Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Effect of Astragali Radix and Opuntia humifusa on Quality of Red Ginseng Drink

황기 및 천년초 첨가가 홍삼음료의 품질에 미치는 영향

  • You, SangGuan (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Kim, Sung-Won (Eubio Healthcare Corp.) ;
  • Jung, Kyung-Hwan (Department of Biotechnology, Chungju National University) ;
  • Moon, Sung-Kwon (Department of Biotechnology, Chungju National University) ;
  • Yu, Kwang-Won (Department of Food Science and Technology, Chungju National University) ;
  • Choi, Won-Seok (Department of Food Science and Technology, Chungju National University)
  • 유상권 (강릉원주대학교 해양식품공학과) ;
  • 김성원 (주) 이유바이오) ;
  • 정경환 (충주대학교 바이오산업학과) ;
  • 문성권 (충주대학교 바이오산업학과) ;
  • 유광원 (충주대학교 식품공학과) ;
  • 최원석 (충주대학교 식품공학과)
  • Received : 2010.09.17
  • Accepted : 2010.11.12
  • Published : 2010.11.30
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Abstract

This study was performed to develop new functional red ginseng drinks with Astragali Radix and Opuntia humifusa. Optimum extraction conditions such as solvent property and temperature for Astragali Radix were determined by distilled water vs. ethanol (95%) ratio (0:100, 25:75, 50:50, 75:25) and 60 vs. $80^{\circ}C$. Water-soluble extracts at $80^{\circ}C$ showed higher antioxidant activities than fat-soluble extracts at $60^{\circ}C$. Viscosities of 1-2% (w/v) of Opuntia humifusa solution were similar to that of the 0.1% guar gum solution. Addtion of Astragali Radix (3% and 5%, w/v) and Opuntia humifusa (1.2%, w/v), especially, had effect on the changes of pH of the red ginseng solution(5%, w/v) during storage for 7 days. A significant difference during the storage was shown in total plate counts by addition of Opuntia humifusa (1.2%, w/v) and microorganisms were reduced by six log cycles. Significant antiproliferation effects of red ginseng (5%, w/v) solution with Astragali Radix (3% & 5%, w/v) and Opuntia humifusa (1.2%, w/v) on Colon26m-3.1 carcinoma (colorectal carcinoma) cell and U87-MG neuronale glioblastoma (brain carcinoma) cell were not observed.

본 연구에서는 홍삼음료의 다양화 및 기능성 강화와 여러 기능성을 가진 황기의 식품으로의 활용에 역점을 두고, 기존의 증점제를 대신하여 동결건조 천년초열매를 첨가한 새로운 기능성 홍삼음료을 개발하고자 하였다. 먼저 높은 항산화활성을 지닌 황기추출물을 얻고자 추출용매의 지용성정도를 달리하면서(100(증류수) : 0(95% 에탄올), 75 : 25, 50 : 50, 25 : 75) $80^{\circ}C$에서 추출한 추출물의 항산화활성을 측정한 결과, ABTS 및 DPPH radical 소거능 실험에서 증류수의 비율이 증가할수록 추출물의 항산화력이 증가하는 것으로 나타났다. 추출용매의 온도에 의한 황기추출물의 항산화활성 차이를 알아보고자 추출용액의 온도를 달리하면서(60, $80^{\circ}C$) 추출한 추출물의 항산화활성을 비교해 본 결과, $80^{\circ}C$에서의 추출물이 증류수와 에탄올혼합비율과는 상관없이 대부분 $60^{\circ}C$ 추출물보다 높은 값을 나타내었다. 기존의 점증제를 대신하여 다양한 기능성을 함유한 천연물 소재인 천년초분말을 선택하였으며, 구아검용액과 농도별 점도를 비교해 본 결과, 구아검 0.1%(w/v) 용액과 비슷한 점도를 나타내기 위해서는 천년초분말의 경우 1-2%(w/v)의 농도가 필요한 것으로 분석되었다. 황기와 천년초 첨가가 홍삼용액의 저장성($35^{\circ}C$)에 미치는 영향을 조사하기 위하여 이들 용액의 pH와 총미생물수를 7일 동안 측정하였다. 홍삼용액에 황기와 천년초를 첨가한 경우, 저장 1일째 부터 pH가 급격히 감소하기 시작하였으며, 저장 3일째에는 pH가 약 3.6까지 떨어진 다음, 저장 6일 이후에는 다소 pH가 증가하였으며, 천년초분말을 첨가한 용액에서는 황기의 농도증가에 의한 유의적 pH 감소는 나타나지 않았다. 천년초를 첨가한 홍삼용액의 경우 황기의 농도와는 상관없이 저장 7일째 미생물의 성장을 관찰할 수 없었으며, 천년초를 첨가하지 않은 시료에 비해 6 log cycle 이상의 큰 살균효과를 나타내었다. 황기(3, 5%, w/v), 천년초(1.2%, w/v)를 함유한 홍삼용액(5%, w/v)이 대장암 및 뇌종양세포 증식에 미치는 영향을 각각 0.5 mg/mL과 1 mg/mL의 농도에서 살펴본 결과, 종양세포에 대한 의미 있는 증식억제효과는 나타나지 않았다.

