Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Screening of Extraction Solvent Condition of Fermented Rhus verniciflua Stem Bark by Antioxidant Activities

항산화활성 비교를 통한 발효 칠피의 추출용매 조건 탐색

  • Kim, Myeong-Ok (Department of Applied Plant Sciences, Kangwon National Univeersity) ;
  • Kim, Ju-Sung (Department of Applied Plant Sciences, Kangwon National Univeersity) ;
  • Sa, Yeo-Jin (Department of Applied Plant Sciences, Kangwon National Univeersity) ;
  • Jeong, Hyun-Ju (Department of Applied Plant Sciences, Kangwon National Univeersity) ;
  • Chun, Wan-Joo (Department of Pharmacology, Kangwon National Univeersity) ;
  • Kwon, Yong-Soo (Neurotoxicology Program, College of Phamacy, Kangwon National Univeersity) ;
  • Kim, Tae-Young (Fermentation & Food Processing Division, Department of Korean Food Research for Globalization, NAAS, RDA) ;
  • Choi, Han-Seok (Fermentation & Food Processing Division, Department of Korean Food Research for Globalization, NAAS, RDA) ;
  • Yu, Chang-Yeon (Department of Applied Plant Sciences, Kangwon National Univeersity) ;
  • Kim, Myong-Jo (Department of Applied Plant Sciences, Kangwon National Univeersity)
  • 김명옥 (강원대학교 식물자원응용공학) ;
  • 김주성 (강원대학교 식물자원응용공학) ;
  • 사여진 (강원대학교 식물자원응용공학) ;
  • 정현주 (강원대학교 식물자원응용공학) ;
  • 전완주 (강원대학교 의과대학) ;
  • 권용수 (강원대학교 약학대학) ;
  • 김태영 (농촌진흥청 국립농업과학원 농식품자원부 발효이용과) ;
  • 최한석 (농촌진흥청 국립농업과학원 농식품자원부 발효이용과) ;
  • 유창연 (강원대학교 식물자원응용공학) ;
  • 김명조 (강원대학교 식물자원응용공학)
  • Received : 2010.03.31
  • Accepted : 2010.07.28
  • Published : 2010.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we investigated antioxidant capacity and determined the phenolic and flavonoid contents using each of various solvent conditions from fermented Rhus verniciflua stem bark (F-RVS). Each extracts displayed markedly similar content of extraction yield. However, M80 extract showed a significantly higher antioxidant activity in comparison to other extract investigated. M80 exhibited strong DPPH radical scavenging activity with $RC_{50}$ value of $10.5{\pm}1.4{\mu}g/m{\ell}$, reducing power value 1.04 Abs (concentration of 1 mg/$m{\ell}$), and anti-lipid peroxidation activity value of 94.6% (concentration of 10 mg/$m{\ell}$). M80 extract showed the high content of total phenolic (319.7 mg GAE/$m{\ell}$ extract) and total flavonoid (111.6 mg QE/$m{\ell}$ extract). Phenolic and flavonoid compounds showed significant relationship in DPPH radical scavenging ($R^2$=0.911 and 0.912, each extract) and reducing power ($R^2$=0.903 and 0.837, each extract) from fermented R. verniciflua stem bark. However, antilipid peroxidation activity ($R^2$=0.589 and 0.441, each extract) was not significant like DPPH radical scavenging and reducing power. Therefor the result indicated that the potential antioxidant activities and functional values were ovserved significantly at M80 solvent condition from the fermented R. verniciflua stem bark.

