DOI QR코드

DOI QR Code

Characteristics and Antioxidant Activities of Rehmanniae radix Powder

지황 및 숙지황 분말의 품질 및 항산화 특성

  • Oh, Hye Lim (Dept. of Food & Nutrition, Chungnam National University) ;
  • Kim, Cho Rong (Dept. of Food & Nutrition, Chungnam National University) ;
  • Kim, Na Yeon (Dept. of Food & Nutrition, Chungnam National University) ;
  • Jeon, Hye Lyun (Dept. of Food & Nutrition, Chungnam National University) ;
  • Doh, Eun Soo (Dept. of Oriental Pharmaceutical Science, Joongbu University) ;
  • Kim, Mee Ree (Dept. of Food & Nutrition, Chungnam National University)
  • 오혜림 (충남대학교 식품영양학과) ;
  • 김초롱 (충남대학교 식품영양학과) ;
  • 김나연 (충남대학교 식품영양학과) ;
  • 전혜련 (충남대학교 식품영양학과) ;
  • 도은수 (중부대학교 한방제약과학과) ;
  • 김미리 (충남대학교 식품영양학과)
  • Received : 2012.08.20
  • Accepted : 2012.12.13
  • Published : 2013.01.31

Abstract

This study was carried out to investigate the quality characteristics and antioxidant activities of Rehmanniae radix (Rehmannia glutinosa Libosch. var. purpurea Makino) freeze-dried powder (RRP) and Rehmanniae radix preparata powder (RRPP). Under the Hunter color system, redness was higher and lightness/yellowness lower in RRPP, compared to RRP. The reducing sugar contents of RRP and RRPP were 0.8% and 6.0%, respectively (p<0.05). The pH was lower in the RRPP (RRP: 6.71, RRPP: 4.23). The amount of catalpol amount in RRPP (47.20 mg/mL) was lower than RRP (144.90 mg/mL). RRPP contained high amounts of 5-HMF (5-hydroxymethyl-2-furaldehyde, 47,231 mg/mL), but 5-HMF was not detected in RRP. Total phenol contents of RRP and RRPP were 2.10 mg/mL and 3.66 mg/mL, respectively. FRAP values of RRP and RRPP were 0.51 mg/mL and 1.99 mg/mL, respectively. The antioxidant activities by DPPH (1,1-diphenyl-2-picryl hydrazyl) and hydroxyl radical scavenging activity of RRPP were much higher than RRP. Based on these results, RRPP is a good candidate for food processing in terms for its physicochemical and antioxidative activities.

생리활성이 높은 지황을 식품소재로 간편하게 활용할 수 있도록 하기 위한 연구로, 생지황과 숙지황을 동결건조한 후 이화학적 특성과 항산화능 분석 및 관능검사를 실시하였다. 명도와 황색도는 숙지황 분말이 숙지황 분말보다 낮았고, 적색도는 숙지황 분말이 생지황 분말보다 높았다. 당도 및 환원당 함량은 숙지황 분말이 $30.3^{\circ}Brix$ 및 6.06%로 생지황분말에 비하여 높았다. pH는 숙지황 분말은 4.23으로 생지황 분말의 6.71보다 낮았으며 산도는 숙지황 분말은 0.35%로 생지황 분말의 0.20%보다 높았다. 일반성분 분석에서 수분함량은 숙지황 분말 4.2%, 지황분말 3.8%로 숙지황 분말에서 높았으나 그 외 일반성분은 유사하였다. 유효지표성분 분석에서 catalpol 함량은 생지황 분말에서 144.96 mg/mL로 검출되었고 숙지황 분말에서는 47.20 mg/mL로 검출되었다. 5-HMF는 숙지황 분말에서만 검출되었다. 총 phenol 함량 측정 결과 생지황 분말이 2.09 mg/mL, 숙지황 분말이 3.66 mg/mL로 생지황 분말보다 숙지황 분말에서 현저히 높은 함량을 나타내었고 DPPH radical 소거능 측정 결과 $IC_{50}$ 값이 생지황 분말 26.64 mg/mL, 숙지황 분말 13.74 mg/mL로 숙지황 분말의 항산화능이 매우 높았다. Hydroxyl radical 소거능 $IC_{50}$ 값은 숙지황 분말의 값이 6.37 mg/mL로 생지황 분말의 13.27 mg/mL보다 더 낮게 나타나 숙지황 분말의 항산화능이 더 높게 나타났다. FRAP 활성 측정 결과 생지황 분말이 0.51 mg/mL, 숙지황 분말이 1.99 mg/mL로 숙지황 분말이 높아 전반적으로 숙지황 분말의 항산화능이 더 높았다. 이상의 결과로부터 숙지황 분말이 식품소재로 더 적당한 것으로 사료된다.