Keywords

Acknowledgement

Supported by : 식품의약품안전청

References

  1. Bae MJ, Kim KJ, Kim SJ, Ye EJ. 2007. Effect of mycelia extracts from Lentinus edodes mushroom-cultured Astragalus membranaceus bunge on anti-cancer and anti-allergy activities. J. Korean Soc. Food Sci. Nutr. 36: 8-13. https://doi.org/10.3746/jkfn.2007.36.1.008
  2. Baek NI, Kim YS, Kyung JS, Park KH. 1996. Isolation of antihepatotoxic agentfrom the root of Astragalus membranaceus. Kor. J. Pharnacogn. 27: 111-116.
  3. Cho EJ, Kim MJ, Choi WS. 2007. Quality properties of Jeungpyun with added withprickly pear powder. J. East Asian Soc. Dietary Life 17: 903-910.
  4. Cho Y, Choi MY. 2009. Quality characteristics of jelly containing added pomegranate powder and Opuntia humifusa powder. Korean J. Food Cookery Sci. 25: 134-142.
  5. Cho YJ, Chun SS, Lee KH, Kim JH, Kwon HJ, An BJ, Kim MU. 2006. Screening of the antimicrobial activity against Helicobacter pylori and antioxidant by extracts from Mulberry fruits. J. Korean Soc. Food Sci. Nutr. 35: 15-20. https://doi.org/10.3746/jkfn.2006.35.1.015
  6. Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura y, Ueno K. 1996. A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. Biol. Pharm. Bull. 19: 1518-1520. https://doi.org/10.1248/bpb.19.1518
  7. Jeon GJ, Han JY, Choi YM, Lee SM, Kim HT, Lee JS. 2008. Antioxidant and antiproliferative activity of pepper leaves. J. Korean Soc. Food Sci. Nutr. 37: 1079-1083. https://doi.org/10.3746/jkfn.2008.37.8.1079
  8. Jeong IY. 2005. Stability of functional properties and chemical components of gamma-irradiated Astragali Radix.J. Korean Soc. Food Sci. Nutr. 34: 255-260. https://doi.org/10.3746/jkfn.2005.34.2.255
  9. Jung IC, Park HS, Lee KS, Moon YH. 2008. Change in the quality of beef jerky containing additional pine needle or mugwort juice during storage. J. Life Sci. 18: 63-68. https://doi.org/10.5352/JLS.2008.18.1.063
  10. Kang BS, Kim DH, Whang HJ, Moon SW. 2006. The retrogradation of steamed korean rice cake with addition of gums. Korean J. Food Sci Technol. 38: 838-842.
  11. Kim JH, Mun YJ, Lee SW, Park JS, Woo WH. 2002. Effects of the butanol fraction of astragali radix on the cellular immune function in mice. Yakhak Hoeji 46: 52-57.
  12. Kim KT, Choi AR, Lee KS, Joung YM, Lee KY. 2007. Quality characteristics of bread made from domestic korean wheat flour containing catus chounnyouncho powder. Korean J. Food Cookery Sci. 23: 461-468.
  13. Kim SY. 2003. Studies on the separation of antioxidative and antimicrobial compounds of Korean perennial cactus cheonnyuncho. PhD thesis. Hoseo University. Asan. pp. 34-58.