Keywords

References

  1. Ahn EM, Park SJ, Choi WC, Choi SH and Baek NI. (2007). Antioxidant activity of isolated compounds from the heartwoods of Rhus verniciflua. Journal of Korean Society Applied Biological Chemistry. 50:358-361.
  2. An BJ, Lee JY, Park TS, Pyeon JR, Bae HJ, Song MA, Baek EJ, Park JM, Son JH, Lee CE and Choi KI. (2006). Antioxidant activity and whitening effect of extraction conditions in Curcuma longa L. Korean Journal of Medicinal Crop Science. 14:168-172.
  3. Bao W, O'Malley DM, Whetten R and Sederoff RR. (1993). A laccase associated with lignification in loblolly pine xylem. Science. 260:672-674. https://doi.org/10.1126/science.260.5108.672
  4. Blois MS. (1958). Antioxidant determination by the use of a stable free radical. Nature. 181:1199-1200. https://doi.org/10.1038/1811199a0
  5. Boo HO, Lee HH, Lee JW, Hwang SJ and Park SU. (2009). Different of total phenolics and flavonoids, radical scavenging activities and nitrite scavenging effects of Momordica charantia L. according to cultivars. Korean Journal of Medicinal Crop Science. 17:15-20.
  6. Choi HS, Kim MK, Park HS, Yun SE, Mun SP, Kim JS, Sapkota K, Kim S, Kim TY and Kim SK. (2007). Biological detoxification of lacquer tree (Rhus verniciflua stokes) stem bark by mushroom species. Food Science and Biotechnology. 16:935-942.
  7. Erkan N, Ayranci G and Ayranci E. (2008). Antioxidant activities of rosemary (Rosmarinus officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chemistry. 110:76-82. https://doi.org/10.1016/j.foodchem.2008.01.058
  8. Hong BK, Eorm SH, Lee CO, Lee JW, Jeong JH, Kim JK, Cho DH, Yu CY, Kwon YS and Kim MJ. (2007). Biological activities and bioactive compounds in the extract of Ace tegmentosum Maxim. stem. Korean Journal of Medicinal Crop Science. 15:296-303.
  9. Inatani R, Nakatani N and Fuwa H. (1983). Antioxidative effect of the constituents of rosemary (Rosmarinus offcinalis L.) and their derivatives. Agricultural and Biological Chemistry. 47:521- 528. https://doi.org/10.1271/bbb1961.47.521
  10. Jeong HS, Han JG, Ha JH, Kim Y, Oh SH, Kim SS, Jeong MH, Choi GP, Park UY and Lee HY. (2009). Antioxidant activities and skin-whitening effects of nano-encapsuled water extract from Rubus coreanus Miquel. Korean Journal of Medicinal Crop Science. 17:83-89.
  11. Jung CH, Jun CY, Lee SD, Park CH, Cho K and Ko SG. (2006). Rhus verniciflua stokes extract: radical scavenging activities and protective effects on $H_2O_2$-induced cytotoxicity in macrophage RAW 264.7 cell lines. Biological and Pharmaceutical Bulletin. 29:1603-1607. https://doi.org/10.1248/bpb.29.1603
  12. Kim IW, Shin DH and Choi U. (1999). Isolation of antioxidative components from the bark of Rhus verniciflua stokes screened from some Chinese medicinal plants. Korean Journal Food Science Technology. 31:855-863.
  13. Kim JS, Kwon YS, Chun WJ, Kim TY, Sun J, Yu CY and Kim MJ. (2010). Rhus verniciflua stokes flavonoid extracts have anti-oxidant, anti-microbial and $\alpha$-glucosidase inhibitory effect. Food Chemistry. 120:539-543. https://doi.org/10.1016/j.foodchem.2009.10.051
  14. Kim JY, Maeng YS and Lee KY. (1995). Antioxidative effects of soybean extracts by using various solvents. Korean Journal Food Science Technology. 27:635-639.
  15. Kim MJ, Choi YH and Kwak SS. (1998). Comparison of urushiol composition and biological activity between fresh sap and fire distilled sap of lacquer tree. Korean Journal of Plant Resources. 11:40-46.
  16. Kim SM, Cho YS and Sung SK. (2001). The antioxidant ability and nitrite scavenging ability of plant extracts. Korean Journal of Food Science Technology. 33:626-632.
  17. Koh YJ, Cha DS, Choi HD, Park YK and Choi IW. (2008). Hot water extraction optimization of dandelion leaves to increase antioxidant activity. Korean Journal of Food Science Technology. 40:283-289.
  18. Lee EJ, Kwon YI, Shetty K and Jang HD. (2004). Antioxidant activity of Rhodiola rosea extract on human low-density lipoprotein oxidation and DNA strand scission. Food Science and Biotechnology. 13:814-820.
  19. Lee SH, Kang KI and Lee SY. (2008). Saponin composition and physico-chemical properties of Korean red ginseng extract as affected by extracting conditions. Journal of Korean Society Food Science Nutrition. 37:256-260. https://doi.org/10.3746/jkfn.2008.37.2.256
  20. Lim KT, Chun H and Kitts DD. (2001). Antioxidant activity of a Rhus verniciflua stokes ethanol extract. Food and Chemical Toxicology. 39:229-237. https://doi.org/10.1016/S0278-6915(00)00135-6
  21. Moreno MIN, Isla MI, Sampietro AR and Vattuone MA. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  22. Oh HI, Lim TS, Lee GD and Kim HK. (2006). Establishment of microwave-assisted extraction conditions for antioxidative extracts from Pleurotus eryngii. Journal of Food Science and Nutrition. 25:1-6.
  23. Oyaizu M. (1986). Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition. 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  24. Park HJ, Kwon SH, Kim GT, Lee KT, Choi JH, Choi JW and Park KY. (2000). Physicochemical and biological characteristics of flavonoids isolated from the heartwoods of Rhus verniciflua. Korean Journal of Pharmacology. 31:345-350.
  25. Prasad KN, Xie H, Hao J, Yang B, Qiu S, Wei X, Chen F and Jiang Y. (2010). Antioxidant and anticancer activities of 8- hydroxypsoralen isolated from wampee [Clausena lansium (Lour.) Skeels] peel. Food Chemistry. 118:62-66. https://doi.org/10.1016/j.foodchem.2009.04.073
  26. Richard SK and Karyn LJ. (2001). Enrichment and function of urushiol-specific T lymphocytes in lesions of allergic contact dermatitis to urushiol. Journal of Immunology. 145:3706-3713.
  27. Taga MS, Miller EE and Pratt DE. (1984). Chia seeds as a source of natural lipid antioxidants. Journal of American Oil Chemical Society. 61:928-993. https://doi.org/10.1007/BF02542169
  28. Wettasinghe M and Shahdi F. (1999). Antioxidant and free radical-scavenging properties of ethanolic extracts of defatted borage (Borago officinalis L.) seeds. Food Chemistry. 67:399-414. https://doi.org/10.1016/S0308-8146(99)00137-5
  29. Yoon SH, Lim JH, Kim YS, Kim CH, Jo JH and Hwang YS. (1998). Pharmacological effects of proteoglycans extracted from fruiting bodies of Fomitella fraxinea. The Korean Journal of Mycology. 26:511-518.