Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지

The Anticancer Effect of Extracts from Vitex rotundifolia on Human Colon Carcinoma Cell Lines

대장암 세포주에 대한 만형자(Vitex rotundifolia) 추출물의 항암 효과

  • Jo, Kyung-Jin (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Yoon, Mi-Young (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Mi-Ra (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Cha, Mi-Ran (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
  • 조경진 (경남대학교 식품생명학과) ;
  • 윤미영 (경남대학교 식품생명학과) ;
  • 이미라 (경남대학교 식품생명학과) ;
  • 차미란 (경남대학교 식품생명학과) ;
  • 박해룡 (경남대학교 식품생명학과)
  • Published : 2007.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the cytotoxic activity from Vitex rotundifolia. V. rotundifolia was extracted with methanol, ethanol, and acetone, and then the cytotoxic effect of these extracts was measured by the MTT reduction assay and morphological assay on the HT-29 human colon carcinoma cells. Among the three extracts, the acetone extract showed the highest cytotoxic activity on the HT-29 cells in a dose-dependent manner with an $IC_{50}$ value of 10 ${\mu}g/ml$. The acetone extract was further fractionated sequentially with n-hexane, diethyl ether, ethyl acetate, and water layer according to the degree of polarity. The n-hexane layer among the fractioned layers showed inhibitory activity on the growth of HT-29 cells. In addition, n-hexane layer also showed the cytotoxic activity against SW620 human colon carcinoma cells. These result indicated that extracts of V. rotundifolia may contain bioactive materials and could be potential candidates as chemotherapeutic agents against human colon carcinoma cells.

본 연구는 만형자(Vitex rotundifolia)의 세포독성을 확인하기 위한 목적으로 methanol, ethanol, acetone으로 추출한 뒤, 인간 유래의 대장암 세포주인 HT-29에 처리하여 MTT reduction assay를 이용하여 실험을 하였다. 그 결과, 모든 만형자 추출물에서 농도 의존적으로 성장 저해 효과가 나타났으며, 그 중에서도 acetone 추출물을 50 ${\mu}g/ml$ 농도로 처리 시 18%로 가장 높은 항암 효과를 보였다. 또한 각 용매별 추출물을 50 ${\mu}g/ml$를 처리하여 HT-29 세포주의 성장을 관찰한 결과, acetone 추출물은 대조구에 비하여 암세포의 수축 등 형태변화가 두드러지게 나타났으며, Hoechst 33342 staining을 통하여 apoptotic body가 형성된 것을 확인 하였다. 그리고 가장 활성이 좋은 acetone 추출물을 용매 분획하여 얻은 분획물 중, n-hexane 분획물에서 농도 의존적으로 세포독성을 나타냄을 알 수 있었다. 또한 만형자의 n-hexane 분획물은 다른 대장암 세포주 SW620에 대해서도 50 ${\mu}g/ml$ 농도에서 대조군에 비하여 7배 정도의 높은 세포독성을 나타내고 있음을 MTT reduction assay와 LDH release assay를 통하여 확인 할 수 있었다.

