Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Inhibitory Effects of Triticum aestivum L. Extracts on Liver Lipid Accumulation in High Fat-Fed Mice

고지방식이 섭취 마우스의 간 조직의 지질 축적에 대한 밀순 추출물의 억제 효과

  • Lee, Sun-Hee (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School) ;
  • Lim, Sung-Won (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School) ;
  • Lee, Young-Mi (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Seo, Joo-Won (Division of Bioscience and Bioinformatics, College of Natural Science and Research Center for Bio Food and Drug Materials, Myongji University) ;
  • Kim, Dae-Ki (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School)
  • 이선희 (전북대학교 의과대학 및 의과학연구소) ;
  • 임성원 (전북대학교 의과대학 및 의과학연구소) ;
  • 이영미 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 서주원 (명지대학교 자연과학대학 생명과학정보학부 및 농생명바이오식의약소재개발사업단) ;
  • 김대기 (전북대학교 의과대학 및 의과학연구소)
  • Received : 2011.10.31
  • Accepted : 2011.11.17
  • Published : 2011.12.31
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Abstract

Fatty liver disease refers to a range of disorders associated with fatty liver, which occur in excessive eating, evident infection or significant consumption of alcohol. This study was to investigate the effects of water and ethanol extracts of Triticum aestivum young leaf on lipid metabolism and accumulation in liver of mice fed with high-fat diet. Male C57BL/6 mice were divided into normal diet group, high fat diet (HFD) group, high fat diet group administrated with 200 mg/kg/day of T. aestivum water extract (HFD-TAWE) and high-fat group administrated with 200 mg/kg/day of T. aestivum ethanol extract (HFD-TAEE). TAWE and TAEE were administrated orally for 5 weeks once at the same time point. Both TAWE and TAEE significantly reduced body weight, food intake and liver tissue weight, which were augmented in high fat-fed mice. The serum levels of triglyceride, total and LDL-cholesterol also were significantly attenuated in both HFD-TAWE and HFD-TAEE groups compared to the HFD group. Moreover, administration of HFD-TAWE or HFD-TAEE reduced the lipid accumulation in liver tissue of mice fed with high fat diet. Levels of total lipids and triglyceride in liver tissues also was significantly reduced in HFDTAWE and HFD-TAEE groups compared to HFD group. The activities of serum ALT and AST revealed in HFD group were remarkedly decreased in HFD-TAEE groups. These results indicate that both water and ethanol extract of T. aestivum may improve the lipid accumulation in liver as well as lipid metabolism in serum, and that in particular, the ethanol extract of T. aestivum may has the potent anti-hyperlipidemic effect, suggesting that it may be a useful candidate for the therapy preventing fatty liver diseases.