Keywords

References

  1. Hong SP, Kim YC, Kim KH, Park JH, Park MK. 1993. Characteristic component of Rehmanniae radix Preparata compared to Rehmanniae radix and Rehmanniae radix Crudus. J Korean Soc Anal Sci 6: 401-404.
  2. Lee CK, Seo JM. 2004. Changes of the constituents in the Rehmanniae radix Preparata during processing. J Korean Soc Food Sci Nutr 33: 1748-1752. https://doi.org/10.3746/jkfn.2004.33.10.1748
  3. Shih CK, Son YJ, Lee YJ. 1999. Changes in the carbohydrate contents of Rehmanniae Radix during processing. Kor J Herbology 14: 1-11.
  4. Lee JH, Koh JA, Hwang EY, Hong SP. 2002. Quantitative determination of 5-hydroxymethyl-2-furaldehyde from Rehmanniae Radix Preparata according to various processings. Kor J Herbology 17: 145-149.
  5. Park SJ, Park HS, Yoo SO. 1998. Effects of supplemention of Rehmannia radix on performance and physiological status in broiler chicks. Korean J Poult Sci 25: 195-202.
  6. Lee SI, Ahn DK, Shin MK, No SH, Lee YJ, Kim SH. 1986. Oriental Medical Application. Sungbosa, Seoul, Korea. p 354-355.
  7. Jung BS, Shin MK. 1998. Illustration crude drug unabridged dictionary. Youngrimsa, Seoul, Korea. p 906-909.
  8. Tomoda M, Miyamoto H, Shimizu N. 1994. Structural features and anti-complementary activity of rehmannan SA, a polysaccharide from the root of Rehmannia glutinosa. Chem Pharm Bull (Tokyo) 42: 1666-1668. https://doi.org/10.1248/cpb.42.1666
  9. Chen LZ, Feng XW, Zhou JH. 1995. Effects of Rehmannia glutinosa polysaccharide b on T-lymphocytes in mice bearing sarcoma 180. Zhongguo Yao Li Xue Bao 16: 337-340.
  10. Tomoda M, Miyamoto H, Shimizu N, Gonoda R, Ohara N. 1994. Characterization of two polysaccharides having activity on the reticuloendothelial system from the root of Rehmannia glutinosa. Chem Pharm Bull 42: 625-629. https://doi.org/10.1248/cpb.42.625
  11. Tomoda M, Miyamoto H, Shimizu N, Gonoda R, Ohara N. 1994. Two acidic polysaccharides having reticuloendothelial system-potentiating activity from the raw root of Rehmannia glutinosa. Biol Pharm Bull 17: 1456-1459. https://doi.org/10.1248/bpb.17.1456
  12. Yang JY. 2007. The risk assessment of hazardous materials in Rehmanniae Radix Preparata. MS Thesis. Daegu Hanny University, Gyeongbuk, Korea.
  13. Brodbeck U. 1980. Enzyme inhibitors. Verlag Chemie, Weinheim, Germany. p 109-112.
  14. Gray GM. 1975. Carbohydrates digestion and absorption-. role of the small intestine. N Eng J Med 292: 1225-1230. https://doi.org/10.1056/NEJM197506052922308
  15. Ahn DK, Kim CM, Shin MK, Lee KS. 1998. Oriental Medicine Dictionary. Chungdam, Seoul, Korea. p 168-176.
  16. Hwang SY, Hwang BY, Choi WH, Jung HJ, Huh JD, Lee KS, Ro JS. 2001. Quantitative determination of 5-hydroxymethyl- 2-furaldehyde in the Rehmanniae Radix Preparata samples at various processing stages. Kor J Pharmacogn 32: 116-120.
  17. Park NK, Kim SL, Hur HS, Park CH. 2002. Development of R. Radix preparata with new variety "Jiwhang 1". Kor J Intl Agri 14: 34-39.
  18. You BR, Kim HR, Kim HJ, Lee JY, Lee SY, Song MR, Park JY, Kim MR. 2011. Catalpol content and antioxidant activities in various cultivars of Rehmannia glutinosa. J Korean Soc Food Sci Nutr 40: 481-485. https://doi.org/10.3746/jkfn.2011.40.4.481
  19. AOAC. 1995. Official methods of analysis. 15th ed. Association of Official Analytical Chemists, Arington, VA, USA.
  20. Korean Food Standards Codex. 2007. Korea Food & Drug Administration, Chungwon, Korea.
