KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antibacterial Activity against Food-poisoning Causing Bacteria and Characterization of Lactobacillus plantarum YK-9 Isolated from Kimchi

김치에서 분리한 세균인 Lactobacillus plantarum YK-9의 식중독 원인세균에 대한 항균활성 및 특성

  • Song, You-Jin (Department of Biotechnology, Soonchunhyang University) ;
  • Park, Su-Ho (Department of Biotechnology, Soonchunhyang University) ;
  • You, Ji-Young (Department of Biotechnology, Soonchunhyang University) ;
  • Cho, Yun-Seok (Department of Biotechnology, Soonchunhyang University) ;
  • Oh, Kye-Heon (Department of Biotechnology, Soonchunhyang University)
  • 송유진 (순천향대학교 생명공학과) ;
  • 박수호 (순천향대학교 생명공학과) ;
  • 유지영 (순천향대학교 생명공학과) ;
  • 조윤석 (순천향대학교 생명공학과) ;
  • 오계헌 (순천향대학교 생명공학과)
  • Published : 2009.06.29
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Abstract

The purpose of this work was to investigate the antibacterial activity of Lactobacillus plantarum YK-9 isolated from fermented Kimchi. Morphological and biochemical characteristics of L. plantarum YK-9 were examined. Phylogenetic analysis using 16S rRNA sequencing was performed to identify the strain, and the strain could be assigned to Lactobacillus plantarum, designated as L. plantarum YK-9. The strain was registered in GenBank as [FJ669130]. During the incubation period of L. plantarum YK-9, the changes of bacterial growth and residual organic acids were monitored. HPLC was used to confirm the organic acids produced in the cultures as metabolites. L. plantarum YK-9 produced both lactic acid and acetic acid, which were responsible for the pH decrease during growth. Initial pH 7.0 of the cultures decreased to 3.6 at the incubation after 72 hours, and concentrations of lactic acid and acetic acid increased to approximately 588.7 mM and 255.5 mM, respectively. The antibacterial activities against food-poisoning causing bacteria were examined with 20-fold concentrated culture supernatants from L. plantarum YK-9, and the antibacterial effects were clearly observed against all the bacteria tested in this work.

본 연구는 김치에서 분리한 Lactobacillus plantarum YK-9의 다양한 특성을 조사하기 위하여 실시되었다. 형태학적 관찰 및 생리화학적 특성에 대하여 조사하였다. 16S rRNA 염기서열 분석을 통해 균주를 동정하였고, 그 결과 Lactobacillus plantarum로 확인되었고, L. plantarum YK-9으로 명명하였으며, 이 균주는 GenBank에 [FJ669130]로 등재하였다. 배양 시간에 따른 L. plantarum YK-9의 생장과 pH의 변화를 조사하였으며, 대사산물로서 유기산 (lactic acid와 acetic acid)의 생성은 HPLC를 통하여 확인하였다. 유기산의 생성은 L. plantarum YK-9의 생장에 따라 증가 하는 것을 확인하였다. 초기 pH 7.0은 배양 기간동안 3.6으로 감소하였고, 배양 72시간이 지난 후 생성된 lactic acid와 acetic acid의 농도는 각각 588.7 mM과 255.5 mM이었다. 식중독 원인균인 S. aureus, B. cereus, L. monocytogenes, E. coli, E. faecalis, S. enteritidis에 대하여 20배로 농축된 YK-9 배양 상등액에 처리하여 항균력을 조사하였으며, 실험에 사용된 6가지 식중독 원인세균 모두에 대한 항균효과가 있는 것이 관찰되었고, 이는 유기산 생성에 기인하는 것으로 나타났다.

