Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of Antimicrobial Dye for Natural Dyeing using Natural Substances

천연물을 이용한 항균성 염료의 개발

  • Yu, Young-Eun (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Park, Eun-Young (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Jung, Dae-Hwa (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Byun, Sung-Hui (College of Oriental medicine, Daegu Haany University) ;
  • Kim, Sang-Chan (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Park, Sung-Min (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University)
  • 유영은 (대구한의대학교 한방생명자원연구센터) ;
  • 박은영 (대구한의대학교 한방생명자원연구센터) ;
  • 정대화 (대구한의대학교 한방생명자원연구센터) ;
  • 변성희 (대구한의대학교 한의학과) ;
  • 김상찬 (대구한의대학교 한방생명자원연구센터) ;
  • 박성민 (대구한의대학교 한방생명자원연구센터)
  • Received : 2010.01.07
  • Accepted : 2010.03.13
  • Published : 2010.03.28
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Abstract

This study was carried out to investigate the usefulness of the natural antimicrobial substances for natural dyeing. Antimicrobial activity of natural substances, extracted by water, ethanol, methanol and ethyl acetate, were shown over 20 mm by Rheum palmatum, Caesalpinia sappan, Prunus mume, Schizandra chinensis, Rhus javanica, and Coptis japonica. Especially, water and methanol extract of C. japonica were shown strong antimicrobial activity against all investigated strains. Minimum inhibition concentration of C. japonica water extract was investigated 110 mg/mL for Staphylococcus aureus ATCC 6538. Investigated natural substances were not only shown strong antimicrobial activity for natural dyeing but also might be used other industries.

문헌조사를 바탕으로 천연 염색 시 염료로 사용이 기능할 것으로 판단되어지는 한약재와 천연물 중 72가지를 선발하고 메탄올 추출물을 이용한 1차 조사와 열수, 에탄올, 메탄올과 에틸아세테이트 추출물을 이용한 2차 조사 결과 전반적인 천연물에서 열수와 메탄올 추출물에서 상대적으로 유효한 항균성을 나타내는 것으로 확인되었다. 선별한 천연물 중 대황, 산사, 산수유, 소목, 오매, 오미자, 오배자와 황련 추출물에서 20 mm 이상의 생육저지환이 형성되었으나 항세균성에 대한 활성이 높은 것으로 조사되어 항진균성에 대한 연구가 보다 필요한 것으로 판단된다. 천연물 중 가장 우수한 항균성을 나타낸 황련 열수 추출물의 S. aureus ATCC 6538에 대한 MIC 값을 조사한 결과 110 mg/mL로 조사되었다. 또한 공시균주 외 다른 병원성 미생물에 대한 항균성을 조사한 결과 조사한 3종에 대하여도 양호한 항균성을 나타내는 것으로 조사되었다. 이에 선별된 천연물이 천연염색 뿐 아니라 다른 분야에서도 많이 적용될 수 있을 것으로 판단되었다. 그러나 용매에 따른 회수율과 항균성을 비교할 때 낮은 회수율에서도 높은 항균성을 나타내는 소목과 황련의 경우 회수율을 높일 수 있는 방법에 대한 연구가 진행된다면 보다 좋은 결과를 도출할 수 있을 것으로 판단되었다.