  14. Kwon SC, Choi GH, Hwang JH, Lee KH. 2010. Physicochemical property and antioxidative activity of hot-water extracts from enzyme hydrolysate of Astragalus membranaceus. J. Korean Soc. Food Sci. Nutr. 39: 406-413. https://doi.org/10.3746/jkfn.2010.39.3.406
  15. Lee KS, Kim MG, Lee KY. 2004. Antimicrobial effect of the extracts of cactus chounnyouncho against food borne pathogens. J. Korean Soc. Food Sci. Nutr. 33: 1268-1272. https://doi.org/10.3746/jkfn.2004.33.8.1268
  16. Lee KS, Oh CS, Lee KY. 2005a. Antioxidative effect of the fractions extracted from a cactus chounnyouncho.Korean J. Food Sci. Technol. 37: 474-478.
  17. Lee SH, Hwang IG, Nho JW, Chang YD, Lee CH, Woo KS, Jeong HS. 2009. Quality characteristics and antioxidant activity of Chrysanthemum indicum L., Chrysanthemum boreals M. and Chrysanthemum zawadskii K. powdered teas. J. Korean Soc. Food Sci. Nutr. 38: 824-831. https://doi.org/10.3746/jkfn.2009.38.7.824
  18. Lee SO, Kim MJ, Kim DG, Choi HJ. 2005b. Antioxidative activities of temperature-stepwise water extracts from Inonotus obliquus. J. Korean Soc. Food Sci. Nutr. 34: 139-147. https://doi.org/10.3746/jkfn.2005.34.2.139
  19. Lee YS, Park DC, Joo EY, Shin SR, Kim NW. 2005c. Study on the antioxidant activity of the extracts from the Lepista nuda. J. Korean Soc. Food Sci. Nutr. 37: 942-947.
  20. Miller, HE. 1971. lified method for the evaluation of antioxidants. J. Am. Oil Chem. Soc. 48, 91.
  21. Park CS, Kim DH, Kim ML. 2008. Biological activities of extract from Corni fructus, Astragalus membranaceus and Glycyrrhiza uralensis. Kor. J. Herb. 23: 93-101.
  22. Park MK, Lee YJ. Kang ES. 2005. Hepatoprotective effect of chounnyouncho extract in rats treated carbon tetrachloride. Korean J. Food Sci. Technol. 37: 822-826.
  23. Park SM, Ahn IS, Hong SM, Kim DS, Kwon DY, Yang HJ. 2010. The effects of the supplementation of Opuntia humifusa water extracts and methyl sulfonyl methane on the laying productivity, egg quality and sensory characteristics. J. Korean Soc. Food Sci. Nutr. 39: 294-300. https://doi.org/10.3746/jkfn.2010.39.2.294
  24. Shin JK, Ha KY, Pyun YR, Choi MS, Chung MS. 2007. Pasteurization of carrot juice by high voltage pulsed electric fields with square wave pulse and quality change during storage. Korean J. Food Sci. Technol. 39: 506-514.
  25. Song BK, Lee EJ, Kim HK, Jin SD, Kim SJ, Kim DH. 1998. Effects of Astragali Radix on the fuction of murine immunocytes in Vivo and in Vitro. Korean. J. Herb. 13: 115-126.
  26. Yoon JA, Hahm SW, Park JE, Son YS. 2009. Total polyphenol and flavonoid of fruit extract of Opuntia humifusa and its inhibitory effect on the growth of MCF-7 human breast cancer cells. 2009. J. Korean Soc. Food Sci. Nutr. 38: 1679-1684. https://doi.org/10.3746/jkfn.2009.38.12.1679