Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Anti-inflammatory and Tyrosinase Inhibition Effects of Amaranth (Amaranthus spp L.) Seed Extract

아마란스 씨앗 추출물의 항염 및 Tyrosinase 억제 효과

  • Yi, Mi-Ran (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Kang, Chang-Hee (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Bu, Hee-Jung (Biotechnology Regional Innovation Center, Jeju National University)
  • 이미란 (제주대학교 생명과학기술혁신센터) ;
  • 강창희 (제주대학교 생명과학기술혁신센터) ;
  • 부희정 (제주대학교 생명과학기술혁신센터)
  • Received : 2017.01.31
  • Accepted : 2017.04.14
  • Published : 2017.04.30
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Abstract

This study examined the anti-inflammatory and whitening effects of Amaranth (Amaranthus spp L.) seed extract. Amaranthus spp L. seeds were extracted using 70% ethanol and then fractionated sequentially with n-hexane, dichloromethan, ethyl acetate and butanol. For the study of anti-inflammatory activity in RAW 264.7 cells, EtOAc fraction of Amaranthus spp L. seeds significantly inhibited nitrogen oxide production as well as the protein level of iNOS. Furthermore, EtOAc fraction of Amaranthus spp L. seeds inhibited expression of $TNF-{\alpha}$, PGE2 and the protein level of COX-2 in a dose-dependent manner. Inaddition, the tyrosinase inhibitory activities of the Amaranthus spp L. seed 70% ethanol extract and subfractions were also measured to see if these extracts can be used as an ingredient for whitening cosmetics. Tyrosinase is an oxidase that is a rate-limiting enzyme for controlling the production of melanin. Therefore, tyrosinase inhibitors have become increasingly important in cosmetics and medical products with regards to hyperpigmentation. EtOAc fraction of Amaranthus spp L. seeds showed mushroom tyrosinase inhibitory activity in a dose-dependent manner. This activity was more potent than that of a positive control cynandione A. These results suggest that Amaranthus spp L. seeds may be a valuable natural ingredient for the food and cosmetics industries.

본 연구는 아마란스(Amaranthus spp L.) 씨앗 추출물의 항염 및 미백과 관련된 효능을 측정함으로써 식품, 의약품 및 화장품 등에 유용한 소재로서 활용할 수 있는 가능성을 확인하고자 수행하였다. 아마란스(Amaranthus spp L.) 씨앗 추출물의 항염증 활성 검색을 위하여 RAW 264.7 세포를 이용하고 실험을 진행하였고, 미백 효능 확인에 대하여서는 mushroom tyrosinase를 사용하였다. RAW 264.7 세포를 이용하여 NO 생성 억제, iNOS 및 COX-2 단백질 발현 억제, 염증매개인자인 $TNF-{\alpha}$및 $PGE_2$ 생성 억제효과 등을 확인한 결과, 에틸아세테이트 분획물이 NO 생성과 iNOS 발현을 농도 의존적으로 강하게 억제함으로써 iNOS 발현 억제를 통한 NO 생성을 억제함을 확인할 수 있었다. NO와 iNOS 억제 효과보다는 약하지만 $PGE_2$와 COX-2 단백질 발현 역시 농도 의존적으로 억제함으로써 $PGE_2$ 억제 효능이 COX-2의 발현 억제를 통하여 이루어짐을 확인하였다. 또한 염증에 의해 증가되는 아라키돈산 대사물들이 피부 멜라닌 합성의 최초 속도결정단계에 작용하는 tyrosinase 활성을 증가시킨다는 연구 결과에 근거하여 $PGE_2$의 발현을 억제하는 아마란스 EtOAc 분획물이 tyrosinase 효소 억제 활성을 측정하고 미백효능을 확인하였다. Mushroom tyrosinase 활성 억제 효능은 에틸아세테이트 분획물이 우수한 효과를 보였으며, 농도 의존적으로 tyrosinase 효소의 활성을 억제함을 확인할 수 있었다. 이상의 결과들은 향후 아마란스 씨앗의 항염 효능을 갖는 유효성분 탐색 및 멜라닌 세포를 이용하여 미백 관련 연구의 중요한 기초자료로 활용할 수 있을 것이라 사료되며, 아마란스 씨앗이 우수한 영양식품으로 알려져 있는 만큼 안전하면서도 항염과 미백 효능이 있는 소재로서 기능성 식품, 의약품, 화장품 소재로 다양하게 응용할 수 있는 가능성을 확인하였다.

Keywords

References

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