Keywords

References

  1. Rho, S. N. and Han, J. H. (2000) Cytotoxicity of garlic and onion methanol extract on human lung cancer cell lines. J. Korean Soc. Food Sci. Nutr. 29, 870-874
  2. Doll, R. and Peto, R. (1981) The causes of cancer. J. Natl. Cancer Inst. 66, 1191-1308
  3. Cha, J. Y., Jeon, B. S., Moon, J. C., Yoo, J. H. and Cho, Y. S. (2004) Cytotoxic effect of Inonotus obliquus composition in HCT-15 human colon cancer cells and AGS gastric cancer cells. J. Korean Soc. Food Sci. Nutr. 33, 633-640 https://doi.org/10.3746/jkfn.2004.33.4.633
  4. Shim, S. M., Kim, M. and Bae, S. J. (2001) Cytotoxicity and quinone reductase induced effects of Daucus carota L. leaf extracts on human cancer cells. J. Korean Soc. Food Sci. Nutr. 30, 86-91
  5. Ham, S. S., Cui, C. B., Choi, H. T. and Lee, D. S. (2004) Antimutagenic and cytotoxic effects of Allium victorialis extracts. Korean J. Food Preserv. 11, 221-226
  6. Chung, Y. J., Bae, M. W., Chung, M. I., Lee, J. S. and Chung, K. S. (2002) Cytotoxic effect of the distilled pine-needle extracts on several cancer cell lines in vitro. J. Korean Soc. Food Sci. Nutr. 31, 691-695 https://doi.org/10.3746/jkfn.2002.31.4.691
  7. Cha, M. R., Yoon, M. Y., Kim, J. Y., Hwang, J. H. and Park, H. R. (2006) The cytotoxic effect of the gleditsiae semen extracts on human colon carcinoma cells. J. Korean Soc. Appl. Biol. Chem. 49, 248-253
  8. Kim, K. H., Park, H. Y., Nam, J. H., Park, J. E., Kim, J. Y., Park, M. I., Chung, K. O., Park, K. Y. and Koo, J. Y. (2005) The inhibitory effect of curcumin on the growth of human colon cancer cells (HT-29, WiDr) in vitro. Korea J. Gastroenterol. 45, 277-284
  9. Han, D. S., Oh, S. K. and Oh, E. S. (2003) Selective cytotoxicities of phenolic acids in cancer cells. J. Toxicol. Pub. Health. 19, 45-50
  10. Kim, H. J., Lee, I. S. and Lee, K. R. (1999) Antimutagenic and anticancer effects of Ramaria botrytis (Fr.) Rick extracts. J. Kor. Soc. Food Sci. Nutr. 28, 1321-1325
  11. Ham, S. S., Kim, E. J., Jung, S. W., Choi, K. P., Zhao, J. L. and Hwang, B. H. (1996) The antimutagenic and anticancer effect of Taxus cuspidata extracts. J. Kor. Soc. Food Sci. Nutr. 25, 1062-1068
  12. Goodman, G. E., Yen, P. Y., Cox, C. T. and Crowlny, J. (1987) Effect of verapamilon in vitro cytotoxicin and vinblastine in human tumor cell. Cancer Res. 47, 2295-2304
  13. Einhorn, L. H. and Willinams, S. D. (1980) Chemotherapy of disseminated testicular cancer. Cancer 46, 1339-1344 https://doi.org/10.1002/1097-0142(19800915)46:6<1339::AID-CNCR2820460607>3.0.CO;2-J
  14. Kim, W. S., Lee, R. A., Hwang, D. Y., Hong, Y. J. and Hong, S. I. (2004) Histoculture drug response assay in colorectal cancer specimen. J. Korean Surgical Society 66, 109-115
  15. Tucker, J. M., Davis, C., Kitchens, M. E., Bunni, M. A., Priest, D. G., Spencer, H. T. and Berger, F. G. (2002) Response to 5- fluorouracil chemotheraphy is modified by dietary folic acid deficiency in $Apc^{Min/+}$ mice. Cancer Lett. 187, 153-162 https://doi.org/10.1016/S0304-3835(02)00402-0
  16. Ramming, K. P. and Haskell, C. M. (1985) In Haskell CM (ed) Cancer treatment, (2nd ed.): Colorectal malignancies, Philadephia
  17. Choi, H. R., Lee, K. H. and Kim, C. H. (2003) Radiosensitizing and antitumor effect of the seed of Benincasae hispida. Korean J. Food Sci. Technol. 35, 479-482
  18. Kim, H. S., Hong, S. B., Sung, H. J., Moon, G. A. and Yoon, Y. (2003) Effect of deer blood on reduction of the side effects of chemotherapeutic drugs. Kor. J. Pharmacogn. 34, 145-149
  19. Grigsby, P. W. (2002) Update on radiation therapy for endometrial cancer. Oncology 16, 777-786
  20. Park, R. K., Oh, K. R., Lee, K. G., Mun, Y. J., Kim, J. H. and Woo, W. H. (2001) The water extract of Boswellia carterii induces apoptosis in human leukemia HL-60 cells. Yakhak Hoeji 45, 161-168
  21. Oh, H. S., Cui, C. B., Choi, H. T., Kim, S. H., Jeon, M. S. and Ham, S. S. (2004) Antimutagenic and cytotoxic effects of Acer ginnala Max. bark extracts. Korean J. Food Preser. 11, 550- 556
  22. Kim, Y. M., Do, J. R., Kim, D. S. and Park, J. H. (2006) Cytotoxicities of hydrolyzed crude laminaran from Eisenia bicyclis on the SNU-1, HeLa and SW cells. Korean J. Food Sci. Technol. 38, 793-798
  23. Jung, S. E. and Bae, J. H. (2002) The effect of Prunus mume extracts on the growth of Hepg 2 and HeLa cell lines. Korean J. Food Nutr. 35, 439-445
  24. Park, S. Y., Shin, M. O., Lee, S. H. and Bae, S. J. (2005) The growth inhibitory effects of Atrina Pecitinata fractions on cancer cell lines. Korean Nutr. Soc. 38, 307-312
  25. Im, H. G., Yu, M. H., Chung, D. W. and Lee, I. S. (2006) Inhibitory effects of fungal metabolites isolated from foodstuffs on the growth of human cancer cell lines. Korean J. Food Sci. Technol. 38, 262-267
  26. Park, Y. J., Kim, M. H. and Bae, S. J. (2002) Enhancement of anticarcinogenic effect by combination of lucii fructus with vitamin C. J. Korean Soc. Food Sci. Nutr. 31, 143-148 https://doi.org/10.3746/jkfn.2002.31.1.143
  27. Ko, S. T., Moon, Y. H. and Ko, O. H. (1976) Influence of Viticis rotundifoliae Fructus on the blood pressure of the rabbit. Kor. J. Pharmacogn. 7, 263
  28. Kang, S. S., Kim, J. S., Kim, H. J., Jung, Y. R. and Shin, S. W. (1994) Phytochemical analysis of Viticis Fructus. Kor. J. Pharmacogn. 25, 214-220