The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation of Wild Yeasts Obtained from Waters and Soils of Riversides in Sangjubo of Nakdong River and Daechung Dam of Geumgang Midstream, Korea and Characterization of Unrecorded Wild Yeasts

낙동강 상주보와 금강중류 대청댐 주변으로부터 야생효모의 분리 및 국내 미기록 효모들의 균학적 특성

  • Han, Sang-Min (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Park, Dong-Jae (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Kim, Ji-Yoon (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Lim, Hyo Jin (Division of Food Technology, Biotechnology and Agrochemistry, Chonnam National University) ;
  • Lee, Hyang Burm (Division of Food Technology, Biotechnology and Agrochemistry, Chonnam National University) ;
  • Lee, Jong-Soo (Faculty of Biomedicine and Biotechnology, Paichai University)
  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 박동재 (배재대학교 바이오.의생명공학과) ;
  • 김지윤 (배재대학교 바이오.의생명공학과) ;
  • 임효진 (전남대학교 응용생물공학부) ;
  • 이향범 (전남대학교 응용생물공학부) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Received : 2020.05.15
  • Accepted : 2020.09.16
  • Published : 2020.09.30
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Abstract

The goal of this study was to isolate wild yeasts from waters and soils in Sangjubo of Nakdong river and Daechung dam in Daejeon city, Korea and investigate characteristics of previously unrecorded novel wild yeasts. In total, 94 strains from 39 different species of wild yeasts were isolated from 47 soils and waters samples obtained from Sangjubo in Nakdong river. Among these, 3 strains, including Rhodosporidium azoricum JSL-GRU-009, represented novel yeast strains which were previous recorded. Two hundred nine strains from 105 different species of wild yeasts were isolated from 130 soils and water samples obtained from Daechungdaegyo and Daechung dam of Shintanjin in Daejeon city, Korea. Ten strains including Bullera pseudoalba JSL-GRU-005 also represented newly recorded strains in Korea. All the 13 previously unrecorded yeasts were oval or global in shape, and nine strains, including R. azoricum JSL-GRU-009, formed ascospores. Ten strains grew well in vitamin-free medium. R. azoricum JSL-GRU-009 grew well in 20% glucose-containing yeast extract-peptone-dextrose (YPD) medium and 3 strains, including Rhodotorula oryzicola JSL-GRU-003, grew well in 5% NaCl-containing YPD medium.

경상북도 낙동강의 상주보와 금강중류인 대전시 신탄진의 대청댐주변의 야생효모들의 종 분포특성을 알아보기 위해 먼저 이들지역의 토양과 물들로부터 야생효모들을 분리, 동정하였다. 낙동강의 상주보에서 채취한 시료 47점에서 모두 39종 94 균주의 야생효모들이 분리, 동정되었고 Cryptococcus속 균들이 가장 많았다. 이들중Rhodosporidium azoricum JSL-GRU-009를 포함한 3균주들이 아직까지 국내에 보고되지 않은 미기록 효모들로 최종 선별되었다. 또한, 금강중류지역인 대전시 신탄진의 대청대교와 대청댐주변 토양과 물등 130종의 시료들로부터 105종 209균주들이 분리, 동정되었고 이들 야생효모 균주들로부터 Bullera pseudoalba JSL-GRU-005를 포함한 10종의 균들이 국내 미기록 효모들로 최종 선별되었다. 이들 낙동강 상주보와 대청댐등에서 선별된 13균주들의 균학적 특성을 조사한 결과 이들 미기록 균주들은 구형~타원형으로 출아, 분열법으로 영양 증식하였다. R. azoricum JSL-GRU를 포함한 9균주들이 포자를 형성하였고 10균주들이 비타민을 첨가하지 않은 YPD 배지에서 생육하였다.R. azoricum JSL-GR균주는 20% 포도당을 함유한 YPD 배지에서 잘 생육하였고, R. oryzicola JSL-GRU-003를 포함한 3 균주는 5% NaCl을 함유한 YPD 배지에서 생육하였다.

Keywords

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