Keywords

References

  1. Choi, M. S., Lee, M. K., Jung, U. J., Kim, H. J., Do, G. M., Park, Y. B. and Jeon, S. M. (2009) Metabolic response of soy pinitol on lipid-lowing, antioxidant and hepatoprotetic action in hamsters fed-high cholesterol diet. Mol. Nutr. Food Res. 53: 751-759. https://doi.org/10.1002/mnfr.200800241
  2. Cha, J. Y., Cho, Y. S. and Kim, D. J. (2001) Effect of chicory extract on the lipid metabolism and oxidative stress in rats. J. Kor. Soc. Food Sci. Nutr. 30: 1220-1226.
  3. Clark, J. M., Brancati, F. I. and Diehl, A. M. (2002) Nonalcoholic fatty liver disease. Gastroenterology 122: 1649- 1657. https://doi.org/10.1053/gast.2002.33573
  4. Cortez-Pinto, H., Camilo, M. E., Baptista, A., De Oliveira, A. G. and De Moura, M. C. (1999) Non-alcoholic fatty liver: another feature of the metabolic syndrome? Clin. Nutr. 18: 353-358. https://doi.org/10.1016/S0261-5614(99)80015-6
  5. Ludwig, J., Viggiano, T. R. and McGill, D. B. (1980) Nonalcoholic steatohepatitis: Mayo Clinic experiences with a hitherto unnamed disease. Mayo Clin. Proc. 55: 434-438.
  6. Marchesini, G., Bugianesi, E., Forlani, G., Cerrelli, F., Lenzi, M. and Manini, R. (2003) Nonalcoholic fatty liver, steatohepatitis, and the metabolic syndrome. Hepatology 37: 917- 923. https://doi.org/10.1053/jhep.2003.50161
  7. Day, C. P. and James, O. F. (1998) Steatohepatitis: a tale of two "hit"?. Gastroenterology 114: 842-845. https://doi.org/10.1016/S0016-5085(98)70599-2
  8. Schaffler, A., Scholmerich, J. and Buchler, C. (2005) Mechanism of disease: adipocytokines and visceral adipose tissue emerging role in nonalcoholic fatty liver disease. Nat. Clin. Pract. Gastroenterol. Hepatol. 2: 273-280. https://doi.org/10.1038/ncpgasthep0186
  9. Delgado, J. S. (2008) Evolving trends in nonalcoholic fatty liver disease. Eur. J. Int. Med. 19: 75-82. https://doi.org/10.1016/j.ejim.2007.02.034
  10. Park, S. H. (2008) Nonalcoholic steatohepatitis: Pathogenesis and treatment. Kor. J. Hepatol. 14: 12-27. https://doi.org/10.3350/kjhep.2008.14.1.12
  11. Hanninen, O., Rauma, A. L., Kaartinen, K. and Nenonen, M. (1999) Vegan diet in physiological health promotion. Acta Physiol. Hung. 86: 171-180.
  12. Nagaoka, H. (2005) Treatment of germinated wheat to increase levels of GABA and IP6 catalyzed by endogenous enzymes. Biotechnol. Prog. 21: 405-410.
  13. Ben-Arye, E., Goldin, E., Wengrower, D., Stamper, A., Kohn, R. and Berry, E. (2002) Wheat grass juice in the treatment of active distal ulcerative colitis: a randomized double blind placebo- controlled trial. Scand. J. Gastroenterol. 37: 444-449. https://doi.org/10.1080/003655202317316088
  14. Bonfili, L., Amici, M., Cecarini, V., Cuccioloni, M., Tacconi, R., Angeletti, M., Fioretti, E., Keller, J. N. and Eleuteri, A. M. (2009) Wheat sprout extract-induced apoptosis in human cancer cells by proteasomes modulation. Biochimie. 91: 1131- 1144. https://doi.org/10.1016/j.biochi.2009.06.001
  15. Tudek, B., Peryt, B., Mi oszewska, J., Szymczyk, T., Przybyszewska, M. and Janik, P. (1998) The effect of wheat sprout extract on benzo(a)pyrene and 7,2-dimethylbenz(a)anthracene activity. Neoplasma 35: 515-523.
  16. Watzl, B. (2008) Anti-inflammatory effects of plant-based foods and of their constituents. Int. J. Vitam. Nutr. Res. 78: 293-298. https://doi.org/10.1024/0300-9831.78.6.293
  17. Eddouks, M., Maghrani, M. and Michel, J. B. (2005) Hypoglycaemic effect of Triticum repens P. Beauv. in normal and diabetic rats. J. Ethnopharmacol. 102: 228-232. https://doi.org/10.1016/j.jep.2005.06.019
  18. Lee, S. H., Lee, Y. M., Lee, H. S. and Kim, D. K. (2009) Anti-oxidative and anti-hyperglycemia effect of Triticum aestivum wheat sprout water extracts on the streptozotocininduced diabetic mice. Kor. J. Pharmacogn. 40: 408-414.
