Abstract

For the purpose of investigating the in vitro antidiabetic activity of a medicinal herb mixture prepared through traditional antidiabetic prescription, the study analyzed the existence of insulin-similar components and examined ${\alpha}$-amylase and ${\alpha}$-glucosidase inhibition activity. As a result of arranging the medicinal herb mixture extracts over the 3T3-L1 fibroblast in the concentration of $10{\mu}g/mL$, which confirmed that it included much of insulin sensitizer components as 151.7% in the differentiation of 3T3-L1 fibroblast. The inhibition activity against ${\alpha}$-amylase of the medicinal herb mixture extracts as hypoglycemic agent were 38.4, 31.5 and 16.6% in the concentration of 10.0, 1.0 and 0.1 mg/mL, respectively. The inhibition activity against ${\alpha}$-glucosidase of the medicinal herb mixture extracts were 81.3, 35.8 and 26.7% in the concentration of 10.0, 1.0 and 0.1 mg/mL, respectively. The inhibition activity against ${\alpha}$-glucosidase in the ethyl acetate fractions of the water and 80% ethanol extracts were 66.9% and 55.1%, respectively, the highest levels in the various solvent extracts.

본 연구는 당뇨처방을 근거로 구성된 생약재 복합물의 항당뇨 효능을 조사하기 위하여 복합물의 인슐린성 물질을 탐색하고 ${\alpha}$-amylase 및 ${\alpha}$-glucosidase 저해활성을 평가하였다. 3T3-L1 세포에 복합 생약재 추출물을 $10{\mu}g/mL$의 농도로 처리하였을 때 지방세포로의 분화능은 151.7%로 인슐린의 작용을 향상시키는 것으로 확인되었다. 혈당저하제로서 생약재 복합 추출물의 ${\alpha}$-amylase에 대한 저해활성은 생약재 복합 추출물 10.0, 1.0 및 0.1 mg/mL의 농도에서 각각 38.4, 31.5 및 16.6%이었으며, ${\alpha}$-glucosidase에 대한 생약재 복합 추출물의 저해활성은 같은 농도에서 각각 81.3, 35.8 및 26.7%였다. 열수 추출물과 80% ethanol 추출물의 각종 용매 분획물에 대한 ${\alpha}$-glucosidase 저해활성은 다양한 용매 분획물 가운데 ethyl acetate층에서 66.9%와 55.1%로 가장 높은 활성을 나타내었다.

Keywords

• medicinal herb mixture;
• hypoglycemic effects;
• ${\alpha}$-amylaseinhibition activity;
• ${\alpha}$-glucosidase inhibition activity;
• insulin-similar components