The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Characteristics of Unrecorded Wild Yeasts Obtained from the Soil of Spices Plant Fields and its Physiological Functionality

향신료 재배 토양으로부터 분리한 국내 미기록 야생효모들의 균학적 특성 및 생리활성

  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kim, Ji-Yoon (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kim, Changmu (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 한상민 (배재대학교 바이오 의생명공학과) ;
  • 김지윤 (배재대학교 바이오 의생명공학과) ;
  • 김창무 (국립생물자원관 미생물자원과) ;
  • 이종수 (배재대학교 바이오 의생명공학과)
  • Received : 2018.11.26
  • Accepted : 2019.01.15
  • Published : 2019.03.01
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Abstract

The goal of this study was to characterize unrecorded wild yeasts from soils of spices plants fields and further, to elucidate its anti-demential activities and tyrosinase inhibitory activity. Piskurozyma taiwanensis R4-1 (NIBRFGC000502619), Nadsonia starkeyi-henricii R6-2 (NIBRFGC000502618), and Canadida friedrichii M12-6 (NIBRFGC000502615) isolated from soil of garlic field represented newly recorded yeast strains in Korea. Vishniacozyma peneaus I2-9 (NIBRFGC000502617) and Cryptococcus aspenensis I21-1 (NIBRFGC000502616) from soil of ginger field represented also newly recorded yeast strains, and microbiological characteristics of its fifteen yeast strains were investigated. All of these unrecorded yeasts exhibited oval-global shape and have ascospores except Canadida friedrichii M12-6. Piskurozyma taiwanensis R4-1 and Canadida friedrichii M12-6 grew well in vitamin-free medium, and Piskurozyma taiwanensis R4-1 was halotolent growing in 10% NaCl-containing yeast extract peptone dextrose (YPD) broth. After prepared cell-free of the unrecorded wild yeasts, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities as anti-dementia activity and tyrosinase inhibitory activity as whitening activity were determined. Cell-free extract from Canadida friedrichii M12-6 had the highest tyrosinase inhibitory activity of 14.4%.

생리기능성을 조사하기 위하여 먼저 충남 금산의 양파와 도라지, 마늘과 생강 등의 재배지 토양시료에서 분리, 동정한 야생효모 중 마늘 재배지에서 분리한 Piskurozyma taiwanensis R4-1 (NIBRFGC000502619), Nadsonia starkeyi-henriciiR6-2 (NIBRFGC000502618), Canadida friedrichiiM12-6 (NIBRFGC000502615) 균주와 생강에서 분리한 Vishniacozyma peneaus I2-9 (NIBRFGC000502617)와 Cryptococcus aspenensisI21-1 (NIBRFGC000502616) 균주의 야생효모를 국내 미기록 효모 균주로 최종 선별하여 이들의 형태학적, 배양학적 특성 등을 조사하였다. 미기록 균주들은 구형~타원형으로 PiskurozymataiwanensisR4-1, Nadsoniastarkeyi-henriciiR6-2들은 자낭포자를 형성하였다. Vishniacozyma peneausI2-9, Cryptococcus aspenensisI21-1, Piskurozyma taiwanensis R4-1, Nadsonia starkeyi-henricii R6-2는 yeast extract-peptone-dextrose (YPD) 배지와 yeast extract-malt extracts (YM) 배지, 비타민을 첨가하지 않은 YPD 배지에서도 모두 생육하였으며 Vishniacozyma peneausI2-9 외 4균주 모두 10% NaCl을 함유한 YPD 배지에서 생육하는 호염성 효모였다.

Keywords

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Fig. 1. Phylogenetic tree of unrecorded yeasts isolated from the soil of spice plant fields of Geumsan, Chungnam province, Korea, based on the nucleotide sequences of large subunit 26S ribosomal DNA D1/D2 region. The tree was generated by the neighbor-joining method, using MEGA7.

Table 1. Microbiological characteristics of unrecorded wild yeasts from the soil of spice plant felds of Geumsan, Chungnam province, Korea

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Table 2. Several physiological funtionalities of unrecorded wild yeasts from soils of spices plants felds of Geumsan, Chungnam province, Korea

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