Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Antioxidant and Cellular Protective Effects of Parthenocissus tricuspidata Stem Extracts Fermented by Lactobacillus pentosus

Lactobacillus pentosus 발효에 의한 담쟁이덩굴 줄기 추출물의 항산화 및 세포보호 효과

  • Park, So Hyun (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology) ;
  • Seong, Joon Seob (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology) ;
  • Lee, Keon Soo (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology) ;
  • Park, Young Min (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology) ;
  • Xuan, Song Hua (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology) ;
  • Cha, Mi Yeon (GFC Co., Ltd.) ;
  • Kang, Hee Cheol (GFC Co., Ltd.) ;
  • Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology)
  • 박소현 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 성준섭 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 이건수 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 박영민 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 현송화 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터) ;
  • 차미연 ((주)지에프씨 생명과학연구원) ;
  • 강희철 ((주)지에프씨 생명과학연구원) ;
  • 박수남 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융.복합산업 지원 센터)
  • Received : 2017.06.04
  • Accepted : 2017.09.22
  • Published : 2017.09.30
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Abstract

In this study, the antioxidant activities, cellular protective effects, and inhibitory effects on elastase of non-fermented and fermented extracts of Parthenocissus tricuspidata (P. tricuspidata) stem using Lactobacillus pentosus were investigated. The free radical scavenging activities ($FSC_{50}$) of non-fermented and fermented extracts were 42.3 and $34.5{\mu}g/mL$, respectively, in which the activity after fermentation was approximately 18.4% higher. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) in $Fe^{3+}-EDTA/H_2O_2$ system of non-fermented and fermented extracts were 2.6 and $2.5{\mu}g/mL$, respectively. The activity after fermentation was approximately 4.2% higher. In the $^1O_2$-induced cellular damage of erythrocytes, the cellular protective effects (${\tau}_{50}$) of non-fermented and fermented extracts were 126.4 and 173.0 min at $50{\mu}g/mL$, respectively. The activity after fermentation was approximately 34.0% higher. The effect of fermented extract was 3.9 times higher than $(+)-{\alpha}$-tocopherol (${\tau}_{50}=43.4min$), known as a lipophilic antioxidant at $50{\mu}g/mL$. The inhibitory effect of elastase was investigated to predict the anti-wrinkle efficacy using Hs68 human fibroblasts cells. The elastase inhibitory activities ($IC_{50}$) of non-fermented and fermented extracts were 873.6 and $687.8{\mu}g/mL$, respectively, and the activity after fermentation was approximately 21.3% higher. These results indicated that fermented extract of P. tricuspidata stem has potentials as natural cosmetic ingredients with antioxidant and anti-wrinkle effect.

본 연구에서는 담쟁이덩굴 줄기 70% 에탄올 추출물과 발효균주 Lactobacillus pentosus를 이용하여 발효시킨 담쟁이덩굴 줄기 발효추출물에 대하여 항산화 및 세포보호 효과를 측정하였다. 1,1-Diphenyl-2-picrylhydrazyl(DPPH)를 이용한 자유라디칼 소거 활성($FSC_{50}$)은 담쟁이덩굴 줄기 추출물 및 발효추출물이 각각 42.3 및 $34.5{\mu}g/mL$로 발효 후의 라디칼 소거활성이 약 18.4% 더 높게 나타났다. Lumiol-의존성 화학발광법을 이용한 $Fe^{3+}-EDTA/H_2O_2$계에서의 총 항산화능($OSC_{50}$) 평가에서도 담쟁이덩굴 줄기 추출물과 발효추출물은 각각 2.6 및 $2.5{\mu}g/mL$로 발효 후가 약 4.2% 정도 더 높은 총 항산화능을 나타냈다. $^1O_2$로 유도된 적혈구 세포 손상에 있어서 추출물 및 발효추출물의 세포 보호 효과(${\tau}_{50}$)는 $50{\mu}g/mL$에서 각각 126.4 및 173.0 min을 나타내어 발효 후 세포 보호 효과가 약 34.0% 더 높게 나타났다. 발효추출물은 지용성 항산화제로 알려진 $(+)-{\alpha}$-tocopherol (43.4 min)보다도 3.9배 높은 세포 보호 활성을 보여주었다. 사람 섬유아세포인 Hs68을 대상으로 elastase 저해 활성을 조사하였다. Elastase 저해 활성($IC_{50}$)은 담쟁이덩굴 줄기 추출물과 발효추출물에서 각각 873.6 및 $687.8{\mu}g/mL$로 발효 후에 elastase 저해 활성이 약 21.3% 더 높은 것으로 나타났다. 이상의 결과들은 담쟁이덩굴 줄기 발효추출물이 항산화 작용과 더불어 주름개선 효과를 가지는 천연 화장품 소재로써 응용 가능성이 있음을 시사한다.

Keywords

References

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