  19. Lindstrm, P. (2007) The physiology of obese-hyperglycemic mice [ob/ob mice]. Sci. World J. 7: 666-685. https://doi.org/10.1100/tsw.2007.117
  20. Lee, S. H., Lim, S. W., Lee, Y. M., Lee, H. S. and Kim, D. K. (2011) Administration of Triticum aestivum sprout Water Extracts regulate the level of Blood Glucose and Lipid in Leptin Deficient ob/ob Mice. J. Kor. Soc. Food Sci. Nutr. 40: 401-408. https://doi.org/10.3746/jkfn.2011.40.3.401
  21. Friedewald, W., Levy, R. and Fredrickson, D. (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18: 499-502.
  22. Folch, J., Mee, L. and Stanley, G. S. H. (1975) A simple method for the isolation and purification of total lipid from animal tissues. J. Biol. Chem. 226: 497-509.
  23. Frings, C. S. and Dunn, R. T. (1970) A colorimetric method for determination of total serum lipids based on the sulfophospho- vanillin reaction. Am. J. Clin. Pathol. 53: 89-91.
  24. Hong, H. S., Ryu, H. K. and Kim, W. Y. (2008) The association of plasma HDL-Cholesterol level with cardiovascular disease related factors on korean type 2 diabetic patients. J. Kor. Diabet. 32: 215-223. https://doi.org/10.4093/kdj.2008.32.3.215
  25. Rim, J. C. K. and Kang, S. A. (2001) Effect of high fat and high carbohydrate diet on serum leptin and lipids concentration in rats. Kor. J. Nutr. 34: 123-131.
  26. Ministry of Health and Social Affairs. (2008) 2008 national health and nutrition survey: overview. Ministry of health and Welfare, Seoul, Korea. 272-275.
  27. National Cholesterol Education Program Expert Panel. (2002) Third Report of the National Cholesterol Education Program Expert Panel on Detection, Evaluation and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III) final report. Circulation 106: 3143-3421.
  28. Friedman, S. F., Martin, P. and Munoz, J. S. (2003) Laboratory evaluation of the patient with liver disease. Hepatology, a textbook of liver disease. Philedelphia; Saunders publication 1: 661-709.
  29. Burkus, Z. and Temelli, F. (1998) Effect of extraction conditions on yield, composition and viscosity stability of barley $\beta$ gum. Cereal Chern. 75: 805-809. https://doi.org/10.1094/CCHEM.1998.75.6.805
  30. Lee, Y. T., Yoo, J. W., Yoo, M. S., Choi, K. H., Kim, J. H. and Seog, M. H. (2003) Retarding effects of $\beta$-glucans separated from barley bran on in vitro transport of bile acid and glucose. Food Sci. Biotechnol. 12: 298-302.
  31. Lee, S. H., Lee, Y. M., Lee, H. S. and Kim, D. K. (2010) Anti-diabetic Effects of Triticum aestivum L. Water Extracts in db/db Mice as an Animal Model of Diabetes Mellitus Type II. Kor. J. Pharmacogn. 41: 282-288.
  32. Yang, E. J., Cho, Y. S., Choi, M. S., Woo, M. N., Kim, M. J., Shon, M. Y. and Lee, M. K. (2009) Effect of Young Barley Leaf on Lipid Contents and Hepatic Lipid-Regulating Enzyme Activities in Mice Fed High-Fat Diet. Kor. J. Nutr. 42: 14-22.
  33. Won, H. R., Lee, S. G. and Park, D. Y. (2005) Effects of hot water soluble extract from green tea on the lipid metabolism and antioxidant effect in rats fed animal or vegetable protein and a hypercholesterol diet. Kor. J. Comm. Living Sci. 16: 39- 45.
  34. Yugarani, T., Tan, B. K., The, M. and Das, N. P. (1992) Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets. Lipids 27: 181-186. https://doi.org/10.1007/BF02536175
  35. Wang, J. Q., Li, J., Zou, Y. H., Cheng, W. M., Lu, C., Zhang, L., Ge, J. F., Huang, C., Jin, Y., Lv, X. W., Hu, C. M. and Liu, L. P. (2009) Preventive effects of total flavonoids of Litsea coreana leve on hepatic steatosis in rats fed with high fat diet. J. Ethnopharmacol. 121: 54-60. https://doi.org/10.1016/j.jep.2008.09.029