  21. Park BY, Chang SM, Choi J. 1989. Effects of soil properties on the sontents of satalpol, sugars and ash in the Rhizoma of Rehmannia glutinosa. J Korean Agric Chem Soc 32:240-248.
  22. Singleton VL, Rossi Jr JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitric 16: 144-158.
  23. Benzie IF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 230: 70-76.
  24. Song DS, Woo KS, Seong NS, Kim KY, Jeong HS, Lee HB. 2007. Changes in Quality of Rehmanniae radix Preparata with heating conditions. J Korean Soc Food Sci Nutr 36:773-778. https://doi.org/10.3746/jkfn.2007.36.6.773
  25. Hong HD, Kim YC, Rho JH, Kim KT, Lee YC. 2007. Changes on physicochemical properties of Panax ginseng C.A. Meyer during repeated steaming process. J Ginseng Res 31: 222-229. https://doi.org/10.5142/JGR.2007.31.4.222
  26. Oh HL, You BR, Kim HJ, Lee JY, Kim NY, Song JE, Kim MR. 2011. Quality characteristics and antioxidant activities of Rhmanniae radix paste. J Korean Soc Food Sci Nutr 40: 1518-1524. https://doi.org/10.3746/jkfn.2011.40.11.1518
  27. Choi DJ, Lee SJ, Kang MJ, Cho HS, Sung NJ, Shin JH. 2008. Physicochemical characteristics of black garlic (Allium sativum L.). J Korean Soc Food Sci Nutr 37: 465-471. https://doi.org/10.3746/jkfn.2008.37.4.465
  28. Choi JH, Kim WJ, Park KD, Sung HS. 1980. Color evaluation of red ginseng extract and its changes during heat treatment. Korean J Ginseng Sci 4: 165-174.
  29. Kim RJ, Kang MJ, Lee SJ, Shin JH, Sung NJ. 2010. Physicochemical characteristics and antioxidant activities of fermented garlic husk. J Korean Soc Food Sci Nutr 39:1731-1738. https://doi.org/10.3746/jkfn.2010.39.12.1731
  30. Park CY, Lim JI, Ryu KS. 1987. Content of catalpol in Rehmanniae Radix and its preparations. Bull K H Pharma Sci 15: 93-98.
  31. Ahn SW, Kim YG, Kim MH, Lee HY, Seong NS. 1999. Comparison of biological activities on Rehmannia radix and R. Radix Preparata produced in Korea. Korean J Medicinal Crop Sci 7: 257-262.
  32. Jiratanan T, Liu RH. 2004. Antioxidant activity of processed table beets (Beta vulgaris var, conditiva) and green beans (Phaseolus vulgaris L.). J Agric Food Chem 52: 2659-2670. https://doi.org/10.1021/jf034861d
  33. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J Food Sci Technol 36: 333-338.
  34. You JK, Chung MJ, Kim DJ, Seo DJ, Park JH, Kim TW, Choi M. 2009. Antioxidant and tyrosinase inhibitory effects of Paeonia suffruticosa water extract. J Korean Soc Food Sci Nutr 38: 292-296. https://doi.org/10.3746/jkfn.2009.38.3.292
  35. Jung LS, Yoon WB, Park SJ, Park DS, Ahn JH. 2012. Evaluation of physicochemical properties and biological activities of steamed and fermented Deodeok (Codonopsis lanceolata). Korean J Food Sci Technol 44: 135-139. https://doi.org/10.9721/KJFST.2012.44.1.135
  36. Lee JW, Do JH. 2006. Current studies on browning reaction products and acidic polysaccharide in Korean red ginseng. J Ginseng Res 30: 41-48. https://doi.org/10.5142/JGR.2006.30.1.041
  37. Kim HJ, Lee JY, You BR, Kim HR, Choi JE, Nam KY, Moon BD, Kim MR. 2011. Antioxidant activities of ethanol extracts from black ginseng prepared by steaming-drying cycles. J Korean Soc Food Sci Nutr 40: 156-162. https://doi.org/10.3746/jkfn.2011.40.2.156
  38. Lee JY, Kim NY, Oh HL, Lee KJ, Yang KH, Doh ES, Kim MR. 2011. Antioxidant activity of Rehmanniae Radix preparata prepared from dried root through steaming-drying cycles. J East Asian Soc Dietary Life 21: 838-843.