Keywords

References

  1. Ahn, J. S., H. J. Kim, K. H. Choi, B. G. Heo, and Y. J. Park. 2008. Effects of extract of Hibiscus mutabilis on dyeability of silk. J. Life Sci. & Nat. Res. 30: 55-60.
  2. Ahn, S. M., H. S. Jang, I. S. Kwun, and H. Y. Sohn. 2009. Evaluation of antimicrobial, antithrombin, and antioxidant activity of Aerial Bulbils of Dioscorea batatas Decne. Kor. J. Microbiol. Biotechnol. 37: 266-272.
  3. Bae, J. H. 2004. Antimicrobial effect of Fraxinus rhynchophylla extracts on food-borne pathogens. Kor. J. Microbiol. Biotechnol. 32: 277-281.
  4. Bae, J. H. 2005. Antimicrobial effect of Plagiorhegama dubium extract on food-borne pathogen. Korean J. Food & Nutr. 18: 81-87.
  5. Bae, J. H., M. J. Lee, and S. M. Lee. 2005. Antimicrobial effect of Cutellaria baicalensis George extracts on foodborne pathogens. Kor. J. Microbiol. Biotechnol. 33: 35-40.
  6. Bae, S. K. 2005. The dyeing propertyies of silk fabric with Brassica campestris. J. Kor. Soc. Cloth. Ind. 7: 542-546.
  7. Cai, H., S. I. Choi, Y. M. Lee, and T. R. Heo. 2002. Antimicrobial Effects of herbal medicine extracts on Staphylococcus aureus and Escherichia coli O157:H7. Korean J. Biotechnol. Bioeng. 17: 537-542.
  8. Choi, H., Y. S. Shin, C. N. Choi, S. Y. Kim, and Y. S. Chung. 2007. Dyeing properties and antimicrobial activity of soybean fiber with Gromwell Colorants. J. Kor. Soc. Cloth. Ind. 9: 119-123.
  9. Choi, K. E., J. S. Jeon, and S. I. Kang. 2004. A study on the dyeing of silk fabrics with Chelidonium Majus extracts. Journal of the Korea Home Economics Association. 42: 1-7.
  10. Chu, Y. J. 2002. The study on the dyeing properties of Rubia akane Nakai. Journal of the Korean Society of Clothing and Textiles. 26: 1301-1307.
  11. Han, M. R. and J. S. Lee. 2009. Natural dyeing of cotton fabrics with Tumex crispus L. Root. Journal of the Korea Home Economics Association. 33: 222-229.
  12. Hong, S. J., D. W. Jeon, J. J. Kim, and J. H. Jeon. 2005. Effect of chitosan and mordant treatments on the airpermeability, fastness and antimicrobial effect of cotton and nylon fabrics dyed using Rhusjara ica. The Research Journal of the Costume Culture. 13: 540-549.
  13. Jang, G. H., B. Y. Ahn, S. H. Oh, D. S. Choi, and Y. J. Kwon. 2000. Anticariogenic effects of Coptis chinensis Franch extract. Korean J. Food Sci. Technol. 32: 1396-1402.
  14. Jang, H. G., S. H. Kim, Y. J. Park, T. C. Kim, S. Y. Ahn, and B. G. Heo. 2004. Dyeability and antibacterial activity of the fabrics with Ficus carica extracts. Kor. J. Hort. Sci. Technol. 22: 130-134.
  15. Jung, D. S. and N. H. Lee. 2007. Antimicrobial activity of the Aerial part(leaf and stem) extracts of Cnidium officinale Makino, a korean medicinal herb. Kor. J. Microbiol. Biotechnol. 35: 30-35.
  16. Kim, A. S. 2004. A study on the chemical and dyeing properties of Petasites Japonicus leaf extract. Journal of the Korean Society of Clothing and Textiles. 28: 444-451.
  17. Kim, B. H. and H. S. Song. 2002. Dyeability and antimicrobiality of the silk fabric dyed with Inula britannica. Journal of the Korea Home Economics Association. 40: 99-105.
  18. Kim, T. Y. and J. D. Jang. 2008. Dyeing properties and antibacterial activity of wool blend fabrics dyed with Rhus Verniciflua extract. J. Kor. Soc. Cloth. Ind. 10: 106-112.
  19. Lee, H. and Y. U. Yu. 2004. Inhibitory effect of Caesalpinia sappan on caries - inducing properties of Streptococcus mutans and isolation of antibacterial component, brazilin. J. Wonkwang Dent. Res. Inst. 13: 63-83.
  20. Lee, S. H., Y. C. Lee, and S. K. Yoon. 2003. Isolation of the antimicrobial compounds from Omija (Achizandra chinensis) extract. Korean J. Food. Sci. Technol. 35: 483-487.
  21. Lee, S. P. and J. D. Moon. 2005. Dyeability and antibacterial activities of Maple leaves extracts. Journal of Korean Society of Color Studies. 19:13-19.
  22. Lee, S. K. 2003. Antimicrobial activity of Caesalpinia sappan against animal husbandry disease. Kor. J. Microbiol. Biotechnol. 31: 242-249.
  23. Oh, Y. S. and S. H. Lee. 2005. Isolation and identification of antimicrobial compound from Amarantus lividus. Kor. J. Microbiol. Biotechnol. 33: 123-129.
  24. Park, S. M., H. J. Jung, S. H. Han, S. H. Yeo, Y. W. Kim, H. G. Ahn, H. S. Kim, and T. S. Yu. 2005. Antifungal activity of extract from Xanthium strumarium L. against plant pathogenous fungi. Journal of Life Science. 15: 692-695. https://doi.org/10.5352/JLS.2005.15.5.692
  25. Park, Y. H. 2007. A study on the dyeability and antibiosis of fabrics dyed with Solanum nigrum extract. Jornal of the Korean Society of Costume. 57: 61-69.
  26. Park, Y. H. and H. J. Oh. 2003. The dyeability and antibacterial activity fabrics dyed with Chrysanthemum indicum L. extract. Jornal of the Korean Society of Costume. 53: 119-125.
  27. Park, Y. H., Y. J. Nam, and D. H. Kim. 2000. The study of antibiosis of the fabrics dyed by wormwood extract. Journal of the Korean Society of Clothing and Textiles. 24: 67-76.
  28. Seo, M. H. and J. H. Bae. 2002. Effect of butanol extracts from Prunus mume on the growth of Salmonella typhimurium. The Korean Nutrition Society. 35: 926-931.
  29. Shin, Y. S. and Y. J. Oh. 2002. Dyeing of cotton with Rosemary extract. Journal of the Korean Society of Clothing and Textiles. 26: 485-491.
  30. Son, D. H., S. I. Lee, and Y. G. Chung. 2001. Antioxidative of medicinal plants on pathogenic bacteria. J. Korean Soc. Hygienic Sciences. 7: 103-108.
  31. Yang, H. J., E. H. Kim, S. T. Kang, and S. N. Park. 2009. Antibacterial activity of Platycarya strobilacea extract and stability of the extract-containing cream. Kor. J. Microbiol. Biotechnol. 37: 170-175.