Keywords

References

  1. Oldfield, E. C. (2001), Emerging foodbome pathogens: keeping your patients and your families safe. Rev. Gstroenterol. Disorders. 1, 177-186
  2. Jung, H. J., and S. H. Park, H. A. Seo, Y. J. Kim, J. I. Cho, S. S. Park, D. S. Song, and K. S. Kim (2005), Differentiation of four major gram-negative foodborne pathogenic bacterial genera by using ERIC-PCR genomic fingerprinting. Kor. J. Food Sci. Technol. 37, 1005-1011
  3. Ahn, S. C., T. K. Kim, H. J. Lee, Y. J. Oh, J. S. Lee, D. O. Kang, W. K. Oh, T. I. Mheen, and J. S. Ahn (2001), Fermentation patterns of leek kimchi and chinese cabbage kimchi. Kor. J. Microbiol. 37, 234-238
  4. Lee, C. W., C. Y. Ko, and D. M. Ha (1992), Microfloral changes of the lactic acid bacteria during Kimchi fermentation and identification of the isolates. Kor. J. Appl. Microbiol. Biotechnol. 20, 102-109
  5. Aly, S., A. T. Cheik, H. N. Imael, and S. Alfred (2004), Antibacterial activities of lactic acid bacteria strains isolated from burkina faso fermentated milk. J. Nutri. 3, 174-179
  6. Baek, Y. J. and H. S. Bae (1988), Growth inhibition of enteropathogenic Escherichia coli $A_2$ and Escherichia coli $G_7$ by the organic acid producing bacteria. Kor. J. Appl. Microbiol. Bioeng. 16, 111-118
  7. Cho, J. S., S. J. Jung, Y. M. Kim, and U. H. Chun (1994), Detection of the bacteriocin from lactic acid bacteria involved in Kimchi fermentation. Kor. J. Appl. Microbiol. Biotechnol. 22, 700-706
  8. Johansson, M. L., G. Molin, B. Jeppsson, S. Nobaek, S. Ahrne, and S. Bengmark (1993), Administration of different Lactobacillus strains in fermented oatmeal soup: in vivo colonization of human intestinal mucosa and effect on the indigenous flora. Appl. Environ. Microbiol. 59, 15-20
  9. Bae, S. S. and C. Ahn (1997), Antibiosis and bacteriocin production of lactic acid bacteria isolated from kimchi. J. Food Sci. Nutr. 2, 109-120
  10. Vrese, M., P. Winkler, P. Rautenberg, T. Harder, C. Noah, C. Laue, S. Ott, J. Hampe, S. Schreiber, K. Heller, J. Schrezenmeir (2006), Probiotics bacteria reduced duration and severity but not the incidence of common cold episodes in a double blind, randomized, controlled trial. Vaccine 24, 6670-6674 https://doi.org/10.1016/j.vaccine.2006.05.048
  11. Chon, H. S., B. R. Choi, G. J. Jeong, and I. P. Mo (2008), Evaluation system for an experimental study of low pathogenic avian influenza virus (H9N2) infection in specific pathogen free chickens using lactic acid bacteria, Lactobacillus plantarum KFCCI1389P. Avian Pathol. 37, 593-597 https://doi.org/10.1080/03079450802439056
  12. Jang, S. Y. and Y. J. Jeong (2005), Effect of chitosanliquid calcium addition on the quality of Kimchi during fermentation. J. Kor. Soc. Food Sci. Nutr. 34, 715-720 https://doi.org/10.3746/jkfn.2005.34.5.715
  13. Ahn, D. K., T. W. Han, H. Y. Shin, I. N. Jin, and S. Y. Ghim (2003), Diversity and antibacterial activity of lactic acid bacteria isolated from kimchi. Kor. J. Microbiol. Biotechnol. 31, 191-196
  14. Krueger, W. B. and B. J. Kolodziej (1986), Laboratory Procedures for General Microbiology, p59, Kendall / Hunt Publishing Company
  15. Cho, Y. S., N. S. Schiller, and K. H. Oh (2007), Cellular responses and proteomic analysis of Escherichia coli exposed to green tea polyphenols. Curr. Microbiol. 53, 501-506 https://doi.org/10.1007/s00284-007-9021-8
  16. Chun, J. W., C. W. Ma, and K. H. Oh (2005), Physiological characterization of Lactobacillus sp. JK-8 isolated from shrimp aquaculture pond. Kor. J. Microbiol. 41, 18-23
  17. Schillinger, U. and F. K. Lucke (1989), Antibacterial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55, 1901-1906
  18. Sameshima, T., C. Magome, K. Takeshita, K. Arihara, M. Itoh, and Y. Kondo (1998), Effect of intestinal Lactobacillus starter cultures on the behaviour of Staphylococcus aureus in fermented sausage. J. Food Microbiol. 41, 1-7 https://doi.org/10.1016/S0168-1605(98)00038-5
  19. Shen, Y., Y. Liu, Y. Zhang, J. Cripe, W. Conway, J. Meng, G. Hall, and A. A. Bhanwat (2006), Isolation and characterization of Listeria monocytogenes isolates from ready-to-eat foods in Florida. Appl. Environ. Microbiol. 72, 5073-5076 https://doi.org/10.1128/AEM.00435-06
  20. Teuber, M. (1999), Spread of antibiotic resistance with food-bome pathogens. Cell. Molecul. Life Sci. 56, 755-763 https://doi.org/10.1007/s000180050022
  21. Andersson, A., U. Ronner, P. E. Granum (1995), What problems does the food industry have with the sporeforming pathogens Bacillus cereus and Clostridium perfringens? Int. J. Food. Microbiol. 28, 145-155 https://doi.org/10.1016/0168-1605(95)00053-4
  22. Wilson, A. R., D. Sigee, and H. A. S. Epton (2005), Anti-bacterial activity of Lactobacillus plantarum strain SKl against Listeria monocytogenes is due to lactic acid production. J. Appl. Microbiol. 99, 1516-1522 https://doi.org/10.1111/j.1365-2672.2005.02725.x
  23. Ma, C. W., Y. S. Cho, and K. H. Oh (2009), Removal of pathogenic bacteria and nitrogens by Lactobacillus spp. JK-8 and JK-11. Aquaculture 287, 266-270 https://doi.org/10.1016/j.aquaculture.2008.10.061
  24. Herich, R. and M. Levkut (2002), Lactic acid bacteria, probiotics and immune system. Vet. Med. 47, 169-180