감소하였다. 한편, 살균제 $Benomyl^{(R)}$을 전처리한 오이 잎에서도 C. orbiculare의 부착기 형성률이 크게 감소하였으며 포자수도 C. fusca를 전처리한 잎과 유사하게 감소되었다. 따라서 이들 결과를 통해 C. fusca 전처리에 의해 오이탄저병 발생이 억제되며 이는 C. fusca에 의한 식물잎 표면에서 생화학적 또는 구조적 작용으로 인한 C. orbiculare의 부착기 형성 감소가 그 원인 중 하나일 것이라고 생각된다.

Keywords

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