Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Free Radical-scavenging Activities of Amaranth (Amaranthus spp. L.) Seed Extracts

아마란스 종자 추출물의 라디칼 저해활성

  • Chung, Kang-Hyun (Department of Food Science and Technology, Seoul national University of Science & Technology) ;
  • Jo, Hyeon-Ju (Department of Food Science and Technology, Seoul national University of Science & Technology) ;
  • Yoon, Jin-A (Department of Food & Nutrition, Baewha Women's University) ;
  • Song, Byeong Chun (Division of food Bioscience, Konkuk University) ;
  • An, Jeung Hee (Division of food Bioscience, Konkuk University)
  • 조현주 (서울과학기술대학교 식품공학과) ;
  • 정강현 (서울과학기술대학교 식품공학과) ;
  • 윤진아 (배화여자대학교 식품영양과) ;
  • 송병춘 (건국대학교 식품생명과학부) ;
  • 안정희 (건국대학교 식품생명과학부)
  • Received : 2014.03.06
  • Accepted : 2014.04.16
  • Published : 2014.05.31
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Abstract

This study investigated the antioxidant activities of Amaranth (Amaranthus spp. L.) seed extracts. Methanol and hot water extracts of the seeds were evaluated for their total polyphenol, flavonoid, and tannin content, antioxidant activities, and protective effects against oxidative stress on L-132 cells. The hot water extracts showed higher total phenol (4.23 mg GAE/g) and tannin (0.511 mg TAE/g) content, while the methanol extracts showed higher flavonoid content (1.53 mg CE/g). The methanol extracts performed better than the water extracts for DPPH radical-scavenging activity, while the water extracts performed better for ABTS radical-scavenging activity and SOD-like activity. With L-132 cells, the water extracts showed strong protective effects against oxidative stress in a dose dependent manner with an effect three-fold higher than that of the methanol extracts at a concentration of $500{\mu}g/mL$. A higher inhibition activity of nitric oxide in RAW 264.7 cells was observed with the water extracts than with the methanol extracts at a concentration of $250{\mu}g/mL$. In summary, the water extracts showed the highest total polyphenol and tannin content and a more powerful protective effect against oxidative stress and inhibition effect of NO. Our results suggest that amaranth seeds may be useful as natural antioxidant compounds.

본 연구는 아마란스 종자 물 추출물과 메탄올 추출물의 폴리페놀과 플라보노이드 함량 측정과 DPPH와 ABTS 라디칼 소거활성, SOD 유사활성을 측정하였으며 $H_2O_2$에 의한 산화적 스트레스에서의 세포 보호효과와 산화질소 생성억제활성을 분석하여 새로운 식물유래 라디칼 소거활성 물질을 개발하기 위하여 시행하였다. 물 추출물의 총 폴리페놀 함량은 4.23 mg GAE/100 g이고 탄닌 함량은 0.511 mg TAE/g으로 메탄올 추출물보다 높았다. DPPH 라디칼 소거활성은 $50{\mu}g/mL$의 농도에서 메탄올 추출물 추출물(5.8%)이 가장 좋았으며 물 추출물의 활성은 $250{\mu}g/mL$의 농도에도 활성을 보이지 않았다. ABTS 라디칼 소거활성에서 메탄올 추출물은 $250{\mu}g/mL$의 농도에서 활성이 11.1%로 가장 좋았으며 물 추출물에서는 $50{\mu}g/mL$에서 11.8%의 활성을 나타내었다. SOD 유사활성에서 메탄올 추출물은 $100{\mu}g/mL$ 농도에서 14.04%으로 가장 높은 활성이 나타났으며 물 추출물은 $50{\mu}g/mL$ 농도에서 18.34%의 활성이 나타났다. 아마란스 종자 물 추출물의 경우 산화적 스트레스($H_2O_2$)에 의한 세포 보호 효과를 갖는 것을 확인하였다. 세포내 NO 생성 억제활성을 조사한 결과에서는 $250{\mu}g/mL$의 농도에서 물 추출물이 51.9%의 가장 높은 억제활성을 보여주었다. 본 연구의 결과, 폴리페놀과 탄닌의 함량이 높은 물 추출물에서 라디컬 소거능은 낮았으나 산화적 손상에 대한 보호 효과와 NO생성억제능이 높아 강력한 항산화제로써의 활성을 보여주었다. 이러한 결과로 보아 아마란스 종자의 새로운 항산화소재로서 개발가능성을 보여주었다.

Keywords

Acknowledgement

Supported by : 서울과학기술대학교

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