Cited by

  1. Antioxidant Activities and Quality Characteristics of Bread Added with Dried Mulberry Pomace vol.29, pp.6, 2013, https://doi.org/10.9724/kfcs.2013.29.6.769
  2. Quality Characteristics and Antioxidative Activities of Rice Cookies with Rehmannia glutinosa Preparata vol.31, pp.2, 2015, https://doi.org/10.9724/kfcs.2015.31.2.136
  3. Quality Characteristics and Antioxidant Activities of Noodles Added with Rehmanniae Radix Preparata Powder vol.44, pp.3, 2015, https://doi.org/10.3746/jkfn.2015.44.3.386
  4. Quality Characteristics and Antioxidant Activities of Rehmannia glutinosa JungKwa Prepared with Different Kinds of Sugars vol.30, pp.1, 2014, https://doi.org/10.9724/kfcs.2014.30.1.076
  5. 가미도홍사물탕(加味桃紅四物湯)이 HUVEC cell 내에 eNOS, KLF2, ICAM-1, VCAM-1의 유전자 발현양에 대해 미치는 영향 vol.39, pp.1, 2013, https://doi.org/10.22246/jikm.2018.39.1.1
  6. Quality characteristics and antioxidant activities of aged black Liriope platyphylla vol.26, pp.5, 2013, https://doi.org/10.11002/kjfp.2019.26.5.505
  7. 지황의 출아조건 및 플러그 육묘기간 구명 연구 vol.27, pp.4, 2013, https://doi.org/10.7783/kjmcs.2019.27.4.271
  8. Effects of Bread Added with psyllium Husk Powder on Aging during Storage vol.30, pp.4, 2020, https://doi.org/10.17495/easdl.2020.8.30.4.261
  9. 매실 식초 분말의 항산화 및 유방암 세포주 증식 억제 효과 vol.31, pp.2, 2013, https://doi.org/10.5352/jls.2021.31.2.149
  10. A Study on Improvement of the Analytical Method of Chlorantraniliprole Residue in Herbal Medicine (Rehmannia glutinosa Libosch) using HPLC-UVD vol.25, pp.3, 2013, https://doi.org/10.7585/kjps.2021.25.3.196