Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Biological Activities of Hominis Placenta Herbal Acupuncture prepared by Hydrochloric Acid Hydrolysis

염산 가수분해 추출법으로 조제된 자하거 약침액의 생리활성 효과

  • Seo, Geun-Young (Dept. of Meridian & Acupoint, college of Oriental Medicine, Wonkwang University) ;
  • Lee, Sung-Won (Dept. of Meridian & Acupoint, college of Oriental Medicine, Wonkwang University) ;
  • Park, Sung -Joo (Dept. of Herbology, school of Oriental Medicine, Wonkwang University) ;
  • Kim, Sung-Chul (Dept. of Acupuncture & Moxibustion, Gwang-Ju Oriental Medical Hospital, Wonkwang University) ;
  • Sohn, In-Chul (Dept. of Meridian & Acupoint, college of Oriental Medicine, Wonkwang University) ;
  • Hwang, Sung-Yeoun (Korea Bio Medical Science institute) ;
  • Ahn, Seong-Hun (Dept. of Meridian & Acupoint, college of Oriental Medicine, Wonkwang University)
  • 서근영 (원광대학교 한의과대학 경혈학교실) ;
  • 이성원 (원광대학교 한의과대학 경혈학교실) ;
  • 박성주 (원광대학교 한의과대학 본초학교실) ;
  • 김성철 (원광대학교 광주한방병원 침구과) ;
  • 손인철 (원광대학교 한의과대학 경혈학교실) ;
  • 황성연 (한국전통의학연구소) ;
  • 안성훈 (원광대학교 한의과대학 경혈학교실)
  • Received : 2010.04.19
  • Accepted : 2010.06.08
  • Published : 2010.06.30
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Abstract

Reactive Oxygen Species(ROS) are continuously produced at a high rate as a by-product of aerobic metabolism. Since tissue damage by free radical increases with age, the reactive oxygen species(ROS) such as hydrogen peroxide($H_2O_2$), nitric oxide(NO). Several lines of evidence provided that ROS appears to cause to develop aging-related various diseases such as cancer, arthritis, cardiovascular disease. In this study, we have conducted to investigate the biological activities of Hominis Placenta Herbal Acupuncture by measuring total polyphenol content, DPPH radical scavenging, ABTS radical scavenging, Superoxide dismutase(SOD)-like activity, Nitrite scavenging ability in vitro. The total polyphenol contents of Hominis Placenta Herbal Acupuncture was $24.6m{\ell}/m{\ell}$. Elctron donation ability on DPPH was 49.4%. The 2,2'-azinobis-3-ehtlbezothiazoline-6-sulfonic acid radical decolorization (ABTS) was 50.01%, similar 10 the DPPH free radical scavenging. The superoxide dismutase (SOD)-like activities of hominis placenta herbal acupuncture was 50.876%. The nitrite scavenging abilities at pH 1.5, pH 3.0, pH 6.0 were 52.8%, 29.4%, 15.4%, respectively; these abilities decreased as pH increased. We conclude that Hominis Placenta Herbal Acupuncture may be useful as potential sources of antioxidant.

본 연구의 목적은 자하거약침액의 항산화기능에 관련한 생리활성을 보기 위하여 대한약침학회에서 제공받은 자하거 약침액을 시료로 사용하였다. 총 폴리페놀 함량은 자하거 약침액 원액에서 24.641 mg/L로 이는 산수유 등과 같은 약용식물과 비슷한 수준의 함량이다. 자하거약침액원액에서 DPPH radical 소거능과 Superoxide dismutase(SOD) 유사활성 측정은 각각 49.4%, 50.87%였다. 또한 ABTS radical 소거능과 Nitrite radical 소거능(pH1.5)에서는 각각 50.01%, 52.8%로 ABTS radical과 Nitrite radical 소거능이 우수하였다. 본 실험결과로볼 때, 자하거 약침액은 항산화능력은 뛰어난 것으로 사료된다. 하지만 현재 자하거 약침액과 관련된 실험이 부족한 실정이기 때문에 앞으로 이에 관련된 더 많은 연구가 필요하다고 생각된다.

Keywords

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